显微观察

Using transmission electron microscope, I had found that there were obvious differences in the content of chloroplast and amyloplast and in the structure of grana lamella between transformed and wild tobaccos.

以转基因植株为材料，对植株叶片进行亚显微结构的透射电镜观察，发现野生型烟草与转基因烟草中的叶绿体、淀粉粒含量以及叶绿体中的基粒片层结构明显不同。

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Objective To investigate the effect of transplantation of limbal autograft following microsurgery for pterygium.

目的 观察各种类型翼状胬肉显微手术切除后自体角膜干细胞移植的临床疗效。

ABSTRACT］ Objective: To investigate the effect of transplantation of limbal autograft following microsurgery for pterygium.

导读：观察各种类型翼状胬肉显微手术切除后角膜干细胞移植的疗效。

In this thesis, the dynamic processes of boron segregating to grain boundaries during cooling in Fe-40%Ni alloy are first studied by boron autoradiography technique and transmission electron microscopy.

本文首先应用硼径迹显微技术和透射电镜观察了Fe-40%Ni合金在连续冷却过程中硼向晶界非平衡偏聚的发展过程。

Embryo development and origin of twin-embryo of autotetraploid rice with twin-embryo seedlings were observed and verified.

利用激光扫描共聚焦显微技术对同源四倍体双胚苗水稻的受精过程、胚胎发育过程和双胚来源进行观察鉴定。

The bulbs of the Lycoris radiatat were pretreated with comminution, ultrasonic and basification methods. The effect of the pretreatment was studied, via observing microscopic character of the cell wall fragmentation of Lycoris radiatat and the yield of galanthamine with SC-CO2 extraction.

采用粉碎、超声波和碱化预处理方法处理石蒜鳞茎原料，通过对原料细胞壁的破碎状况的显微特征观察，以及SC-CO2提取加兰他敏提取率的变化考察了预处理的效果。

Methods (1)We observed the serial sheet sections of the optic apex region obtained using collodion microtomy in 4 adult cadaver heads;(2)High resolution CT scanning and three dimensional images of the optic apex region were observed and measured in 10 adult volunteers;(3)High resolution magnetic resonance images of the optic apex region were obse.

①采用火棉胶切片技术对4例(8侧)成人眶尖区标本进行连续薄层断层解剖学观察；②使用高分辨力CT及三维重建技术观测10例(20侧)志愿者眶尖区；③观测5例(10侧)志愿者眶尖区高分辨力MRI断层扫描图像；④对30例(60侧)成人干颅骨标本和15例(30侧)成人湿性头颅标本的眶尖区结构进行显微解剖；⑤在手术显微镜和内窥镜下对3例成人头颅湿性标本模拟眶内经颅入路。

According to the micrograph of the composite cross-section, the basic hypothesis related to the yarns configuration is presented. Based on the structural parameters of the three-dimensional woven structure, a unit cell structural geometrical analysis model is built up, and analyzes the linear-elasticity mechanical performance using averaging stiffness approach.

基于对复合材料断面显微照片的观察，提出了关于各向纱线形态的基本假设，结合织物的结构参数，建立了三维正交机织物细观几何结构分析模型，并在此基础上，运用刚度平均法分析了三维正交机织复合材料的线弹性力学性能。

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术，观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响，结果证实了促孕液治疗卵巢静止、持久黄体的机理：(1)微弱的雌激素样作用，能刺激卵泡发育，促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化；(2)提高子宫内膜碱性磷酸酶的活性；(3)改善微循环；(4)促进子宫运动。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。