显微观察

Chapter Three. The Studies on Gene Therapy for Retinal Degeneration of the Mice and It's Mechanism Objective: To evaluate the abilities of adeno-associated virtus carrying the basic fibroblast growth factor gene as a therapy for retinal photoreceptors in slow retinal degerneration mice on the basis of the experiment results as mentioned above. Methods: On the born day of rds mice, IPE cells were isolated with enzyme-assisted microdissection from pigs and were cultured.

第三章 视网膜色素变性小鼠的基因治疗及其机制目的：在体外实验证明AAV载体可以高效安全转染IPE细胞并且可以表达具有生物活性的bFGF的基础上，应用AAV载体携带bFGF基因以先体外，后体内法和体内法两种方式进行视网膜色素变性鼠(retinal degemeration slow，rds)的基因治疗，在分子生物学、形态学和功能学上进行基因治疗的初步观察方法：在rds鼠出生的当天取眼球，采用酶辅助的显微分离方法分离培养IPE细胞。

A layer of strip steel was found abrasion-welded to the surface of the spalled fragment and a 0.06mm thin layer of untempered martensitic structure was observed on the surface of the roll just beneath the welded strip.

在剥落块的表面上粘结着带钢，在带钢下的轧辊表面上观察到一层厚度约0.06mm未经回火的马氏体组织，此层下面还有一个具有低硬度的回火马氏体区域，晶间显微裂纹就从此两区域之间发生，然后垂直地向里向外扩展。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

To address this issue, we microinjected Morpholino Oligonucleotides, which acts as a specific translational inhibitor, into 1-cell stage zebrafish embyos to knockdown the expression of FoxD5. Results showed that the newly forming somites of the FoxD5-MO-injected embryos were malformed both in the stage when the somitogenesis was completed (24-hpf) and in the stage when the somitogenesis was in progress (14-hpf).

在本篇研究中，为了了解FoxD5在体节生成时的功能，利用专一抑制基因转译之Morpholino Oligonucletides对1-cell时期之斑马鱼胚胎进行显微注射以抑制FoxD5的转译，并在体节发育已完成之24 hpf时、及体节仍在形成之14 hpf (约12-somite stage)时分别观察FoxD5-MO注射个体(FoxD5 morphants)，发现新生成之体节处有产生体节发育畸形的现象。

We reported the investigation results of the magnetic domain s in a Co_(50)Ni_(20)Ga_(30) shape memory alloy with applying a magnetic field by objective lens with the out-of-focus methods of Lorentz microscopy.

本文首次通过增加物镜电流对样品施加外加磁场，并利用洛伦茨显微术实时观察了在外加磁场作用下Co50Ni20Ga30合金的磁畴结构的变化。

Microstructure of cladding layer was observed and analyzed with the SEM, and the micro-hardness was measured. The results were contrasted with the Ni60 coating remelted by oxygen-acetylene spraying.

用扫描电镜对熔覆层组织特征进行观察分析，用显微硬度测试仪进行硬度测试，与Ni60氧－乙炔火焰喷焊涂层进行了对比。

In the ferrous martensite transformations, the lath martensite of substructure with high-density dislocations is made up of the transformation basic-units with same orientations whereas the plate-shaped martensite with twin substructure is made up of the basic-units with different orientations.

本文根据铁基合金马氏体和贝氏体显微组织观察以及晶体学位向关系测定，提出板条马氏体、孪晶马氏体和贝氏体铁素体均由相变基元构成，相变基元具有切变形核长大的晶体学特征。

The morphology of electroless platings of Ni P UFD was studied by optical metallographic microscopy and SEM, the age temperature's effect on the microstructure of this platings was studied using XRD, TEM and DTA, and the age temperature's effect on microscopic hardness was explained.

利用光学金相显微镜和 SEM观察了 Ni-P-超细金刚石化学复合镀层形貌，用 XRD、TEM及 DTA研究时效温度对镀层组织结构的影响，并解释了时效温度对镀层显微硬度的影

This study presented the effect of multi-modification on microstructure and properties of high manganese steel by means of microstructure observation, X-ray diffraction line profile analysis and properties testing.

通过显微组织观察，X射线线形分析和性能测试，研究了多元变质处理对高锰钢微观结构和性能的影响。

The Observation of Microstructure of Tool steel.

实验二 工具钢的显微组织观察。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。