显两色

Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans. Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common Pathogenic non-Candida albicans (Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis), and the products were digested with the two restriction endonucleases (Msp Ⅰ and Hae Ⅲ) respectively. Results The four isolates consisted of 15 strains of Candida glabrata (7.50%), 7 strains of Candida parapsilosis (3.50%), 5 strains of Candida krusei (2.50%), 2 strains of Candida tropicalis (1.00%).

首先采用芽管试验、厚壁孢子试验、法国科玛嘉念珠菌显色培养基及API20CAUX酵母菌鉴定系统将分离自VVC患者阴道内的念珠菌菌株鉴定到种，然后采用真菌通用引物将4种常见非白念珠菌（包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌）进行PCR扩增，并选用MspⅠ和HaeⅢ两种内切酶对扩增产物进行酶切分析。

By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面，本文在优化并确定了多基因PCR反应条件的基础上，采用了两种方法对多基因PCR产物进行鉴定：①采用共价结合方法将多个探针固定在Biodyne〓C膜上，将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应，首次建立了GMO的多基因PCR-膜相杂交检测方法，实现了一个反应体系中同时检测多种转基因成分的目标，提高了检测效率与准确率。

Before the two-hour interaction of male-male individuals , we found there were obviously fewer and lighter stained ERp immunoreactive neurons (ER|3-IRs) in both main olfactory bulb system project regions and vomeronasal system project regions in the mandarin voles than in the reed voles, but no significant difference in numbers of AR immunoreactive neurons between the two species through the examining of immunoreactive neurons, moreover, there were significantly fewer AR-IRs than ERp-IRs in two species of vole before the two-hour interactions.

免疫组织化学检测，没有社会交往时棕色田鼠主嗅球系统投射区和犁鼻系统投射区ERβ免疫阳性细胞明显少于沼泽田鼠，且显色淡，AR免疫阳性细胞在两种鼠间差异不大，且都明显少于各自的ERβ-IRs.2h交往后，棕色田鼠主嗅球投射区和犁鼻系统投射区的ERβ-IRs细胞数明显少于交往前，AR-IRs细胞数明显多于交往前；沼泽田鼠交往前与交往后ERβ-IRs和AR-IRs细胞数均无显著差异，且显著多于交往后棕色田鼠ERβ-IRs细胞数，显著少于交往后棕色田鼠AR-IRs细胞数。

In the two actual tunnel, we measured and calculated visibility of the small target under the three light source, and made a visual experimental method of the small target color.

在两条实际隧道中对三种光源照明下的小目标可见度进行了实际的测量和计算，并且提出了小目标显色性的视觉实验方法。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。