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The results showed that taurine has no significant influence on testis index, epididymis index, spermaduct index, penis index and testis tectology of baby and young rats, but can make old rat testis tectology better, prevent convoluted seminiferous tubule degeneration, germ cell distortion and leydig cell atrophy. Taurine increase the level of T, E2 and LH in baby rat obviously, but has no significant effect on the level of FSH. The level of T and LH was obviously increased by taurine in young and old rats, but the level of E2 and FSH has no significant changes.

结果证明:牛磺酸对幼年、青年、老年大鼠睾丸指数、附睾指数、输精管指数和阴茎指数没有明显影响;对幼年、青年大鼠睾丸组织形态没有影响,但可明显改善老年大鼠睾丸组织形态,阻止曲细精管退化、生殖细胞变形和间质细胞萎缩;显著促进幼年大鼠 T 、 E2、 LH 分泌,而对 FSH 没有明显影响;明显促进青年和老年大鼠 T 和 LH 分泌,而对 E2和 FSH 没有明显影响。

Result: Immunohistochemic results indicate that after HBO treatment, the expression of CytC and Bax in the neuronal cytoplasm decline significantly ( P.05) except in the time of 72 hours after TBI; the Bcl-2 expression is up-regulated significantly(p.05) after TBI. The images of electromicroscope indicate that before 24hr after TBI, mitochondria of control groups become more swoln even fracted and the matrixes become vaguer than those of being treated with HBO.

郑州大学2004年硕士研究生毕要论文高压氧对大民实脸性脑顶伤后神经细胞内cytochr她eC,Bol一2以及Bax表达的影响结果:(1)对于致伤后的脑组织切片染色发现,相同时间段内(3,6,12,24小时)实验组神经细胞内CytC阳性表达明显低于对照组(P.05),而72小时以后无明显差异;Bel一2阳性表达于3,6,12,24,72小时时明显高于对照组(P.05),Bax阳性表达于3,6,12,24小时时明显低于对照组,72小时时无明显差异。

12H treatment group compared with 12h model group: the density of light of HSP70 of the Gardenoside group is not different and which of it of TGFβ〓 increased with the model group; the density of light of the HSP70 of the Baicalin group in not different and which of the TGF-β〓 is no different with the model group, the density of HSP70 of the Concha Margatitifera Usta is not different and which of TGFβ〓 increased than the model group; the density of the HSP70 of the Cholic Acid group is no different and the density of TGF-β〓 increased than the model; the density of the light of HSP70 of the Hefang Group increased and which of TGF-β〓 increased than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组明显升高(p<0.01)。大鼠局灶性脑缺血24小时模型组缺血脑组织TGF-β1光密度值含量较正常组明显增高(p<0.01)。珍珠母治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组明显升高(p<0.01)。胆酸治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组明显升高(p<0.01)。栀子苷治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组无显著性差异(p>0.05)。黄芩苷治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组明显升高(p<0.01)。

RESULTS:In the serum,SOD activity of all the nm-haFGF groups and the haFGF group increased significantly while the content of MDA decreased dramatically compared with the model group;The content of BUN and Cr aland haFGF group rose significantly compared with the model group,while MDA decreased dramatically.

结果:缺血再灌注24 h后,nm-haFGF所有剂量组和haFGF组血清SOD活性明显高于模型组,MDA含量明显低于模型组,而血清和尿BUN和Cr含量均明显低于模型组;肾组织SOD活性在nm-haFGF 20 ìg/kg和40 ìg/kg剂量组和haFGF组明显升高而MDA含量明显降低。

The levels of SOD and CAT significantly increased and MDA decreased in bifidobacterium group compared to radition damage group.Electronic microgragh of spine neurocyte and neurogliocyte showed that cytoplasm,cytoblast and plastiosome were significantly engorged ,and neurogliocyte apoptosised in earlier period. TLR2mRNA increased in C group compared to B group by RT-PCR. Cytochrome C in kidney increased in C group compared to B group, but bcl-2,NF-Кb decreased. Conclusions : Enteroclysm is better compared to intragastric administration.

双歧杆菌处理组肝组织中SOD和CAT水平明显高于放射损伤组(P.01),同时MDA明显低于放射损伤组(P.01);电镜结果显示:放射损伤组与双歧处理组的细胞浆、细胞核和线粒体均明显肿胀,双歧处理组同时具有早期凋亡现象;RT-PCR结果显示:双歧处理组TLR2mRNA的表达明显高于放射损伤组;免疫组化结果显示,双歧处理组Cytochrome C的表达明显优于放射损伤组,而NF-кBp65和bcl-2则相反。

Results:(1) CBC possesses a natural trabecular structure. Its components is similar to the mineral of bone.(2) CBC has great cellur biocompatibility. It can promote cell adhesion, proliferation and secretory while needn t modification.(3) In nude mice, the ectopic osteogenesis of MCCAB was obvious which was better than that of CBC alone and the new bone formation increased in pace with time. In the MCCAB, both intramembranous and cartilaginous osteogeneses were seen but the former dominated.(4) When MCCAB was autotransplanted intramuscularly, the osteogenesis was much better than that of negative control groups.(5) The MCCAB can repair autogenous calvaria defects effectively and the effect was much better than that of CBM alone and blank group. Conclusion:(1) CBC possesses good biocompatibility, osteoconduction and porous structure, can be used as scaffold material for constructing of tissue engineering bone.

结果:(1)CBC具有天然的多孔网状结构,最大程度地保持了骨矿的微结构特征,可以作为骨组织工程的支架材料;(2)CBC细胞亲和性好,不需作修饰即能促进成骨细胞的粘附、增殖和分泌活动;(3)MSO/CBC复合人工骨在裸鼠皮下异位成骨作用明显,兼有膜内成骨和软骨内成骨的特点,成骨效果明显优于单纯CBC植入组;(4)MSO/CBC复合人工骨兔自体肌袋异位成骨作用明显,血管化充分,成骨效果明显优于单纯CBC植入组;(5)MSO/CBC复合人工骨修复兔颅骨临界性缺损效果好,明显优于单纯植入CBC组和空白对第四旱区大学硕士学位论文一照组,可以作为骨移植替代材料用于骨缺损畸形修复。

We didn\'t find activated points in cerebellum and deeper brain.3.Acupuncture of sham point can significantly activated BA2,6,8,13,21,37,40,44,45,47,putamen and other areas.Both the left and right side of the brain have activated points,but points on the right side are more than those on the left.Both sides of middle temporal gyms,parietal lobule,supramarginal gyms and the lentiform nucleus have obvious activated points.The activated points mainly concentrated in the cerebral cortex,the deep-activated are mainly in the putamen.4. Sham needling in sham point can significantly activate BA6, 8,9,10,18,21,37,40,43,44.The activated points are mainly distributed in the right side of the brain.The left side also has some activated points;5.In the comparasion of Acupunture and sham-needling in S J5,we find that BA8 and cerebellum have distinct regional activated points;6.In the comparasion of acupuncture in SJ5 and sham point,we find BA2 and left cerebellar regions have activated points;7.Sham needling in S J5 compared with sham point,we find BA7,8,9,18 and other areas have activated points,the main activated points are at the left brain.It is not difficult to find that the distribution of activated points are mainly in the middle brain,no obvious activated points at the temporal lobe.

结果:通过对数据的处理和分析,我们初步发现:1、外关穴真针刺能显著激活Brodmann area45、37、44、40、22、13、37、47区、海马、杏仁核、黑质等区域,小脑左侧更明显,左侧颞叶皮层激活点多于右侧,且脑部左侧深层激活点多于右侧;2、外关穴假针刺能显著激活BA46、44、41、13、40、37、19区等区域,激活点主要集中表现在大脑皮层,以颞叶为主,小脑及深部未发现明显激活点;3、非穴真针刺组结果分析初步表明,BA2、6、8、13、21、37、40、44、45、47区以及壳核等区域有激活点,大脑左、右侧均有激活点,但右侧更多,双侧颞中回、顶叶下小叶、缘上回及豆状核有明显激活点,激活点主要集中在大脑皮层,深部激活点主要在壳核;4、非穴假针刺能显著激活BA6、8、9、10、18、21、37、40、43、44区等区域有激活点,主要分布在大脑的右侧,左侧也有不少激活点,就其具体分布主要在颞叶和额叶,少部分分布在顶叶和枕叶;5、外关穴真针刺与假针刺对比发现,BA8区和小脑等区域有明显的激活点;6、外关穴真针刺与非穴真针刺对比发现BA2区、左侧小脑等区域有激活点;7、外关穴假针刺与非穴假针刺对比发现,BA7、8、9、18等区有激活点,主要反应点在左侧大脑的枕叶中回、楔叶,顶叶楔前叶及额上回、中回,就其分布不难看出主要在大脑中部,颞叶未见明显激活点。

Results: In acute experiment, there was no significant difference of body weight or organ indexes between the normal control and arsenic poisoning groups ; while the CH50 level, lymphocyte accruing rate, phagocytosis rate and macrophagocyte index in arsenic poisoning group were significantly lower (P.05 or P.01) than those in normal group. There was no significant difference of body weight or organ index between arsenic poisoning group and EXD (10 g) treated arsenic poisoning group, while CH50, lymphocyte accruing rate, phagocytosis rate, and mavrophocyte index were significantly higher in EXD (10 g) treated arsenic poisoning group than those in arsenic poisoning group (P.05 or P.01). In EXD (5 g) treated arsenic poisoning group, results of all the tested indexes were the same as those of arsenic poisoning group.

结果:与正常对照组相比,砷中毒组小鼠体重、脏器指数无明显改变;CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数明显降低,差异有显著性(P.05或P.01;与砷中毒组相比,竹荪治疗低剂量组小鼠体重、脏器指数、CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数无明显改变化;竹荪治疗高剂量组除小鼠体重、脏器指数无明显改变外,CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数明显升高,差异有显著性(P.05或P.01)。

At 4-week, GAG content in JG as significantly lower than that in RG and CG, while dead cell ratio in RG significantly higher than those in JG and CG. Mankin scores in RG and JG ere significantly higher than that in CG. At 8-week, GAG content in RG as significantly lower than that in CG, while dead cell ratio and Mankin scores ere significantly higher than those in CG.

结果 显微观察显示两组训练动物在训练4周时便开始有膝关节软骨过劳性损伤早期改变,8周时损伤改变进一步加重。4周时,蹦跳组GAG含量明显低于对照组及跑步组,跑步组死亡细胞率明显高于对照组以及蹦跳组,跑步组和蹦跳组Mankin评分明显高于对照组;8周时,跑步组GAG含量明显低于对照组,死亡细胞率与Mankin评分明显高于对照组。

Had rarely or never used protective;(11) MiceGrayAuto had realized the automatic processing of video data from the sexual preference observation system of rats, and MiceGrayManu had accomplished the analysis of images of sexual behaviors of rats;(12) Peripheral given of high dose androgen to female rats before its brain sexual differentiation had caused masculinization and defemininization in female rats, therefore led to homosexuality, and based on the results, we had established the female rat homosexual model;(13) Peripheral given of estrogen to male rats before its brain sexual differentiation had little effects on the sexual preference and sexual behavior of adult rats;(14) Peripheral given of estrogen antagonist to male rats before its brain sexual differentiation had markedly reduced masculine sexual behavior and interfered male sexual preference, but had no effect on feminine sexual behavior, and based on such results, we had established the male rat homosexual model, however the model have to be improved for better sensitivity;(15) The volume of sexually dimorphism nucleus of the preoptic area of the high dose androgen-treated female rats was significantly greater, while on the other hand the volume of AVPV of which was significantly lesser, than the female rat control.

并且有17.79%的男性同性恋者在1年内与异性保持着性关系。但在与异性阴道性交时,绝大多数(92.51%)偶然或不使用安全套;(11)MiceGrayAuto软件实现了大鼠性偏爱视频数据自动处理功能,MiceGrayManu软件实验了大鼠性行为图象分析功能;(12)大脑性分化前高剂量的雄激素有促雌鼠雄性化和去雌性化的作用,并表现为同性性倾向,建立了雌鼠同性恋模型;(13)在雄鼠大脑性分化前外周给予雌激素对成年后的性偏爱和性行为未产生明显影响;(14)在雄鼠大脑性分化前外周给予雌激素拮抗剂明显削弱雄性性行为,阻碍了雄性性偏爱,但雌性行为无明显变化,雄鼠的同性恋模型还有待进一步调整和改进:(15)高剂量雄激素处理的雌鼠SDN-POA体积明显大于对照雌鼠组,而AVPV体积明显小于对照雌鼠组。

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