显微注射

After microinjection of the pEGFP-C1 vector in to fertilized zebra fish eggs, the expression rate was 80%.

在显微注射绿萤光蛋白进入斑马鱼授精卵后具有80%表现率。

Microinjection and particle gun were used to transfer pCMV-β into fertilized zebra fish eggs. After staining analysis, the data showed80% and 6% expression rates, respectively.

此研究利用显微注射与粒子枪基因转殖法转殖pCMV-β质体进入斑马鱼授精卵，经染色分析得到80%与6%之表现率。

Objective: To study the clinical effect of intracytoplasmic sperm injection in treatment of oligozoospermia, azoospermia and teratozoospermia.

目的：探讨采用卵胞浆内单精子显微注射治疗少、弱、畸精子不育症的临床效果。

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鲤科鱼类Beta-肌动蛋白基因序列，采用长片段PCR技术，克隆了青鱼β-actin基因DNA片段，并构建了青鱼β-actin基因5'启动调控区绿色荧光蛋白表达载体，通过显微注射泥鳅胚胎和COS-7细胞转染实验，证明了所克隆的青鱼β-actin基因5'启动调控区具有启动调控功能，并用之来构建&全鱼&基因的启动子部分。

We generated EG4-GFP cell lines expressing enhancing green fluorescence protein which still maintained typical characteristics of pluripotent stem cells. We microinjected green cells into perivitelline of 8-cell stage embryos as well as aggregated them to 8-cell stage embryos with zona pellucida removed, to investigate developmental fate of EG4-GFP cells during formation of chimeric blastocysts.

我们建立了稳定表达绿色荧光蛋白的EG细胞系（EG4-GFP），发现该细胞仍能保持野生型的一般生长特性；通过显微注射和体外聚集得到细胞与8-细胞受体胚胎共同发育形成的嵌合胚，发现转基因细胞参与了胚胎早期发育过程中的生长与分化，大部分细胞集中于内细胞团，也有细胞分化为滋胚层细胞。

Development ability of sheep embryos after pronuclear injected withDNA was studied.

研究了显微注射对绵羊体外受精胚胎发育的影响。

Intracytoplasmic sperm injection is a very important assistant reproductive technology for treatment of man infertility and fundamental research , Success in ICSI in human and other animals has recently been achieved., The present pilot studies were conducted to establish a preliminary procedure for ICSI in mice, explore the experimental conditions of ICSI in mice, investigate factors influencing the test results, and accumulate technologic basis for the next studies on pronuclear exchange in mice .

本部分研究论文初步建立了小鼠ICSI技术的基本操作程序，摸索了小鼠ICSI研究的操作条件，总结了一些影响显微注射效果的重要因素，并为下一部分小鼠原核置换的研究奠定了雄厚的技术基础。

Conditional rBDNF transgenic vector was successfully constructed and strong overexpression of rBDNF and EGFP were identified in the cotransfected cell line, and establishment of this targeting vector is critical for pronuclear microinjection.

rBDNF条件性基因载体构建成功，在体外细胞系中，检测到rBDNF及EGFP强表达，为转基因显微注射打下基础。

We microinjected one of the double positive ES cell clone into the mouse blastocysts (3.5days), cultured for 4-5 hours, then implanted the blastocysts in the uteri of the pseudopregnant mice.

选择其中的一个多能胚胎干细胞显微注射到孕3.5天的小鼠囊胚内，培养4-5小时后，将囊胚移植入假孕小鼠的子宫中。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。