显微注射

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

3D control of micropositioning, the environment for human-computer interaction based on the micro stereo image displaying, and microinjection are three focuses of this dissertation.The imaging models of CMO micro stereoscope and G micro stereoscope used in microvision subsystem are deduced. According to the imaging models, 3D visual servoing model based on image for micropositioning is set up. The technology of tracking and matching on the feature of image, which are relevant in visual servoing, is studied. Two arithmetic for avoiding obstacles, rolling/surrounding and curved tracking based on the depth first search, are further discussed.

在此基础上，对三维微定位控制技术、基于显微立体图像显示的人机交互操纵环境和微量注射等三个内容进行了重点研究：为了解决3D微定位问题，推导了显微视觉分系统采用的CMO型和G型体视显微镜光学成像模型，在此基础上建立了基于图像的3D显微视觉伺服定位控制模型，对视觉伺服中涉及的实时跟踪与立体匹配技术进行了研究，并进一步探讨了卷包裹法和曲线跟踪两种避障算法。

The rate of Flow cytometry to analyse retinoic acid haploid is 19.3%.The sperm-like cells achieved could activate bovine oocytes by sperm injection, and the development rate of blastosphere was 20.36%. Conclusions SSCs of newly -born mice can be induced and differentiated into sperm-like cells and are also capable of activating oocytes.

本实验通过体外诱导新生小鼠SSCs分化为精子样细胞，探讨不同诱导方法的诱导效率；采用显微注射法将精子样细胞注射到卵母细胞和去核卵母细胞，探讨其体外重构胚的发育能力，为SSCs体外分化研究打下基础。

The effects of BN content on the thermal conductivity, hardness and microstructure of AlN-BN composite ceramics prepared by ceramic injection molding and pressureless sintering, as well as the rheological characteristics of the mixtures were discussed.

采用粉末注射成形和无压烧结相结合的工艺制备AlN-BN复相陶瓷，讨论了AlN-BN混合料的流变性能以及BN含量对复相陶瓷热导率、硬度以及显微组织的影响。

The vector was linearized and injected into 817 fertilized eggs of mice in which coagulation factor IX gene has been knocked out. The manipulated embryos were transferred into the oviducts of 45 pseudopregnant females, from which 63 offsprings were obtained.

将其线性化后，用显微注射法注射入817只凝血因子Ⅸ基因剔除小鼠受精卵雄原核，再将它们分别回输45只假孕受体母鼠的输卵管中，共产仔69只，存活63只。

Methods Healthy Sprague-Dawley rats received basal ovalbumin-sensitized combined with intra-esophageal mucosal acid exposure. The neuronal expressions of c-fos proto-oncogene were detected with immunohistochemical counter-staining and computerized color image analyzer.

采用腹腔注射鸡卵清蛋白基础致敏联合食管酸灌注的方法处理SD大鼠，并采用免疫组织化学方法和显微图像分析技术评价内脏高敏感性-食管化学刺激大鼠模型的可靠性。

Before cell injected, the error matrix is calculated from servoing control equation to calibrate injection system , by which the coordinate transform error between vision system and micromanipulator system is minished.

细胞注射前，利用伺服控制方程计算的误差矩阵对系统进行标定，减小显微视觉系统和机械手系统间的坐标变换误差。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。