显微技术

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

Structure dynamics ofstrongly reduced epitaxial barium titanate (BaTiO3-x) thin films were investigated byMicro-Raman scattering technique, Rutherford backscattering spectrometry, X-raydiffraction, and asymmetric rocking curves.

我们用显微拉曼、卢瑟福背散射谱、X射线散射和非对称摇摆等实验手段研究了在10-2到10-5帕氧气压下用激光分子束外延技术生长的BaTiO3-x薄膜的结构动力学特性。

TiN film is prepared by using multi-arc ion plating technique on the stainless steel substrate. The microhardness is measured by the Hv sclerometer.

采用电弧离子镀技术在不锈钢基片上沉积了TiN薄膜，利用显微硬度计测量了薄膜的表面硬度。

The appearance of Round spermatid injection provides a new way to treat the sterile human male .

圆形精细胞卵胞质内显微注射(Round spermatid injection，ROSI)辅助生殖技术的出现为治疗人类男性不育提供了新的途径。

To clear a sclerotic region around the stapes and to reconstruct ossicular chain and tympanic membrane are the key points of success. The response is closely related with the sclerotic region around the ossicular chain.

尽量清除硬化灶，形成宽大的鼓室，并重建鼓膜和听骨链，手术疗效与病灶影响听骨链程度有关，精确、熟练的耳显微外科技术是成功的关键。

Methods Fluorescence in situ hybridization with a whole chromosome 7 painting probe and a 7q subterminal probe (7q36→qter), prepared by chromosome microdissection technique, was used to analyze a case with a history of spontaneous abortion and 7q terminal deletion detected by conventional G-banding technique.

显微切割制备7号和7q亚端粒(7q36→qter)探针，通过荧光原位杂交技术分析一例具有反复流产史且常规G带发现有7q末端缺失的病例。

By adding ZrO2(4Y) to the thermit,the influences of centrifugal force and eutectic composition on the microstructure,mechanical properties, strengthening and toughening of ceramics lining are researched.

基于燃烧合成理论，在铝热剂中添加ZrO2(4Y)粉末，采用离心技术制备出了高强度、高韧性的Al2O3/YSZ共晶系陶瓷内衬复合管，并研究了离心力、共晶成分对陶瓷内衬显微结构、力学性能及强韧化本质的影响。

A transmission grating with an area of 1 mm × 1 mm, a pitch of 300 nm, and a gold thickness of 1 μm for X-ray imaging and microscopy is successfully fabricated by combining electron beam lithography, X-ray lithography, and electroplating.

利用电子束光刻、X射线光刻和微电镀技术，成功制作了面积为1 mm×1 mm，周期为300 nm，金吸收体厚度为1 μm的用于X射线显微成像的透射光栅。

A series of indexes reflecting free radical changes, anti-oxidations, Immune function, endocrine function was studied by Chemiluminescence method, immunoturbidimetry, ANAE dye method, micro-hemagglutination inhibition test and radio immunoassay in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially. The pathological changes including microscopic and ultramicroscopic changes in the relative organs and tissues were observed.

本实验以一日龄150只海兰兰蛋鸡为实验动物，用低维生素E且添加鱼油日粮饲喂雏鸡，复制脑软化症动物模型，采用化学发光法、免疫浊度测定法、酸性α-醋酸萘脂酶染色法、微量血凝抑制试验、放射免疫测定技术等方法系统地检测了脑软化雏鸡的自由基代谢和抗氧化功能、红细胞免疫功能、细胞免疫功能、体液免疫功能及非特异性免疫功能等指标，并对患雏病理组织进行了显微及超微结构观察。

Methods The otoconia of 18 guinea pigs were damaged by a high G force (10 Gy 5 min), and their utricle macula were observed by scanning electron microscopy at 1 h, 2 d and 8 d after the damage respectively. The elements of different kinds of otoconia were measured qualitatively and quantitatively by electron probe Xray microanalysis.

给予过载刺激(10 Gy 5 min)造成18只豚鼠耳石的损伤破坏，用扫描电镜（scanning electron microscopy，SEM）分别观察过载刺激后1 h、2 d、8 d时其椭圆囊囊斑，用X射线显微能谱分析技术(electron probe Xray microanalysis，EPMA)测量不同形态类别的耳石元素构成。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。