显微技术

METHODS: The lymphocytes exposed to 900 MHz electromagnetic fields with power density of 5 mW/cm2 were measured by confocal Raman microspectroscopy technique.

利用显微激光共焦拉曼光谱技术获得功率密度为5 mW/cm2的900 MHz电磁波照射不同时间的淋巴细胞的拉曼光谱。

This paper presents a new technique of linking ring oven with Fouries transform infrared microspectroscopy to analyse the lead in airborne particles quantitatively.

介绍和讨论了应用傅立叶变换显微红外光谱法与环炉法相结合，定量测定大气飘尘中铅的最新技术。

Aims:Fourier transform infrared microspectroscopy is an advanced bioanalytical technique capable of exploring the chemistry within microstructures of plant and animal tissues at high ultraspatial resolutions without destruction of the intrinsic structures of a tissue.

目标：傅立叶转换红外线显微光谱仪是一种先进的生化分析技术，它能在没有破坏组织内在结构之下，探索在植物和动物组织内微细结构之化学性质，且具有高度的立体空间再现性。

Aims:Fourier transform infrared microspectroscopy is an advanced bioanalytical technique capable of exploring the chemistry within microstructures of plant and animal tissues at high ultraspatial resolutions without destruction of the intrinsic structures of a tissue.

中文摘要目标：傅立叶转换红外线显微光谱仪是一种先进的生化分析技术，它能在没有破坏组织内在结构之下，探索在植物和动物组织内微细结构之化学性质，且具有高度的立体空间再现性。

Results:The experimental results showed that the ultraspatial resolved imagings of plant tissues by FT-IR microspectroscopy with imaging, mapping, ChemiMap, and line scanning techniques were obtained.

结果：实验结果显示藉由傅立叶转换红外线显微光谱仪，运用光学影像、面扫描、化学官能基面像化及线扫描技术，可以获得植物组织立体空间再现性影像。

ABSTRACT PZT thin films with a composition near the morphotropic phase boundary ﹝ w/w=52/48﹞ were prepared by the sol-gel process, and atomic force microscopy and spectroscopic ellipsometry were utilized to trace the sintering processes.

摘 要 用溶胶-凝胶技术制备了组成在准同型相界点﹝m/m=52/48﹞附近的钙钛矿相PZT薄膜，并运用原子力显微分析与椭偏法测试相结合的方法跟踪了薄膜的烧结过程。

Because the myringoplasty is microsurgery so the skilled operation technique and microscope must be necessary for the rate of the success.

提出鼓膜修补术是属显微耳外科手术，要具备熟练的技术并在显微镜下操作，方能提高手术成功率。

Methods The neuroepithelial stem cells were isolated and cultured by microdissection, mechanical blowing and serum-free suspension culture.

方法采用显微解剖、机械吹打、无血清悬浮培养方法分离培养神经上皮干细胞，采用巢蛋白免疫细胞化学染色技术检测神经上皮干细胞，用NSE和GFAP免疫组化染色检测并计数神经细胞和神经胶质细胞。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。