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The protection of AF-1 was observed with the measurement of transudatory rate of LDH ,the breaking rate of DNA and contents of LPO and SOD,Results:The intotoxincation of glutamate was decreased by AF-1 which reduced the transudatory rate of LDH,the breaking rate of DNA and the content of LPO,and increased the content of SOD in culture significantly.

通过测定乳酸脱氢酶漏出率、DNA断裂率、超氧化物歧化酶和脂质过氧化物,观察AF-1药物的保护作用。结果:高浓度的谷氨酸可增加培养神经细胞的LDH释放率和DNA断裂率,并诱导自由基产生。AF-1明显降低谷氨酸的神经细胞毒性,使LDH释放率及DNA断裂率明显降低,LPO含量明显下降,SOD含量明显升高。

The morphology of tumor cells was observed and the change of quantity was assayed by trypan blue exclusion.

结果在 ACV作用下,GRC- 1细胞的形态及数量无明显改变;GRC- 1/ TK细胞被明显杀伤,数量明显减少,当含 2 5 。0 %GRC- 1/ TK细胞的混合培养细胞即可观察到明显杀伤作用。

In the IR+SF group, the expression of iNOS was significantly lower than that in the IR+NS group. The levels of serum creatinine and blood uria nitrogen were significantly decrease.

结果 与C组比较,IRI+NS组iNOS表达明显增强(均P.01),血清Cr、BUN明显增加(P.01);与IRI+NS组比较,IRI+SFI组iNOS表达明显降低(均P.05),血清Cr、BUN明显降低。

Before the two-hour interaction of male-male individuals , we found there were obviously fewer and lighter stained ERp immunoreactive neurons (ER|3-IRs) in both main olfactory bulb system project regions and vomeronasal system project regions in the mandarin voles than in the reed voles, but no significant difference in numbers of AR immunoreactive neurons between the two species through the examining of immunoreactive neurons, moreover, there were significantly fewer AR-IRs than ERp-IRs in two species of vole before the two-hour interactions.

免疫组织化学检测,没有社会交往时棕色田鼠主嗅球系统投射区和犁鼻系统投射区ERβ免疫阳性细胞明显少于沼泽田鼠,且显色淡,AR免疫阳性细胞在两种鼠间差异不大,且都明显少于各自的ERβ-IRs.2h交往后,棕色田鼠主嗅球投射区和犁鼻系统投射区的ERβ-IRs细胞数明显少于交往前,AR-IRs细胞数明显多于交往前;沼泽田鼠交往前与交往后ERβ-IRs和AR-IRs细胞数均无显著差异,且显著多于交往后棕色田鼠ERβ-IRs细胞数,显著少于交往后棕色田鼠AR-IRs细胞数。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导,建立了MG63/R,其对顺铂的耐药指数为83.557±4.841,对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药;光镜观察可见MG63/R细胞排列不规则,形态呈三角形、多角形及多核现象;透射电镜显示MG63/R细胞表面突起增加,粗面内质网丰富;细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低;MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加,P53表达则明显降低。

The latent perios of N2 and P300 significantly prologed and the amplitude of P300markedly lowered occured in the following comparisons:comparing VP with PD and control group;comparing demented with nondemented VP group;comparing demented VP and demented PD group.

VP组的N2、P300潜伏期较PD组、正常对照组均明显延长,P300波幅较两者均明显降低。3.VP组痴呆者较非痴呆者N2、P300潜伏期明显延长,P300波幅明显降低,4。

Result: Shengdi injection could depress the increasing of the amount of total white blood cells and neutrophils and inhibit the increasing of TNF-α, O-2, MPO caused by LPS, as well as relieve the pathologic change including Neutrophils infiltrating and mucous edema in tracheae after intravenous administration. While it did not show the effect on monocyte, and histological lesion of the lung tissue.

结果:生地注射液静脉给药可明显抑制LPS诱导的白细胞总数和中性粒细胞数目升高,对淋巴单核细胞数目无明显影响;明显抑制TNF-α,O-2,MPO水平升高;明显抑制LPS诱导的肺组织中性粒细胞浸润、气管黏膜水肿,对肺组织损伤无影响。

Results After 3 months of kidney radiation by 15 Gy of Cs137 γ-rays, the changes with kidney shrank, Bowman's capsules straitened or disappeared, glomerular swelled, some epithelia denaturalized or died were observed. Mitochondria tumefied and the cristae shortened, lessened and disordered. Urine β2-MP rose evidently. It was lowered significantly the renal 25-OH-D-1α-hydroxylase activity and its mRNA expression. The dry and ash weight, BMD of femur and lumbar were significantly reduced, volume and width of bone trabecula were distinctly decreased and the space increased in model group. The Pyd / Cr increased and ALP decreased as well.

结果 15Gy 137Cs-γ射线肾局部照射3个月后肾脏体积缩小,肾小球肿大,Bowman氏囊囊腔狭窄甚至消失,部分肾小管上皮细胞变性、坏死,线粒体肿胀,体积增大,线粒体嵴变短、减少、排列紊乱;尿β2-MP显著增加;肾1α羟化酶活性及1α羟化酶mRNA基因表达量明显下降;肾辐射组股骨干重、灰重、腰椎和股骨BMD明显降低;骨小梁体积和骨小梁宽度减少,骨小梁间距增加,MAR减小;腰椎压缩和股骨三点弯最大载荷明显降低,Pyd / Cr值明显增加,ALP显著降低。

The individual density was 4.77 inds. per gram dry soil. The results indicated that the number of soil nematode fluctuated significantly with season, the number of soil nematode in autumn is significantly more than that in summer and in spring. Shannon-Wiener index, Pielou index, Margalef index and Trophic diversity index were used in analyzing the diversity of nematode community, ranking Cc>Pm>Clc, while Simpson index ranking Pm>Clc>Cc. The value of f/b, MI index, PPI index, MMI index, MI2-5 index, SI index and EI index were used in analyzing the characteristics of the functional structure of the nematode communities, ranking Cc>Pm>Clc, while PPI/MI value ranking Clc>Pm>Cc. It showed that the Clc was disturbed significantly.

研究结果表明,土壤线虫数量存在明显的季节波动,秋季明显高于夏季和春季;土壤线虫群落多样性指数、均匀度指数、丰富度指数和营养类群指数大小依次为阔叶林、针叶林和混交林,而优势度指数却表现为:针叶林>混交林>阔叶林;土壤线虫群落营养类群f/b值、MI 指数、PPI 指数、MMI指数、MI2-5指数、SI指数和EI指数均表现为:阔叶林>针叶林>混交林,而PPI/MI指数则表现为:混交林>针叶林>阔叶林,表明混交林受干扰明显;土壤理化特征与线虫数量、属数、生态指数间也存在明显相关关系。

The results showed:①Anthocyanin content in mature fruits was 132170 U·g-1FW in bud mutation which was very significantly higher than that in'Ralls'(49140 U·g-1FW);②Activities of PAL and UFGT were almost the same between the two materials, but CH I and DFR activitieswere significantly high in bud mutation, which were related to the increase of anthocyanin levels in the mutant;③Bagging treatment inhibited anthocyanin synthesis of bud mutation. But anthocyanin content had significantly increased after removing the bags. Activities of CHI and UFGT increased rap idly and were much higher than those in the control after the bag removal. The increase was consistentwith the imp rovement of anthocyanin levels.

结果显示:①在果实发育期间,芽变果皮的花青苷含量明显高于'国光',尤其是成熟期芽变果皮花青苷含量为132170 U·g-1FW,而'国光'仅为49140 U·g-1FW;②在果实发育期间,两个品种间PAL 和UFGT的酶活性无明显差异,但芽变的CHI和DFR酶活性明显高于'国光',表明芽变花青苷合成能力的提高与CHI和DFR酶活性高有关;③套袋抑制芽变果皮花青苷的合成,但解袋后花青苷的含量极显著升高,解袋后4种酶的变化趋势差异较大, CHI和UFGT活性均迅速升高,明显高于对照,这与解袋后花青苷迅速合成相吻合。

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