显微注射

In the former group,28 rats were injected into the cisterna magna with 0.05ml volume of 2% kaolin solution under microscope.

实验组28只大鼠应用显微外科技术在显微镜下向枕大池中注射2%高岭土混悬液0.05mL，对照组12只大鼠应用显微外科技术在显微镜下向枕大池中注射生理盐水0.05mL。

This study retrospectively analysed the clinical data of 1134 cases after IVF-ET and 317 cases after cryopreservation and thrawing embryo transfer.

回顾性分析7年间施行IVF-ET(包括卵胞浆内单精子显微注射)1134个周期及冻融移植317个周期的资料，分析不孕原因及胚胎移植距宫底的深度与异位妊娠的关系。

It introduces some equipments for experiments, and some experiments on worms, such as the microinjections experiment under microscope, the stainings experiment of worms' cell, the mutants of worms, drugs experiment on worm and so on.

它介绍了一些实验设备的基本情况，以及一些有关蠕虫的实验，如在显微镜下进行的显微注射实验、对蠕虫细胞的染色实验、基因突变体、药物实验等等。

Methods:The HBVtransgenic mice were prepared by microinjection of complate 2.0 copy HBV genome into pronuclei fertilized eggs.

应用受精卵原核显微注射的方法，产生HBV转基因小鼠。

Offspring of the fertilized eggs whose pronuclei were injected of the plasmid were screened for transgene expression.

接著此载体以显微注射递送到小鼠受精卵中，其子代以聚合脢链锁反应筛选转殖基因是否嵌入其基因体。

Construction of heavy chain transgenic mice and genome type analysis Linearized transgene plasmids were microinjected into pronuclei of zygotes from CBA×C57BL/6 mice, and transplamted into oviduct of pseudo-pregnant mice.

二、抗体重链转基因小鼠建立及基因型分析将线性化的质粒显微注射入CBA×C57BL/6小鼠的受精卵细胞的雄前核内，然后导入假孕雌鼠的输卵管。

We spliced the pronuclei vector and microinject the remain DNA sequence to pronuclei of the mouse zygote.We got 29 mice of G0 generation ,and 8 of the 29 mice are positive result which checked by method of PCR.

构建的乳腺特异性表达载体去除原核部分(αS1-casein基因调控序列+人胰岛素原基因)，显微注射小鼠受精卵雄原核，获得29只G_0代小鼠，PCR检测出其中8只为转基因刚性小鼠。

6 Kb of pGFAP-Cre-hGH DNA fragment was introduced into 191 fertilized eggs by microinjection. 176 injected eggs were implanted into the oviducts of 8 female mice respectively, from which 25 offspring were obtained.

以显微注射的方法将7.6 kb的转基因片段pGFAP-Cre-hGH引入191枚小鼠基因组受精卵，其中176枚分别移植至8只假孕母鼠的输卵管中使其发育，共获得子代小鼠25只。

6 Kb of pGFAP-Cre-hGH DNA fragment was introduced into 191 fertilized eggs by microinjection. 176 injected eggs were implanted into the oviducts of eight female mice respectively, from which 25 offspring were obtained.

以显微注射的方法将7.6 kb的转基因片段pGFAP-Cre-hGH引入191枚小鼠基因组受精卵，其中176枚分别移植至8只假孕母鼠的输卵管中使其发育，共获得子代小鼠25只。

The pregnant rates were 18.18% and 25% respectively after transferred to donors and receptors with natural estrus.

显微注射后胚胎移植给供体或自然发情的受体，移植受胎率分别为18.18%和25.00%。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。