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Results:The results show that the survival rate of mammary epithelial cells were decreasing gradually with the increasing of the time of TGF-β1 treatment, and the survival rates of 24 h and 48 h groups were significantly lower than that of control group (p.05); the LDH activity of 6 h、12 h、24 h and 48 h groups were significantly higher than that of control group (p.05); the DNA degradation arisesed in 12 h, 24 h, and 48 h groups' mammary epithelial cells; the activity of Caspase-3 reach to a peak at 24 h, which was significantly higher than that of control group (p.05); the acinus of epithelial cells appears to break off and collapse with the increasing of treatment time, but the leydig structure keeps integrate;...

结果表明:1、5、10 ng/mL的TGF-β1均能抑制乳腺上皮细胞的增殖,且随浓度增加抑制作用加大,呈现剂量依赖性,其中10 ng/mL TGF-β1组与对照组差异显著(P.05);10 ng/mL TGF-β1作用于乳腺上皮细胞24 h,随作用时间延长细胞凋亡率逐渐增加,6 h、12 h和24 h组与对照组相比差异极显著(P.01);LDH活力也不断升高,6 h、12 h和24 h组与对照组差异显著(P.05);DNA随 TGF-β1作用时间的延长发生不同程度降解;TGF-β1作用2 h~6 h细胞大量表达HSP70,6 h达到高峰,而后下降,2~12 h的表达量均显著高于对照组(P.05)。结论:TGF-β1可以抑制奶牛乳腺上皮细胞的增殖,具有浓度依赖性。10 ng/mLTGF-β1。。。

One hour later, all of the animals were given each an intraperitoneal injection of 40 mg/kg of pentobarbital sodium or 200 mg/kg of barbital sodium.

结果:氯霉素组和3个剂量的CAD组均可使戊巴比妥钠睡眠时间延长,分别延至溶媒对照组的380%、284%、353%和366%(P.01);100mg/kg CAD组还可显著延长巴比妥钠催眠潜伏期(P.01),并缩短其睡眠时间(P.01)。

In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明,嗜热链球菌在营养丰富的培养基中的生长延滞期缩短,到达对数生长顶点的时间变短,稳定期的时间延长,当经过二次培养后,嗜热链球菌几乎不经过延滞期直接到达对数期,平稳期持续的时间较短,很快到达衰亡期;嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系,菌数多,其代谢旺盛,菌数降低其代谢速度减慢;嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化,并可能存在着细胞凋亡,与菌体增殖和产酸规律密切相关,随着培养时间延长,培养基中营养物质变得贫乏,菌体形态变化和细胞凋亡的情况愈加明显。

RESULTS: The experimental eyes were changed from hypermetropia at pre-experiment to high myopia quickly during experiment period, and the diopter degrees were deeper and eyeball axis were longer accompanying with the period elongation of form deprivation.

结果:实验组由实验前远视眼快速演变为高度近视眼,并随遮盖时间延长,近视度数加深,眼轴延长;两组眼球后壁均有VIPmRNA阳性表达;随出生时间延长两组VIPmRNA表达均逐渐增强。

The pugging process experiment of silica brick pug was made in Φ1 600×450 wet pan mill to study the change rule of main component mixing homogeneity and molding performance of the pug. The results showed that the pervasion and mixing concurred when diversified materials acceded to the wet pan mill, prolonging time in dry-mixed and wet-mixed periods had no action for increasing mixing homogeneity with pugging time prolonging, the molding performance was improved and the porosity of silica brick adobe had downtrend, but prolonging time after pugging 14 min the effect was not remarkable.

通过对Φ1 600×450湿碾机混练硅砖泥料过程中泥料主要成分的混合均匀度、泥料成型性能的变化规律研究,发现在各种物料加入湿碾机时,同时存在着扩散混合作用,延长干混、湿混阶段的时间,对于提高混合均匀度实际上没有意义;随捏练时间延长,泥料成型性能改善,砖坯气孔率呈下降趋势,但捏练14 min以后继续延长捏练时间,采用正常压制制度时,砖坯气孔率降低值已经很小。

Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement(more than 4 weeks),without significant differences between both groups.

结果 随着硬膜外腔置管时间延长,麻醉阻滞范围变窄,麻醉阻滞时间也明显缩短,两组之间没有明显区别;硬膜外腔造影显示:造影剂在硬膜外腔扩散良好,无明显受阻;组织学检查显示:置管时间长(4周以上)的动物,硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚,而且随置管时间延长,变化加显著,但两组之间没有明显区别。

Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement(more than 4 weeks),without significant differences between both groups.Conclusion Epidural corticosteroids can not prevent epidural adhesion from occurrance induced with long-term epidural catheter placement.

结果 随着硬膜外腔置管时间延长,麻醉阻滞范围变窄,麻醉阻滞时间也明显缩短,两组之间没有明显区别;硬膜外腔造影显示:造影剂在硬膜外腔扩散良好,无明显受阻;组织学检查显示:置管时间长(4周以上)的动物,硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚,而且随置管时间延长,这"种变化更"加显著,但两组之间没有明显区别。

Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement(more than 4 weeks),without significant differences between both groups.Conclusion Epidural corticosteroids can not prevent epidural adhesion from occurrance induced with long-term epidural catheter placement.

结果 随着硬膜外腔置管时间延长,麻醉阻滞范围变窄,麻醉阻滞时间也明显缩短,两组之间没有明显区别;硬膜外腔造影显示:造影剂在硬膜外腔扩散良好,无明显受阻;组织学检查显示:置管时间长(4周以上)的动物,硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚,而且随置管时间延长,这&种变化更&加显著,但两组之间没有明显区别。

Results: The renal perfusion was increased in diabetes group after the injection of alloxan. The PSI, AUC and ascending slope rate of the curve of the time-intensity curve were increased in 1w and 2w with decreased of HPT; the pathological examination exhibited glomeruli hypertrophy and no thickening of basal lamina. The PSI and AUC in 4w and 6w were more than that of control group; the PIT and ascending slope rate of the curve got closed to normal in 4w and 6w; the intercapillary cells increased. The speed of ascending slope rate of the curve in 8w, 10w and 12w was slower, shown as PIT and HPT delayed; the pathological examination showned increasing of mesangial cell, hyperplasia of mesangial matrix and thickening of basal lamina, followed with obliteration of partial micrangium, glomerular sclerosis and reduce of PSI in 12w.

结果:糖尿病组兔注射四氧嘧啶后肾皮质血流灌注增大,1周、2周表现为时间-强度曲线参数中峰值强度升高、曲线下面积增大、达峰时间缩短、曲线上升斜率升高,此时病理可见肾小球肥大,系膜基质正常,基底膜无增厚;4周、6周,峰值强度、曲线下面积高于对照组,达峰时间、曲线上升斜率接近正常,系膜细胞增多。8周、10周、12周,达峰时间延长、曲线上升斜率减慢、峰值强度减半时间延长,病理出现肾小球系膜细胞明显增多,系膜基质增生,基底膜增厚,至12周病理出现部分毛细血管管腔闭塞,肾小球节段性硬化,峰值强度测值减低。

The results showed that different topping frequency (at intervals of 15 d, 30 d, 60 d, 90 d) had no evident influence on total wine loss, volatile acid content, T-SO2,F-SO2, wine colour, and wine pH values. Under constant temperature and humidity conditions, total wine loss was closely correlated with the using degree of Chinese barrel and the storage time in Chinese barrel. Volatile acid content slightly rose along with wine storage time. The content of SO2 dropped rapidly during 15 d after wine filling, but dropped tardily ever since. Wine colority rose remarkably during 15 d after wine filling, but climbed tardily ever since. Wine colority change was unstable, it changed wavily and depressed on the whole. In conclusion, it was recommended that the best topping interval was 90 d.

结果表明,橡木桶15 d、30 d、60 d、90 d添桶间隔对红葡萄酒的酒液损失总量、挥发酸含量、T-SO2、F-SO2、颜色、pH值无显著影响;在恒温恒湿的条件下,红葡萄酒的酒液损失总量与橡木桶的新旧程度、桶贮时间密切相关;随桶贮时间延长,葡萄酒中挥发酸略有上升;葡萄酒入桶后15 d内SO2含量急剧下降,以后则缓慢下降;葡萄酒入桶后15 d内色度显著上升,但随后变化平缓,总的趋势是随桶贮时间延长色度缓慢上升;葡萄酒的色调变化不稳定,呈波浪式变化,总的趋势是降低;建议中国橡木桶桶贮红葡萄酒的添桶间隔为90 d。

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