显微注射

DNA microinjection is the most popular and reliable method of producing transgenic animals.

DNA显微注射是生产转基因动物最可靠和最常使用的一种方法，外源DNA的纯度对显微注射的成功起着至关重要的作用。

Microinjection experimentation proved that both expression rate of marker gene and the survival rate of transgenic embryos are high.

显微注射实验证实：在青鳉受精卵的单细胞期进行显微注射，标记基因的表达率和胚胎的存活率均较高。

Lamin A gene cDNA lacking of 150 basyls at the end of the eleventh extron was cloned into pcDNA3.1 carrier, and the recombinant plasmid pcDNA3.1-mutant LMNA cDNA was injected into the male pronucleus of mouse zygotes, then the zytotes was replanted into the oviducts of pseudopregnant mice.

实验方法：将缺失了第11外显子末端150个碱基的LMNA基因cDNA克隆到pcDNA3.1载体，应用原核显微注射方法将线性化的pcDNA3.1-mutant LMNA注射到小鼠受精卵的雄原核，再将注射后的受精卵移植至假孕鼠的输卵管。

The toxicity of cryoprotectans increased as the concentration of its increased.3, the survival rate of embryos microinjected with 6M PM was 25.07±1.57% after being coolled in -20℃ for 10 minutes by programmed cooling method, whenas ,the survival rate of controls dealt with five steps balance method was 20.88±2.84%, which indicated that the chilling sensitivity of embryos with microinjection decreased.

而任何一种抗冻剂均随着浓度的增加其毒性随之增加。3、另外，在冷敏感实验中，显微注射6M的PM的胚胎胚胎，应用程序化法处理，以2℃/min的速率降至-20℃，平衡10min后解冻处理，发现注射PM的胚胎低温处理后成活率为25.07±1.57%，而6M的PM五步平衡法同步处理的对照组胚胎成活率为20.88±2.84%，说明显微注射的胚胎冷敏感性一定降低。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

This experiment is for the purpose of unifying the porine ovocyte in vitro mature raise and the ROSI correlation operation, establishes in the ovicell nature the round sperm cell microinjection fertilization technology procedure, the exploration porine round sperm cell micro fertilization possibility, the research affects this kind of special cell microinjection fertilization effect the factor, for further studies the porine ROSI technology to provide the basic data.

本试验旨在结合猪卵母细胞体外成熟培养及ROSI相关操作，建立卵胞质内圆形精细胞显微注射受精技术程序，探索猪圆形精细胞显微受精的可能性，研究影响这种特殊细胞显微注射受精效果的因素，为进一步研究猪ROSI技术提供基础资料。

To establish a mouse microinsemination model, Intracytoplasmic sperm injection and subzonal sperm injection were studied use mouse gamets.

我们用小鼠卵细胞胞质内精子注射和透明带下精子注射的方法，对显微授精过程进行了初步探讨，对配子的采集和准备、精子的优化及显微注射的操作等作了阐述。

It is tentative investigated the transferring of mitochondria by microinjection. It is found that the key of the success of transferring of mitochondria by microinjection is not the microinjected from pollen grain trema, but the cultivated of the microinjected pollen grain.

对显微注射转移植物线粒体的技术进行了探索性研究，结果表明，该技术的难点不在通过花粉粒萌发孔进行mtDNA的显微注射，而在注射后花粉粒的培养。

When the other conditions were stable, the effect of different concentration of the microinjected DNA, and different timing of micromanipulation in CZB medium, different quality of zygotes on microinjection was investigated in vitro. Results: The higher the concentration of the microinjected DNA, the worse the development of zygotes.

获取小鼠受精卵，在其他条件固定的情况下，分别比较不同的注射基因浓度，在CZB操作液中显微操作时间以及注射前受精卵的质量对显微注射后的胚胎体外培养结果的影响。

During the course of mice ICSI,some critical factors were realized on influencing the experimental results, including micromanipulation systems, diameters of injection pipettes, concentrations of PVP and positions of the first polar body located in oocyte atlCSL

另外，本研究论文还研究了一些对显微注射结果有影响的因素，包括操作液PVP液的浓度、显微注射时卵母细胞第一极体的位置以及显微操作系统等。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。