显微注射

The purity of foreign DNA plays an important role for the success of microinjection.

本文介绍用氯化钠密度梯度离心的方法制备用于显微注射的外源DNA片段。

Gene microinjection and integration are the most important ways to make transgenic animals in C. elegans . They are widely used in the studies of expression, function and interaction of genes.

其中基因显微注射和整合是该领域的核心技术，广泛应用于研究线虫的基因表达、功能和基因间的相互作用等。

Whole mount in situ hybridization and transgene via microinjection are two important methods in zebrafish study.

整胚原位杂交和显微注射转基因技术是斑马鱼基因功能研究中两种重要的研究方法。

In this study,we introduced the use of sodium chloride step gradients in fractionating foreign DNA fragment for microinjection.

与传统的琼脂糖凝胶回收的方法相比较，用此方法制备的外源DNA片段对小鼠受精卵进行显微注射后，受卵体母鼠的胚胎存活率，以及子代小鼠的外源基因整合率均有明显的提高。

Lower concentration of the microinjected DNA, choosing CZB and zygotes with good quality can enhance efficiency of microinjection.

选用较低的基因浓度，良好的操作介质CZB，使用质量好的受精卵，会提高显微注射的效率。

Results In the transgenic mice after microinjection, there were 4 Southern-blot positive mice, and the expression of bcl-xL mRNA and protein was detected.

结果 在显微注射所产生的转基因小鼠中，有4只为Southern blot检测阳性，并且在这4只小鼠及其子代中均检测到人bcl-xL的mRNA和过表达的目的蛋白。

Then the linear vector was microinjected into the pronucleus of the KM×B6D2F1 zygotes to produce the transgenic mice. Thirty-four adult mice were produced from 286 of injected embryos.

用显微注射方法，将线性化的载体DNA注入KM ×B6D2F1小鼠受精卵的的原核内部，然后将胚胎移植到输卵管内，13只受体怀孕产仔。

The appearance of Round spermatid injection provides a new way to treat the sterile human male .

圆形精细胞卵胞质内显微注射(Round spermatid injection，ROSI)辅助生殖技术的出现为治疗人类男性不育提供了新的途径。

Objective:To compare the efficacy of 2 revised long protocols by triptorelin in in vitro fertilization and embryo transfer and intracytoplasmic spermatocyte injection.

目的：比较两种修正的达必佳长方案在体外受精-胚胎移植和卵母细胞单精子显微注射中的作用。

There are significant differences between the two methods. Conclusion Clysising fimbriae tubae is a better embryo collecting method for rat microinjection.

冲洗输卵管伞端采集胚胎的方法为大鼠显微注射前胚胎采集比较好的方法。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。