时基

Structures, there was an important issues in the jointless design of R.C. superlong structure.

对平面尺寸不大的结构来说，认为基底各点输入的地震激励是一致的尚且可以；但若结构的平面尺寸很大，或者结构是像桥梁那样的长跨、多跨结构，则此时再对结构基低各点和各支点输入同一地震时程进行分析就不尽合理了。

Progress to date has been slow, on the surface at least, with Sunderland having found themselves linked with a series of targets but yet to make the breakthrough. Preston North End striker David Nugent and Bolton Wanderers goalkeeper Jussi Jaaskelainen have headed the list thrown up by the rumour mill, although Keane has given nothing away.

一切进展都很慢，至少表面上来看是这样的，当桑德兰发现他们的几个目标时，他们还没取得实质性的突破，普雷斯顿前锋大卫努金特和博尔顿守门员雅斯科莱宁已经进入转会传言中，尽管基恩一个球员都没弄到手。

Alexander Paulin was born in Kiel in 1967 and photographed since his 14 birthday.

亚历山大保1 967年出生在基尔，从14岁时开始摄影。摄

Alexander Paulin was born in Kiel in 1967 and photographed since his 14 birthday.

亚历山大保1 967年出身在基尔，从14岁时开端。

Kieran Lee was initially a midfielder when he first arrived at United, but his versatility allows him to play equally well in almost any position.

基兰李刚到曼联时最初打的是中场，但他全面的技术特点使得他在任何一个位置都表现出色。

"The answer to when we will start to come out of that trough lies in when the economy comes out of that trough," Kolinski Morris said.

&回答我们将什么时候开始走出谷底以及经济什么时候开始复苏这个问题时，&科林斯基莫里斯说。

Chinensis and Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible NOS activity also decreased significantly with the time elapsed in WSSV infected shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization test showed that WSSV of the two penaeid species couldn't be detected until iNOS started decreasing.

在此基础上，我们建立了对虾血细胞中诱导型一氧化氮合成酶的鉴定方法—硝基四氮唑蓝还原法和血细胞形态法以及酶活力的测定方法—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件，结果显示：对虾血细胞在4℃下与L-精氨酸和LPS孵育8h以及当L-精氨酸浓度为2.5mmol l〓LPS浓度为100μg ml〓，Ca〓浓度为2.5mmol l〓时，iNOS活力的测定结果最佳。

The result fights allergic experiment to show, dispel the wind stops land of can different level restrains urticant Ding allergy of akin and passive skin mixes the bandicoot that antiserum causes 2, the sex of small rat late hair of chloric benzene be caused by exceeds 2 nitro- of 4- quick the thymus index that reaction; still can increase small rat when dosage greatly and lienal index; stop urticant experiment shows, dispel the wind stops urticant Ding can reduce the time of fit of small rat itching with dextrose lead anhydride and itching duration apparently, can increase Guinea pig to be able to bear or endure those who suffer phosphoric acid histamine send urticant threshold.

结果抗过敏试验显示，祛风止痒酊能不同程度地抑制抗血清诱发的大鼠同种被动皮肤过敏和2，4-二硝基氯苯所致的小鼠迟发性超敏反应；大剂量时还可增加小鼠的胸腺指数及脾指数；止痒试验显示，祛风止痒酊能明显降低右旋糖酐诱导的小鼠搔痒发作次数及搔痒持续时间，并能增加豚鼠耐受磷酸组胺的致痒阈。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

BiographySimon Baker was first recognized in 1992, when he received Australias prestigious Logie award for Most Popular New Talent.

演员小传：西蒙巴克于1992年被评为澳大利亚洛基奖&最受欢迎新秀&时初露头角，刚刚举家移居洛杉矶后，巴克就参演了奥斯卡得奖影片《洛城机密》（1997）。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。