时基

At the same time, the molecular n-n* absorption of nanoparticles were also blue-shifted, accompanied by a hardly changing n-π~* absorption as a result of the reduced overlap of the pyrazoline ringπorbital and a decrease of intermolecular interactions.

增大微粒的尺寸时从S_1态到CT念的荧光发射强度再分布表明，S_1念和CT态互相竞争并同时受纳米超微粒尺寸的影响。2、观察到了球形的1，3-二苯基-5-芘基-2-吡唑啉超微粒由于高度的单分散性而自组织排列成有序的二维阵列。

When BMSCs proliferated to enough cell number (7×106). 500 000 cells were centrifuged to a pellet and cultured in chondrogenic medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×106个细胞时，将每500 000个细胞离心制成高密度细胞微球，在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、 BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

When BMSCs proliferated to enough cell number (7×10^6). 500 000 cells were centrifuged to a pellet and cultured in chondrogenie medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×10^6个细胞时，将每500000个细胞离心制成高密度细胞微球，在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

The structure characters of this kind of molecules were discussed. The results show that the phenyl group located in the 4- or 5- position of pyrryl ring are not coplanar with the pyrryl ring.

对这类吡咯环上4或5-位有芳环取代基时化合物的晶体结构特征进行了扼要讨论，晶体衍射实验结果表明，4， 5-位上的芳环与吡咯环本身处于非共平面结构。

Radek Sikorski, the foreign minister, who broke the news to the prime minister, Donald Tusk, this morning said that the head of government wept on hearing it.

西科尔斯基向总理唐纳德？图斯克报告了这一消息，他今晨说，总理听到消息时失声痛哭。

In detaching the science of law from theology and religion , he prepared the ground for the secular, rationalistic version of modern natural law.

在将法学与神学和宗教分离时，他为现代自然法世俗的、理性主义的观点奠定了基

In the beginning, at the situation of high blood volume, if the blood glucose sufficient, the erythrocytic function of making ATP by glucose is normal, the free radical and the anti-free radical of the rat basicly keep balanced, erythrocytic deforming ability is not influenced greatly; while the erythrocytic function of making ATP degenerates in sport iron deficient erythrocyte formation and hemolysis, especially the former; and the vitalities of anti-free radical reduce greatly in blood, liver and Erythrocyte, the reducement of liver SOD vitality in the former and erythrocyte SOD vitality in the latter are more obvious, and the total blood SOD amounts reduce identically; The erythrocytic deforming ability reduces greatly too, but there is no obvious difference between the two groups, but the tendency of the inner-mechanism may be different needing to researching deeply later.

而长期递增的大负荷运动造成缺铁性红细胞生成或溶血发生时，红细胞利用葡萄糖制造ATP的功能下降，且前者下降更为明显；抗氧自由基活性均显著降低，HA组肝脏SOD活力和IDA组红细胞SOD活力降低更为明显，而二者血液总SOD降低一致；红细胞变形能力均显著下降，但内在机制的倾向性可能有所不同，建议进一步深入研究机理。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Results showed that the new type rhodanine ramifications were good performance fluorescence reagents to determine the trace matter by fluorescence analysis.

同时，通过对上述试剂在测定铁、钴、铜、锡和锑时的条件的变化的研究，对这类试剂的分子结构和荧光性能的内在关系作了简要的探讨，讨论了供、吸电子基团对胂酸基偶氮若丹宁类衍生物荧光分析性能的影响。

Under examination by Sabella, Schwimmer admitted he wrote an e-mail saying NYSE Chief Executive John Thain could "get cold feet" about the transaction.

在萨布拉质询时，史基莫承认，他曾在一封电子邮件中写到NYSE的首席执行长约翰。泰可能会在交易中＂临阵退缩＂。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。