显微技术

And thaw, and cell fusion; Understanding the principles and operation procedures for autoradiograph; Mastering the skills of writing experiment reports and graphic drawing under microscope.

本课程是农业与生物学院本科学生的一门实验课程，本课程主要内容包括：掌握和了解普通光学显微镜、几种特殊显微镜和电镜的一般原理和使用方法；掌握显微镜临时标本的制备，会进行显微测量、细胞计数和染色体观察；初步观察细胞生理现象、细胞化学成分显示、染色体标本制备；掌握细胞培养、细胞冻存与复苏、细胞融合等技术的原理和方法；了解细胞放射自显影技术的原理和操作步骤；掌握实验报告的书写和镜下绘图的技能。

Methods (1)We observed the serial sheet sections of the optic apex region obtained using collodion microtomy in 4 adult cadaver heads;(2)High resolution CT scanning and three dimensional images of the optic apex region were observed and measured in 10 adult volunteers;(3)High resolution magnetic resonance images of the optic apex region were obse.

①采用火棉胶切片技术对4例(8侧)成人眶尖区标本进行连续薄层断层解剖学观察；②使用高分辨力CT及三维重建技术观测10例(20侧)志愿者眶尖区；③观测5例(10侧)志愿者眶尖区高分辨力MRI断层扫描图像；④对30例(60侧)成人干颅骨标本和15例(30侧)成人湿性头颅标本的眶尖区结构进行显微解剖；⑤在手术显微镜和内窥镜下对3例成人头颅湿性标本模拟眶内经颅入路。

With the development of biotechnology, the technology of microinjection has been widely applied in the area of cell manipulation.

随着生物技术的发展，显微注射技术在细胞操作方面得到了广泛的应用。

Laser microdissection provides a rapid isolating and purification modern method for biological tissues and cellular preparations.

激光显微切割技术是20世纪90年代后期发展，由现代物理学和生物学相结合的精确取材技术。

In this paper, the applied research of cell microinjection is based on the micro-fluidic digital technology above.

本论文是上述微流体数字化技术在细胞显微注射技术上的实验研究。

Unlike current diffuse reflectance visible spectroscopy, the visible microspectroscopy can obtain the cell-level spectral information, It is not affected by changes of optical path length induced by different set-up of prober and hemoglobin concentration due to the pressure between prober and tissue.

对比常见的漫反射可见光谱技术，可见光显微光谱技术能够获取细胞大小范围内的信息，而且不受探头结构差异导致的传输光程长短，以及测量时探头施加压力导致的血红蛋白浓度异常变化的影响。

In order to solve the problems of sampling technology in spraying, a new measurement method of using micrograph combining with digital image processing technology for spraying deposition parameters analysis and sample in airtight space was given.

针对植保机械喷雾取样技术存在的不足，提出了利用显微图像结合数字图像处理技术对密闭空间中雾滴的沉积状态参数进行取样和分析的方法。

The influence of low-frequency electromagnetic field on microstructure and morphology of primary Si particles of Al-19.2% Si alloy ingot specimens prepared by semicontinuous casting process was investigated with electronic optical microscopy, where the specimens were taken from different parts of the cast to which different electromagnetic fields are applied.

采用低频电磁半连续铸造技术制备Al-19.2% Si合金铸锭，利用电子光学显微镜对铸锭的显微组织进行分析比较，研究了电磁场强度和频率对初生硅颗粒形貌的影响；利用测温技术对合金的凝固过程进行监测，研究低频电磁场对凝固前沿温度场的影响。

The embryological studies of strawberry showed that the ovule of strawberry were hemitropous, the ovule had the obvious hypostace structure, the type of its embryo sac was Polygonum, the embryo sac was long and narrow, the type of development of embryo was the Geum type of the Asterad type, the endosperm was Nuclear type and the somatic embryo sacs happened The integral transparency technique combined with the technique of microdissection was used in this experiment The combination was an innovation in the techniques of section.

对草莓胚胎学的研究结果表明：草莓胚珠横生、胚珠具明显的承珠盘结构、胚囊发育为蓼型、胚囊狭长、胚胎发育为紫菀型水杨梅类型、胚乳核型、并有体细胞胚囊的产生。试验中采用了多种制片方法，其中将整体透明技术和显微解剖技术相结合的方法是在制片技术上的一次创新。

College of Life Science, Wuhan University, Wuhan 430072On the basis of optimization of in situ RNA hybridization technique, Westudied the temporal and spatial expression of calmodulin and phosphorylasemRNA and its relation with fertilization and embryogenesis in rice as well as antherdevelopment in tabacco. We also constructed cDNA libraries of proembryos anddifferentiating young embryos in rice using microscopic isolation and RT-PCRtechniques.

本文在优化RNA原位杂交技术的基础上，研究了钙调素mRNA和磷酸化酶mRNA时空表达及其与水稻受精和胚胎发生以及烟草花药发育的关系；利用显微分离技术和RT-PCR技术建立了水稻原胚和分化幼胚cDNA文库。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。