无蛋白质的

In addition, the addition of Si ni tang to culture of D.radiodurans could induce cell growth and protein expression. In this study, 2-D electrophoresis and Matrix Assisted Laser Desorption Ionization Mass Spectrometry were used to analyze the proteomic differences between adjunction of Mn and Si ni tang in normal cells.

实验方法是利用二维蛋白质电泳(2-D Electrophoresis)和基质辅助雷射脱附游离质谱仪(Matrix Assisted Laser Desorption Ionization Mass Spectrometry；MALDI/MS)来分析耐辐射奇异球菌在有或无锰离子与四逆汤的培养下，其菌体表现出的蛋白质种类与数量的差异。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

Result The background noise of bidirectional electrophoresis map of protein extracted by TCAJA method was greater, its clarity was poor, its horizontal striations were more and there were tinny spots with irregular shape appeared on it. The variegated spots had difference from the normal protein spots, they formed high coverage and shading on the other protein spots and affected the accurate expression of map information. The background noise of bidirectional electrophoresis map of protein extracted by Trizol method was little, its horizontal striations and non-protein impurities were less, there was no shading phenomenon strong spots and mere protein spots, so it provided abundant information for quantitative and qualitative analyses on the proteome from buffalo grass seeds, Compared with TCA/A precipitation.

结果］TCA/A法提取的蛋白质双向电泳图谱背景噪音大，清晰度差，横向条纹较多，图谱上呈现多个形状不规则的斑点，杂色与正常蛋白质点有区别，对其他蛋白质点构成高度及盖和遮进，影响图谱信息的准确表达；Trizol法所提取的蛋白质样品，其双向电泳图谱背景嗓音小，横向条纹少，非蛋白类杂质少，无斑点间的这进现象，蛋白质点数量增多，为野牛草种于蛋白质组定量、定性分析提供了丰富的信息。

The main product for selling is 'wondermilk', the milk fountainhead is use 100% nature, high quality, fresh milk, without any additives, bst, antibiotic. our milk adopt pasteurized which can keep all kinds nutrition of fresh milk. the lactoprotein content is more than 3.5%/ml. our milk gets the broad recognized and highly valuable in the largeness foreigners with purity taste and cleanlily.

所生产销售的主要产品"万得妙纯牛奶"，其乳源全部选用100%纯天然的高品质生鲜乳，无添加物，无生长激素，无抗生素，经过八氏灭菌处理，保留了新鲜乳中各种特有的营养，其中乳蛋白质高于3.5%/100ml，以其口味纯正，清爽，在广大涉外等客户群中得到了广泛的认可，并得到高度评价。

Experiment 2: To choose the appropriate dosage of tannins for the diets in sheep, three High Mountain Fine-wool wethers fitted with permanent rumen fistula were used and the 3 ×3 Latin square design were adopted to study the effects of tannins in Sainfoin on protein protection of the diets, measured items included the rumen metabolic parameters, the appatent digestion of all nutrients and the appatent retention of N in sheep.

试验二：选用3 只安装有永久性瘤胃瘘管的高山细毛羊羯羊，采用3×3 无重复拉丁方试验设计，研究红豆草中单宁对饲粮蛋白质保护的效果，测定项目有绵羊瘤胃代谢参数和饲粮各种养分的消化率与氮存留率，以期初步筛选出绵羊饲粮中适宜的单宁用量，为提高蛋白质饲料的有效利用提供依据。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

Based on the analysis of ORF , the two cDNAs encode the respective proteins of 541 and 555 amino acids. The two proteins are 77% identical in amino acid sequences, and they have not significant homologies with all known proteins.

根据读码框架分析，这两个cDNA分别编码541和555个氨基酸的蛋白质，两个蛋白质之间氨基酸序列一致率为77％，和已知蛋白无显著同源性。

MS detection showed the six protein spots were inorganic pyrophosphatase, peptidyl-prolyl cis-trans isomerase A, elongation factor Tu, unnamed protein, dioxygenase and moxR protein. Conclusion Protein expression differences were discovered between multi-drug-resistant Mycobacterium tuberculosis isolate and drug-sensitive isolate.

质谱分析发现，6个蛋白质点分别代表的蛋白质为无机焦磷酸酶、多肽脯氨酰基顺反同分异构酶A、延伸因子Tu、420个氨基酸的未知功能蛋白质、双加氧酶和转录调节蛋白。

Conversely,the content of crude fiber,dry matter and nitrogen-free extract were high in setting stage although the content trends aneurolepidium dasystachys has a little different.

其中赖草的干物质、蛋白质、粗脂肪、无氮浸出物和粗灰分含量显著高于川草ⅰ号、川草ⅱ号、垂穗鹅冠草和硬杆仲冰草等4种草。5种草不同生育期营养成分的分析结果表明，各草种分蘖期和孕穗期的粗水分、粗灰分、蛋白质、粗脂肪以及钙、磷含量明显高于结实期，而粗纤维、干物质和除赖草外的无氮浸出物含量则相反。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐