明显

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组：早期RA家兔模型15只，品种及体重接近。对照组：正常家兔10只，品种及体重接近。RA家兔模型制作方法：选取15只成年家兔，体重、品种接近，将卵蛋白溶解于生理盐水，配成浓度为20mg/ml的溶液，与等量完全弗氏佐剂混匀，注入家兔肩胛区皮下致敏，每只家兔每次注射1ml，于肩胛区5个不同区域注射，每周一次，连续注射3周而致敏，第4周向膝关节腔注射卵蛋白生理盐水溶液，每只关节腔注射5mg卵蛋白，15只家兔膝关节全部注射，24h内此关节直径和表面温度大幅度上升，以后缓慢下降，至14～21d达到平台期，发病率达100%。造模成功后第1～4周为早期改变，第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组，其股骨髁软骨的厚度与对照组相比无明显差异，其软骨下骨皮质与对照组相比无明显改变。

The shorter internodes and out-growth of anxillary buds found in antisense LeETR2 and leaf epinasty observed in antisense LeETRl plants are phenotypes consistent with the negative regulation model of ethylene.

5植株衰老和叶片脱落延迟，花瓣脱落也明显的延迟，在转反义LeETR1基因番茄的果实成熟特性被明显改变，表现在乙烯释放高峰延迟，果实颜色出现变异，以及和成熟相关的酶活性的改变，然而，转反义LeETR2基因的果实的成熟特性与对照相比无明显差别。

Results Compared with control group, the wound area and wound volume at different day in rhaFGF group were significantly diminutedP＜0.05）, the healing rate was predominantly eleva Ted (P＜0.05）, the healing time was significantly shortenedP＜0.05）. RhaFGF resulted in a remarkable enhancement of granulation tissue growth and epithelization in wound.

结果：与生理盐水对照组比较，rhaFGF组伤后各时间点的创面面积、伤腔容积均明显缩小﹙P＜0.05﹚，愈合率显著提高﹙P＜0.05﹚，创面愈合时间明显缩短﹙P＜0.05﹚。rhaFGF组的肉芽组织生长及再上皮化明显加速。

Rabbit anti-thrombosis in oral fungus polysaccharides 18.5mg/kg, can significantly extend the specificity of thrombus and fibrin thrombus formation time and shorten the length of thrombus, reduce the thrombus wet weight and dry weight, reduce platelet count, lower platelet adhesion rate and blood viscosity, and can significantly shorten the guinea pig euglobulin lysis time, lower plasma fibrinogen levels, elevated plasmin activity, results showed that the fungus polysaccharides significantly antithrombotic effect.

抗血栓形成兔口服木耳多糖18.5mg/kg，可明显延长特异性血栓及纤维蛋白血栓的形成时间，缩短血栓长度，减轻血栓湿重和干重，减少血小板数，降低血小板粘附率和血液粘度，并可明显缩短豚鼠优球蛋白溶解时间，降低血浆纤维蛋白原含量，升高纤溶酶活性，结果表明，木耳多糖有明显的抗血栓作用。

Rabbit anti-thrombosis in oral fungus polysaccharides 18.5mg/kg, can significantly extend the specificity of thrombus and fibrin thrombus formation time and shorten the length of thrombus, reduce the thrombus wet weight and dry weight, reduce platelet count, lower platelet adhesion rateand blood viscosity, and can significantly shorten the guinea pig euglobulin lysis time, lower plasma fibrinogen levels, elevated plasmin activity, results showed that the fungus polysaccharides significantly antithrombotic effect.

抗血栓形成兔口服木耳多糖18.5mg/kg，可明显延长特异性血栓及纤维蛋白血栓的形成时间，缩短血栓长度，减轻血栓湿重和干重，减少血小板数，降低血小板粘附率和血液粘度，并可明显缩短豚鼠优球蛋白溶解时间，降低血浆纤维蛋白原含量，升高纤溶酶活性，结果表明，木耳多糖有明显的抗血栓作用。

Positive self conscious emotion cognition develops fast from grade 2 to grade 4. Passive self conscious emotion cognition develops slower than the positive one.5 Primary school children"s knowledge about the reason of emotion and knowledge about behaviors of emotion develop fast with the age increased at primary school period.6 Pupils" emotion cognition to the emotion expresser is better than that to the emotion recipient apparently.

积极自我意识情绪认知在2～4年级间有明显发展，发生了"能与不能"质的转变；消极自我意识情绪的认知发展慢于积极自我意识情绪的认知。5 对情绪原因和情绪行为的认知在年级间差异显著，说明其在小学阶段有明显发展。6 对情绪表现者的情绪认知明显好于对情绪接受者的情绪认知。

The result indicated that HSC could obviously cut down the occurrence of rentricular vibration induced by chloroform in mice,lengthen the time of arrhythmia appearance,shorten the time of arrhythmia continuum and conversion induced by barium chloride in model rats,it also could shorten the time of ventriclar extrasystole and ventricular tachycardia in model rats induced by aconitine.

结果表明：黄松胶囊能明显降低氯仿所诱发小鼠室颤的发生，明显延长氯化钡模型大鼠心律失常出现时间，缩短模型大鼠心律失常持续和复律时间，尚能明显缩短乌头碱性大鼠心律失常的室性早搏及室性心动过速持续时间，与模型对照组比较均有统计学差异。

Furfur. At the same time, selected the culture supernatant. Used the whole-fat-cow"s-milk plate method, the Folin-phenol method and the QuantiCleave proteinase Assay Kit to test the proteinase activity of mycelial phase and yeast phase of M. furfur. The effect of temperature and pH on this assay was also evaluated.Result:①The whole-fat-cow"s-milk plate method: Seven days after inoculation, the proteinase activity of the mycelial phase supernatant (12.5 ± 1.2mm) is higher than that of the yeast phase (9.25±0.71mm)(p.05); With the passage of the inoculation time, the proteinase activity of the mycelial phase is rising; the temperature has no obvious effect on the proteinase

结果：①全脂牛奶平板法：培养第7天菌丝相上清液蛋白酶活性(12.5±1.2mm)高于酵母相(9.25±0.71mm)(p＜0.05)；菌丝相上清液蛋白酶活性随培养天数延长而增大；25℃和37℃两种温度对上清液蛋白酶活性无明显影响(P＞0.05)；②Folin-酚法：上清液蛋白酶活性随培养天数、菌落计数、菌丝诱导率增加而增大，培养液pH值呈下降趋势；同一天的菌丝相上清液蛋白酶活性明显高于酵母相(p＜0.05)；三种温度(25℃、37℃、49℃)及三种pH值(3.8、5.6、7.4)对上清液蛋白酶活性无明显影响；温度37℃、pH5.6时菌丝相上清液蛋白酶活性最高；③QuantiCleave蛋白酶试剂盒：菌丝相及酵母相上清液中均含有蛋白酶且

Can accelerate the bone marrow cell from G1 phase into S phase when we measure it's Cell cycle, it make S phase rate was increased .thus it promoted DNA synthesize and repaired. Mice irradiated at dose of 5.5 Gy gamma-irradiation showed timed-related decreases and restores in peripheral blood picture at day 1 through day 21, the counts of WBC of these group had taken drug has various degree to increase than irradiation control group in day 1. Grugs failed to protected the peripheral blood cell at lowest point, but seems promote their rescovery.9803 treated group was distinct. At 7days after mice were radiated in 3.5 Gy. we observed drug can Significantly hold back the decreased number of hematopoietic progenitors colony forming radiation induced. These findings indicated 9803 have a certain radioprotective activity against gammer irradiation in mice.

给药组的 CFU-S 数量较照射对照组明显增加，3.5Gy 照后第七天观察给药9803组对小鼠骨髓造血组细胞集落生成能力的影响，给药组的造血祖细胞集落的形成能力强于照射对照组，并且9803组明显优于523组。5.5Gy 照射引起的小鼠的外周血细胞数量的降低以 WBC 发生最早，照后24小时给药组的 WBC 数量均比照射对照组有不同程度的升高，以9803各给药组最为明显，药物未能使照射引起的外周血细胞最低值升高，而有降低的趋势，但给药对照射后期外周血象的总体恢复有较好的促进作用。7.5Gy 照射后第七天测定小鼠骨髓细胞周期的测定中发现9803具有促进骨髓细胞由 G1期进入 S 期的效应，致使 G0/G1期细胞显著减少， S 期细胞比率显著增加，这利于促进 DNA 合成修复，即促进骨髓细胞增殖，骨髓细胞 DNA 含量给药组显著高于照射对照组。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。