显微操作的

And thaw, and cell fusion; Understanding the principles and operation procedures for autoradiograph; Mastering the skills of writing experiment reports and graphic drawing under microscope.

本课程是农业与生物学院本科学生的一门实验课程，本课程主要内容包括：掌握和了解普通光学显微镜、几种特殊显微镜和电镜的一般原理和使用方法；掌握显微镜临时标本的制备，会进行显微测量、细胞计数和染色体观察；初步观察细胞生理现象、细胞化学成分显示、染色体标本制备；掌握细胞培养、细胞冻存与复苏、细胞融合等技术的原理和方法；了解细胞放射自显影技术的原理和操作步骤；掌握实验报告的书写和镜下绘图的技能。

Here, the detail processes and key points of microinjection and gene integration in C.

显微注射是一项精细的实验技术，影响实验成功与否的因素较多，实际操作需要有一定的经验。

The calculation of carbon content of unconsumed pulverized coal in the dust under different PCI rate was carried out based on microscopic and chemical analysis results.

根据岩相显微分析和化学分析的结果，计算了宝钢高炉不同煤比操作条件下，炉尘中未消耗煤粉中的碳含量。

Rationale: Fibered confocal fluorescence microscopy is a new technique that produces microscopic imaging of a liing tissue through a 1-mm fiberoptic probe that can be introduced into the working channel of the bronchoscope.

原理：纤维共聚焦荧光显微镜这项新技术，是通过将1mm的纤维光学探头插入支气管镜的操作管道，进行活体组织的显微成像

Objective Sensory recordings were made in sciatic nerve of rabbits with self-made intrafascicular electrodes to discover more methods on harvesting information of peripheral nerve. Methods Recording electrode fabricated from external insulated wire 60μmin diameter（95%Pt-5%Ir alloy）,was implanted directly by microsurgery technique into the fascicle of the sciatic nerve of rabbit.

目的 应用自制神经束内电极采集家兔坐骨神经感觉信号以探索获取周围神经信息的方法方法用显微外科操作技术将直径60μm的95%铂、5%铱合金表面绝缘的金属丝直接插入家兔坐骨神经神经束中作记录电极。

The microsurgical techniques require gentle and careful operation in the establishment of ROLT. The rate of hemorrhage was very slow by reserving "phrenic ring" in the anastomoses of suprahepatic inferior vena cava.

应用改良的&二袖套法&建立稳定的大鼠肝移植模型需要轻柔细致的显微手术操作，肝上下腔静脉吻合时保留隔肌环可降低吻合口出血的发生率。

The use of free vascularized autografts has limited indications, requires expert microvascular technique, and is not without donor site morbidity.

带血管自体骨游离移植的适应证有限，手术操作也需要良好的显微外科技术，并且供区可能出现后遗症。

The use of free ascularized autografts has limited indications, requires expert microascular technique, and is not without donor site morbidity.

带血管自体骨游离移植的适应证有限，手术操作也需要良好的显微外科技术，并且供区可能出现后遗症。

With the advent of nano era, atomic force microscopes have been widely adopted as the major tool for analyzing or discovering new phenomena in research labs. However, it is clear that the environmental conditions like vibrations, changes in temperatures, noises etc., as well as post signal processing, can always affect the images of an AFM.

中文摘要随著奈米科技的进步，利用原子力显微仪做为主要的研究工具，来发现或验证新的物理及化学现象，成为不可避免的趋势，然而，对於 AFM 本身的制作开发，其周边环境因素的控制（如振动、温度、干扰杂讯等）、操作介面及讯号撷取后之处理，甚至探针的制作研究等，都是影响 AFM显像品质的重要影响因子。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。