显微操作的

Lower concentration of the microinjected DNA, choosing CZB and zygotes with good quality can enhance efficiency of microinjection.

选用较低的基因浓度，良好的操作介质CZB，使用质量好的受精卵，会提高显微注射的效率。

In this paper, the system construction of a micromanipulation robot based on hybrid control of micro-vision and micro-force was introduced, and the micro-vision system and micro-force system and their principle were described.

简单介绍了一种基于显微视觉和微力觉柔顺混合控制的微操作机器人的系统构成，并重点介绍了显微视觉系统和微力控制系统的组成及工作原理。

The process planning for the task of transgenosis manipulation is designed aiming at characteristics of the micro visual servo based micromanipulator.

针对建立的基于显微视觉伺服的微操作机器人特点，设计了细胞转基因操作任务的过程规划。

The first operational control element (28) has two control modes, i.e. a standard mode and a second mode, in order to provide an excellent degree of comfort when using the microtome.

为了实现显微切片机的良好操作舒适性，第一操作单元(28)具有两种控制模式，即标准模式和第二模式。

Dogs were selected as experimental subjects. Using micrurgy to destroy the valves in both sides of common iliac veins, external iliac veins, femoral veins and popliteal veins, establishing theanimal model of refluxing.

取杂交犬6条，全身肝素化，采用显微操作方法破坏每条犬的双侧髂总静脉、髂外静脉、股静脉和膕静脉内的生理瓣膜，构建下肢返流性疾病的动物模型。

Based on the basal manipulation and spatial arrangement of the microsurgery, dovetailed the vein during microsurgery manipulation as practical application object, the action of the doctor is recorded and quantified analyzed.

基于显微外科手术的基本操作和空间布局，以血管吻合显微手术操作为实际应用对象，对医生的动作进行了记录和量化分析，以此为标准设计了平面关节型的从手操作器。

Monozygotic mouse twins were produced by micromanipulation of 2cell stage embryos and factors affecting the embryonic development were examined.

通过2—细胞期胚胎的显微操作产生了同卵双生小鼠，检验了一些影响胚胎发育的因素。

Orbitotomy latcral and microsurgery were the main treatments for this disorder.

手术是惟一可能根治的手段，外侧眶切开术及显微操作是本病治疗的主要方法。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

During the course of mice ICSI,some critical factors were realized on influencing the experimental results, including micromanipulation systems, diameters of injection pipettes, concentrations of PVP and positions of the first polar body located in oocyte atlCSL

另外，本研究论文还研究了一些对显微注射结果有影响的因素，包括操作液PVP液的浓度、显微注射时卵母细胞第一极体的位置以及显微操作系统等。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新