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SSCP was detected in exon 6 and exon9p of the gene, but not in the other exons.Results In polyacrylamide gel electrophoresis, We have not found abnormal conformer in both affected individuals and control cases. There were no differences in the DNA sequence between these two groups.

结果 在非变性聚丙烯酰氨凝胶电泳中，未发现DCM组和对照组该基因的外显子6和外显子9p出现异常构象，通过DNA测序证实两组的核苷酸序列没有差异。

By the case of skewed cavity flow with 30-degree angle, the convergence performance of SIMPLE/SIMPLEC algorithm was analyzed on unstructured grids and the influence of accepting or rejecting non-orthogonal terms was compared.

利用30°角的斜方腔流动计算成果，分析了非结构同位网格的SIMPLE/SIMPLEC算法的收敛性能；比较了因网格的非正交而引入的非正交项的取舍对该算法收敛性能的影响；并采用显式校正步法对SIMPLEC算法进行了显式校正。

The prolactin gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Single nucleotide polymorphisms of exon 1 to exon 5 of PRL gene were detected in high fecundity breeds and low fecundity breeds (Dorset, Texel and Corriedale sheep) with five pairs of primers by PCR-SSCP.

设计5对引物，采用PCR-SSCP技术检测催乳素（prolactin，PRL）基因外显子1至外显子5在高繁殖力绵羊品种和低繁殖力绵羊品种（多赛特、特克塞尔和考力代）中的单核苷酸多态性，同时研究该基因对小尾寒羊高繁殖力的影响。

Polymorphism was detected for exon 3 of BMP4 gene in four sheep breeds. For exon 3, three genotypes were detected in four sheep breeds. In Small Tail Han, Chinese Merino, Corriedale and South African Mutton Merino sheep, frequency of genotype AA was 0.017, 0.216, 0.115, 0.429, frequency of genotype AB was 0.102, 0.317, 0.269, 0.500, frequency of genotype BB was 0.881, 0.467, 0.616, 0.071, respectively. Sequencing revealed one singlenucleotide mutation C→A at 305 bp of exon 3 of BMP4 gene in genotype BB in comparison to genotype AA, and this mutation resulted in an amino acid change of alanine→aspartic acid.

对于外显子3的引物，在4个绵羊品种中都检测到3种基因型；统计结果表明：小尾寒羊、中国美利奴绵羊、考力代绵羊、南非肉用美利奴绵羊AA基因型频率分别为0.017、0.216、0.115、0.429，AB基因型频率分别为0.102、0.317、0.269、0.500，BB基因型频率分别为0.881、0.467、0.616、0.071；测序结果表明：BB型和AA型相比在BMP4基因外显子3第305位碱基处发生C→A的突变，该突变导致氨基酸由丙氨酸改变为天冬氨酸；BB型小尾寒羊平均产羔数分别比AB型和AA型多0.61只（P.05）和1.01只（P.05）。

These analyses disclose the potential components influencing the exon splicing frequency, and provide the clue for further decipherment of novel ASEs creation, splicing selection and transcription regulation.

这些分析结果揭示了影响外显子剪接频率的潜在组成特征，也为进一步解读新的选择性剪接外显子的生成、剪接选择和转录调控提供了线索。

Methods The learning and memory capability of mice was investigated by Y-water maze test and step-down test. The contents of protein, DNA and RNA were investigated by Lowry methods, the diphenylamine reagent method and Oricinol reagent method.

用Y-型水迷宫法、跳台法测定小鼠的学习记忆能力，Lowry法测定小鼠脑内蛋白质，二苯胺试剂显色法测定DNA，Oricinol试剂显色法测定RNA的含量。

In this report, the 59Tc labelling of Annexin V fragment modified with HYNIC (HYNIC-Anx13) using Tricine, EDDA or EDDA/Tricine as coligands was described. The effect of various factors on 99Tc labelling was investigated. Biodistribution studies in normal mice and gamma imaging of apoptosis in rats induced by cyclophosphamide were performed.

为探讨99Tc标记的HYNIC-Anx13用于细胞凋亡显像的可能性，分别以N-［三甲基］甘氨酸，乙二胺－N，N'-二乙酸和EDDA/Tricine为协同配体，对经6-肼基烟酰基修饰的膜联蛋白V片段(HYNIC-Anx13)的99Tc标记条件和主要影响因素进行了研究，并完成了HYNIC-Anx13的99Tc标记物在正常小鼠体内的生物分布和大鼠细胞凋亡模型的显像实验。

You are an emotionalism person, sometimes reveals in a lively way active, is likely in crowd's focal point, sometimes reveals lonely, a person places outside the crowd.

你是一个情绪化的人，有时显的活泼好动，象是人群中的焦点，有时显的孤独，一个人身处人群外。

Based on the classic composites laminates theory,the stiffness matrix and the equivalent damping matrix of the laminates which can be described by elastic constants and damping constants were determined.

根据经典层板理论分别推导出了显含弹性常数和显含阻尼系数的纤维增强复合材料层板的刚度矩阵和等效阻尼矩阵。

First production of 10,000 tons annual capacity reagent resin, 5,000 tons of activated reagent, cationic etherification agents and oil additives several major series, a number of brand products.

先生产年产10000吨树脂显色剂、5000吨活性白土显色剂、阳离子醚化剂和油品添加剂几大系列、多个品牌的产品。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。