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无足细胞

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The Vg-negative reaction of the triploid female rainbow trout could not provide full evidence for the impossibility of liver to synthesize Vg. That Vg was not synthesized resulted from insufficient estradiol due to the retardation of ovaries development, the un-differentiation of follicular cells and the lost interaction of germ cell and somatic cells. It could be concluded from yolk genesis that the shortage of female triploid rainbow trout's Vg resulted from the abortive ooecium, but did not result in sterility.

由于三倍体雌性虹鳟卵巢发育受阻,无滤泡细胞分化,生殖细胞与体细胞的互作缺失,不能分泌足量的17β-E2以诱导Vg的合成,因而肝脏的Vg阴性并不能说明肝脏不具备合成Vg的能力,从卵黄发生的角度分析,卵黄蛋白原的缺乏不是导致三倍体虹鳟雌性不育的原因,而是其卵泡败育的结果。

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果: hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙,对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比,统计学上无显著差异(P>0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂,再共同作用48 h,CEM活细胞均数为2.318×108 cells/L,与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比,对细胞抑制明显增强(P<0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h,细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P<0.01)。

Hill-reservoir has a short retention time; its phytoplankton community was characterized by low abundance and no obviously dominant species. The zooplankton was predominated numerically by rotifer and nauplii.

山地型水库具有较明显的河流特征,浮游植物的种类数和细胞密度均较低,有较多的河流种,无明显优势种;浮游动物的种类和数量很低,以轮虫和桡足类幼体为主。

Results: in contrast with model group, SSM can (1) increase the survival rate of ARF in rats, cut down Scr and BUN at 24hr, improve renal function, slightly surpass Vp in its therapeutic effect;(2) mitigate the extent of renal pathologicchanges, decrease the numbers of renal tubule necroses and casts;(3) protect renal ultrastructure such as microvilli, mitochondria, endoplasmic reticulum of TEC, podocytic process of glomerulus epithelial cell,endothelial cell etc, better than Vp;(4) enhance SOD activity, lower MDA contents in renal cortex;(5) enhance NO contents in serum, reduce ET levels in plasma;(6) evidently cut down TNF- a contents in serum, superior to Vp;(7) antagonize calcium overload in TEC;(8) alleviate renal pathological changes in ARF metaphase (at 3d), advance regeneration and recovery of TEC, restore renal structure in ARF convalescence (at 5d), and better than Vp;(9) increase the expression of TEC PCNA and prepro EGFmRNA in ARF metaphase.

结果:与模型组相比,SSM能(1)提高ARF大鼠的存活率,降低24hr时Scr及BUN,改善肾功能,其疗效略优于维拉帕米;(2)减轻ARF肾脏病变程度,减少24hr肾小管坏死数及管腔内管型数;(3)对肾脏的超微结构如肾小管上皮细胞微绒毛、线粒体、内质网、肾小球上皮细胞足突、内皮细胞等形态结构均有一定的保护作用,其疗效略优于维拉帕米;(4)提高肾皮质SOD活性,降低MDA含量;(5)提高血清NO水平,同时降低血浆ET含量;(6)显著降低血清TNF-α的水平,疗效明显优于维拉帕米;(7)拮抗小管上皮细胞[Ca~(2+)]_i的升高;(8)使ARF中期(第3d时)肾组织病变较同期其它两组明显减轻,并使肾小管细胞再生修复提前,至ARF恢复期(第5d时)肾组织结构恢复重建良好;作用明显优于维拉帕米;(9)促进ARF中期肾小管上皮细胞PCNA的表达及EGF前体mRNA的合成,从而增加EGF的合成释放,而维拉帕米无此作用。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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