显微技术

The main techniques in Nanobiology are scanning probe microscope and Optical Tweezer.

纳米生物学研究的技术手段目前主要是扫描探针显微术(Scanning Probe Microscope，SPM)和光钳技术。

Methods Autometallography, immuno-electron microscopy and confoeal laser scanning microscopy were used to investigate the colocalization of zinc ion, zinc transporter ZnT3 and glutarnic acid decarboxylate.

方法应用锌金属自显影技术、免疫电镜技术和共聚焦激光扫描显微术，研究游离锌离子、锌转运蛋白（zinc transporter 3，ZnT3）与（glutamic acid decarboxylate，GAD）在小鼠脊髓内的共存情况。

It is very detrimental to materials performance. From the viewpoint of the effects of PPB precipitation on the frature of hipped P/M Rene'95, the surface segregation and reaction in Rene'95 powder metallurgy superalloy were analyzed by modern surface techniques and by electron microscopy. The mechanisms of the formation and reduction of PPB precipitation are revealed.

本文从原始颗粒边界析出对热等静压压坯断裂的作用的角度出发，利用现代表面分析技术及电子显微分析技术研究了Rene′95雾化粉末表面的偏析、预处理粉末的表面反应和表面析出相的粗化规律以及热等静压压坯的界面反应和原始颗粒边界析出相，揭示了原始颗粒边界析出的形成机理和预处理方法改善原始颗粒边界析出的原因。

This study is to investigate strategies, techniques and other factors in the microneurosurgcial management of intramedullary spinal ependymomas.

随着神经影像技术，显微外科技术和术中监测手段的发展，大多数脊髓髓内肿瘤的早期诊断和治疗已成为现实。

The ultrathin cryosections and ethanolic phospho tungstic acid staining sections of rat atrial cardiocytes were prepared. Quantit ative electron probe Xray microanalysis of Hall continuum method was adopted to measure the alteration of Ca content in ASG at the exact time intervals of 15, 30, 60, 120 min after the adrenaline administration.

冷冻超薄切片技术和磷钨酸-乙醇特殊染色技术制备大鼠心房切片。X射线显微定量分析采用Hall连续谱法，用以测量在肾上腺素处理后15、30、60和120 min时ASG中钙含量的变化。

Intracytoplasmic sperm injection is a very important assistant reproductive technology for treatment of man infertility and fundamental research , Success in ICSI in human and other animals has recently been achieved., The present pilot studies were conducted to establish a preliminary procedure for ICSI in mice, explore the experimental conditions of ICSI in mice, investigate factors influencing the test results, and accumulate technologic basis for the next studies on pronuclear exchange in mice .

本部分研究论文初步建立了小鼠ICSI技术的基本操作程序，摸索了小鼠ICSI研究的操作条件，总结了一些影响显微注射效果的重要因素，并为下一部分小鼠原核置换的研究奠定了雄厚的技术基础。

To investigate the possibility of such a transition, we studied 17 cases of MGA or atypical MGA some of which had given rise to carcinoma in situ and/or invasive ductal carcinoma using the reticulin stain, immunohistochemistry (S-100, p63, Ki-67, and p53), and a molecular approach involving microdissection and high-resolution comparative genomic hybridization and MYC chromogenic in situ hybridization.

为了研究这种转变的可能性，我们分析了17例典型或非典型的MGA，其中一些已经发展成为原位癌和/或浸润性导管癌，所用的方法是网状染色、免疫组化（S-100， p63， Ki-67， and p53）和分子技术，分子技术包括显微切割和高分辨率比较基因组杂交及MYC显色的原位杂交。

Methods:Laser capture microdissection was used to separate human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells, Proteome alterations were observed using two dimensional polyacrylamide gel electrophoresis(2-DE) technique and electrospray ionisation tandem mass spectrometry.

通过激光捕获显微切割技术分离ESCC肿瘤细胞和癌旁上皮细胞，通过双向电泳和质谱技术鉴定表达异常的蛋白，并通过蛋白免疫印记证实部分差异蛋白的表达。

Firstly, the different effect of rectorite modification for the mechanical property of E-51 system were compared; the effect of organic clay for the viscosity and Gel-time of pure E-51 epoxy resin were checked separately; the influence of organic clay for hardening process was studied with FTIR technology; And, the infection of organic rectorite content in matrix for the mechanical property thermal-wet tolerance property aging resistance property and medium endure property for the both of two system were studied relatively. The effect of organization for the inter-layer distance of clay was denoted by XRD technology; To probe into the thermodynamics status about reaction, we investigated the heat discharging of reaction system in the help of DSC. The impact and crooking of solidifying system was observed with the SEM to investigate the decentralization of organic clay, and, at the base of this ,we discuss the mechanism of clay toughing-buildup polymer matrix.

分别考察了有机粘土对树脂体系粘度和凝胶特性的影响，利用红外光谱分析技术研究了有机粘土的加入对体系固化工艺的影响；分别考察了有机粘土含量对二体系的力学性能、耐湿热性能、耐老化性能以及耐介质性能等的影响；采用X-衍射实验表征复合材料中有机粘土层间距的变化；用视差扫描量热法考察了体系的放热情况，以探讨反应的有关热力学状况；通过扫描电子显微分析技术对浇注体的弯曲、冲击断口进行观察分析，以考察、表征有机粘土的分散情况，并在此基础上探讨了有机粘土改性环氧树脂的机理。

The software of the system was integratively programed by C++ language.

该系统运用显微图像技术和BP神经网络技术对试验样本的细菌总数进行分析和计数。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。