时间生物学

Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

However, my recent interviews with children living in the group of islands that includes Tertia show that these children spend much more time talking about their biological parents than about other adults in the village.

但是，我最近对生活在包括Tertia岛在内的该群岛上的孩子所作的访谈表明，这些孩子更多时间谈论的是其生物学意义上的父母而不是村子里的其他成人。

Because this kind of diesel oil has relatively high biological toxity and the residue is relatively permanent, people show more and more attention on how to remediate the polluted environment.

由于石油类具有较高的生物学毒性、在环境中的残留时间较长、产量大，因此如何对受其污染的环境进行有效修复日益受到社会关注。

A lot of the time his explanations of the biology and genetics of guppies is expressed in what appears to be a private language that is difficult to decipher but his keen eye and the breeding knowledge he has acquired from maintaining a fish room with a couple of hundred tanks is an invaluable source for my continuing research.

大部分的时间，他解释孔雀鱼的生物学以及基因学是用他自己的语言，一时很难理解，但是从他维持数百缸的鱼室中，他获得了独到的眼光以及饲育的知识，对我持续的研究是无价的来源。

Four pairs of primes were synthesized to amplify Salmonella genus special genes such as hut gene(495 bp), hilA gene(490 bp), invA gene(284 bp) and hns gene(152 bp) of Salmonella, respectively. The PCR reaction condition was optimized.

近年来，分子生物学方法如聚合酶链式反应在沙门氏菌的检测上已得到较好应用[1-6]，大大缩短了检测时间，但各报道中所用的DNA提取方法、引物、PCR反应条件各异。

But it indicates biological effect to the old larvers were refusing eating, extending the time of larver and pupating, shorteningthe time of pupa, affecting emergence, and causeing doomed of pupa and so on..

分别为16.83±5.18μg／ml、25.2±5.12μg／ml，对于3龄以上的棉铃虫幼虫则表现出拒食、影响生长发育、延长化蛹时间、缩短蛹历期、导致畸形蛹、引起蛹死亡及降低羽化率、产卵率和卵孵化率等生物学作用。

Based on the physiology of bone remodeling, a mechanical-biological factor coupled computational model at Basic Muhicellular Units level was put forward, which can describe the osteoporotic process of cancellous bond with time course.

根据骨重建的生理过程，在基本多细胞单位层次建立了一个力学因素和生物学因素耦联的，带有时间历程的松质骨骨质疏松过程计算模型。

This is a review of recent studies on the relationship between fire and invasive plant species.

在植物外来种与火生态因子的作用研究中发现，火与外来种的关系随物种生物学特性、火作用的时间、频度、强度不同而不同，火有时会有效地抑制外来种的生长和入侵，有时会促进一些外来种的生长和入侵。

Abstract］ objective to discuss the clinical meaning of the treatment of tibial plateau fracture by mipo and operation method.methods 34 cases of tibial plateau fracture with schatzker typeⅰ～ⅴ were treated by mipo during march 2005 to june 2007,then followed-up all the cases.results using mipo to treat the tibial plateau fracture in reducing bleeding and operating time,all cases had no tresis vulnus infection and necrosis,no fracture disunion,no loosening and breaking of internal fixations,etc.the knees function regained well.conclusion treating tibial plateau fractures with mipo has little obstructe in environment of fracture healing through minimally invasive,has fortes of minimally invasive,firm of internal fixations,low rate of infection,high rate of fracture healing,can reduce the complications,fit principle of biologic fixation.

目的 讨论应用微创接骨板固定术在治疗胫骨平台骨折中的临床意义及手术方法。方法运用mipo技术治疗2005年3月～2007年6月34例schatzkerⅰ～ⅴ型胫骨平台骨折，并对其进行随访观察。结果运用mipo技术治疗胫骨平台骨折，手术时间短，术中出血量少，没有发生伤口感染坏死、骨折不愈合和内固定松动、断裂等并发症，术后患者膝关节功能恢复良好。结论 mipo在治疗胫骨平台骨折时以微创来提供尽可能少受干扰的骨折愈合环境，具有创伤小、固定确实、感染率低、骨折愈合率高等优点，减少了并发症的发生，符合生物学固定的原理。

In the second part of this thesis. The biological process of porons HA/bBMP composite repairing experimental defect in the dog femora and monkey mandibular were investigated by the qualitative and quantitative methods. The results showed that not only were the speed and time of new bone ingrowing into the bBMP/HA composite much quicker and earlier than that of implants of HA alone, but also the quantity of new bone ingrowth into bBMP/HA composite was much higher than that of HA alone. The bone ingrowth of bBMP/HA composite at higher rate within 3 weeks. In this study, bBMP/HA promoted more active new bone formation in bone marrow tissue than did HA alone. This reveals that BMP, brought into the bone marrow with HA as a carrier, accelerates osteogenesis or osteoinduction, providing sime bone marrow cells with more differentiative potential.

论文第二部分应用定性和定量的方法，较全面地研究了HA/bBMP复合人工骨在修复狗股骨和猴下颌骨实验性缺损中的生物学过程，研究发现，新骨组织长入HA/bBMP复合人工骨的时间和速度都较单纯HA早和快，而且新骨形成量也明显高于单纯HA，HA/bBMP在挂入后早期(3周)的成骨最为活跃，自体骨髓组织可为BMP提供敏感的靶细胞，增强BMP的骨诱导效果和HA/bBMP复合人工骨的骨修复能力。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。