显微操作的

The paper mainly reviewed several factors on influencing somatic cloning of pig , which involves in cytoplast recipients、 donor cells、micromanipulation、activation and reconstituted embryo transfer .

本文主要综述了影响体细胞克隆猪的几个因素，涉及胞质受体、供核细胞、显微操作、激活以及重组胚移植。

The results of our study have demonstrated that,(1) quadrivanlents in diakinesis Ⅰ are ideal materials for micromanipulation;(2) compared with the instrument used to microisolate chromosomes with a dissecting needle perched on a dissecting microscope outfitting with a micromanipulator or laser beam, the equipment most common used in our study is relatively inexpensive and the techniques could be established as a standard procedure in the ordinary laboratory;(3) the gene mapping methods developed in the study is an efficient and fast approach that could advance gene mapping of fish and other species.

本研究结果表明，（1）减数分裂Ⅰ终变期染色体标本是进行染色体显微操作研究的理想材料；（2）与显微操作仪和微激光分离相比较，本研究所探索的显微分离染色体方法不需要昂贵仪器，在常规实验室即可操作，具有广泛的普及应用意义；（3）把染色体显微操作应用于基因定位这种构思是完全可行的。这种基因定位方法简明而快捷，从而为鱼类及其它物种染色体定位研究开辟了一个新的研究方式和途径。

Objective The origin and the nature of the Hodgkin and Reed-Sternberg cell of Hodgkins lymphoma has been attracting a lot of medical researchers engaged in studying it,for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells.Great improvement has been made since a new method of isolation of single H/RS cells from a frozen section had been set up by KUppers in 1994,and many studies have approved of the B-cell derivation of H/RS cell.lt has been reported that H/R-S cells might partly be originated from B-cell in our research before,but at the same time,we also found that only 18.8% of H/RS cells express CD20,31.3% of immunoglobulin heavy chain rearrangement have been revealed.

目的 霍奇金淋巴瘤(Hodgkins lymphoma，HL)由于它的恶性肿瘤细胞—H／RS细胞一般只占肿瘤组织的极少部分(不到1％)，且散在分布在背景细胞间，因此对于H／RS细胞的来源和性质研究一直是人们探索的目标。1994年德国科隆大学Kūppers等发展了一种从冰冻组织切片上用显微操作仪挑取单个H／RS细胞的显微切割方法进行H／RS细胞基因分析后，人们对H／RS细胞来源的研究有了突破性的进展，多数支持B细胞来源。

Many studies of phosphorus,glucose,hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology,biochemistry,molecular biology and micromanipulation have been reviewed.

对此，人们用形态学、生物化学、分子生物学、显微操作等手段开展了磷酸、葡萄糖、次黄嘌呤和细胞质因素对早期胚胎发育阻滞的影响的研究。

The research aimed to open a new way for raising the magnification of anatomical lens and breaking the limit of micromanipulation.

［目的］为提高解剖镜的放大倍数和打破显微操作的极限开辟新途径。

Furthermore, the prospects of chromosome micromanipulation techniques and FISH will have for future exploration into the fish chromosome research and how to progress genome research of fish are discussed.

此外，还探讨了染色体显微操作和FISH应用于鱼类染色体研究的前景及深入开展鱼类基因组研究的构思。

Using this method, the nucleus was completely eliminated and another was injected using the microscope and micromanipulator. Moreover, the efficiency of nuclear transplantation and survival rate of reconstructed embryos were greatly improved. Furthermore, it is very easy to manipulate and popularize in practice.

实验证明，用此方法可以在只有倒置显微镜和显微操作仪的条件下一次性快速完成去核和注核，大大提高了细胞核移植的效率和重构胚成活率；更重要的是该方法操作简单，新手可以很快掌握该技术，易于在实际工作中推广应用。

This experiment is for the purpose of unifying the porine ovocyte in vitro mature raise and the ROSI correlation operation, establishes in the ovicell nature the round sperm cell microinjection fertilization technology procedure, the exploration porine round sperm cell micro fertilization possibility, the research affects this kind of special cell microinjection fertilization effect the factor, for further studies the porine ROSI technology to provide the basic data.

本试验旨在结合猪卵母细胞体外成熟培养及ROSI相关操作，建立卵胞质内圆形精细胞显微注射受精技术程序，探索猪圆形精细胞显微受精的可能性，研究影响这种特殊细胞显微注射受精效果的因素，为进一步研究猪ROSI技术提供基础资料。

To establish a mouse microinsemination model, Intracytoplasmic sperm injection and subzonal sperm injection were studied use mouse gamets.

我们用小鼠卵细胞胞质内精子注射和透明带下精子注射的方法，对显微授精过程进行了初步探讨，对配子的采集和准备、精子的优化及显微注射的操作等作了阐述。

When the other conditions were stable, the effect of different concentration of the microinjected DNA, and different timing of micromanipulation in CZB medium, different quality of zygotes on microinjection was investigated in vitro. Results: The higher the concentration of the microinjected DNA, the worse the development of zygotes.

获取小鼠受精卵，在其他条件固定的情况下，分别比较不同的注射基因浓度，在CZB操作液中显微操作时间以及注射前受精卵的质量对显微注射后的胚胎体外培养结果的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。