显微注射

Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接，并接入表达载体pcDNA 3.1中，构成含hMCP-DAF的真核表达质粒(pcDNA-MD3)；以脂质体转染猪内皮细胞，人血清处理阳性克隆细胞，鉴定抑制人补体溶破的功能；应用显微注射将线性基因导入猪受精卵；PCR检测导入基因整合率。

The highest translation efficiency was observed when BBO were cultured in Barth's buffer at 25℃.

显微注射mRNA的BBO用Barths液经20℃培养可获得最高翻译效率。

Forced expression of fez induces ectopic expression of dlx2 and dlx6, two genes involved in brain development.

通过显微注射含有Fez基因的DNA或mRNA使该基因在野生型斑马鱼胚胎中超表达，可以诱导两个与脊椎动物前脑发育相关的基因dlx2和dlx6发生提前和异位的表达。

With the development of biotechnology, the technology of microinjection has been widely applied in the area of cell manipulation.

随着生物技术的发展，显微注射技术在细胞操作方面得到了广泛的应用。

However, the mice were dead immediately after delivery and all the mice had defect in cerebral development similar to microcephaly.

於显微注射与胚胎转移后，成功产出的MLL基因转殖鼠都有头部畸型产生，初步判定为小头症伴随大脑发育障碍，同时小鼠於出生后立即死亡。

In this paper, the applied research of cell microinjection is based on the micro-fluidic digital technology above.

本论文是上述微流体数字化技术在细胞显微注射技术上的实验研究。

Here, the detail processes and key points of microinjection and gene integration in C.

显微注射是一项精细的实验技术，影响实验成功与否的因素较多，实际操作需要有一定的经验。

Objective To study the integration, transcription and expression of human bcl-xL gene in the transgenic mice generated by microinjection.

目的 研究人bcl-xL基因在显微注射所产生的子代小鼠中的整合、转录和表达情况。

Methods: Microinjection technique was used to structure the human SERT heterologious system.

显微注射方法将 hSERT 基因 cRNA 注射到非洲爪蟾卵母细胞内，建立异体表达系统。

To address this issue, we microinjected Morpholino Oligonucleotides, which acts as a specific translational inhibitor, into 1-cell stage zebrafish embyos to knockdown the expression of FoxD5. Results showed that the newly forming somites of the FoxD5-MO-injected embryos were malformed both in the stage when the somitogenesis was completed (24-hpf) and in the stage when the somitogenesis was in progress (14-hpf).

在本篇研究中，为了了解FoxD5在体节生成时的功能，利用专一抑制基因转译之Morpholino Oligonucletides对1-cell时期之斑马鱼胚胎进行显微注射以抑制FoxD5的转译，并在体节发育已完成之24 hpf时、及体节仍在形成之14 hpf (约12-somite stage)时分别观察FoxD5-MO注射个体(FoxD5 morphants)，发现新生成之体节处有产生体节发育畸形的现象。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。