显微技术

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鲤科鱼类Beta-肌动蛋白基因序列，采用长片段PCR技术，克隆了青鱼β-actin基因DNA片段，并构建了青鱼β-actin基因5'启动调控区绿色荧光蛋白表达载体，通过显微注射泥鳅胚胎和COS-7细胞转染实验，证明了所克隆的青鱼β-actin基因5'启动调控区具有启动调控功能，并用之来构建&全鱼&基因的启动子部分。

METHODS:The distribution changes of N CAM at flatfish muscle neuromuscular junction of rat in its embryon phase,14 day after birth,adulthood and 10,20 and 30 days after its L5 lumber nerve root injury were observed by immunohistochemistry and confocal laser scanning microscopy technologies.

采用免疫组织化学方法和激光共聚焦显微扫描技术，观察了大鼠胚胎期，出生后14d，成年期以及L5神经根受压后10，20，和30d，大鼠比目鱼肌神经肌肉接头部N-CAM的分布变化。

Objective To explore the clinical value and technique of CT-guided stereotactic system assisted micro-surgery for epileptogenic cavernous angiomas of brain.

目的探讨利用立体定向技术，辅助皮层电极监测，开放显微手术切除导致顽固性癫痫的脑内海绵状血管瘤，提高微创手术的治疗效果。

Using microanatomic method,the intraorbital and intraethmoidal parts of ethmoidal arteries were observed and measured.

采用显微解剖学技术对60侧成人筛动脉眶内段和窦内段进行观察和测量。

Fibered confocal fluorescence microscopy is a new technique that can be used during a bronchoscopy to analyze the nature of the bronchial mucosa fluorescence microstructure.

纤维共聚焦荧光显微镜技术是一种通过支气管检查法来分析支气管粘膜荧光显微结构性质的新方法。

Rationale: Fibered confocal fluorescence microscopy is a new technique that produces microscopic imaging of a liing tissue through a 1-mm fiberoptic probe that can be introduced into the working channel of the bronchoscope.

原理：纤维共聚焦荧光显微镜这项新技术，是通过将1mm的纤维光学探头插入支气管镜的操作管道，进行活体组织的显微成像

Organelle and biological molecular;the method to show the nucleic acid、protein、enzyme、glucide and lipid;microspectrophotometry and flow cytometry.

胞内核酸、蛋白质、酶、糖类与脂质等的显示方法；显微分光光度测定技术和流式细胞仪

Microstructure and ultrastructure of testes of Haliotis diversicolor was studied by light microscope,scanning electron microscope and transmission electron microscope.

通过光镜、扫描与透射电镜技术对杂色鲍精巢的显微及超微结构进行研究。

It is designed to further the interchange of knowledge and ideas between specialists on different subjects, ranging from the sue of biochemical techniques in herpetological research to electron microscopy, from amphibian and reptilian genetics to the fossil history of the modern amphibians, including systematics and taxonomy as well as behaviour, physiology and ecology.

该杂志的目标是为了使从事不同研究的专家能够交换知识和想法，其内容涉及爬行类研究中从分子技术到电子显微结构水平、从两栖类遗传学到现代爬行类的化石研究的各个方面，同时包括爬行类的系统学、分类学，以及行为学、生理学和生态学等。

Methods Homolateral bone flap with deep iliac circunmflex vessel was transplanted into the defect after clearing up cysts and sequestra of femoral head.

清除坏死股骨头囊肿、死骨后，采用显微外科技术切取同侧带旋髂深血管骨瓣植入股骨头内。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。