无菌法

Pharmacopeial articles are to be tested by the Membrane Filtration method under Test for Sterility of the Product to be Examined where the nature of the product permits.

只要其性质许可，这些药品将使用供试产品无菌检查法项下的膜过滤法来检测。

ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

选择健康杂种犬36只，无菌抽取犬骨髓液10 mL，经Percoll液密度梯度离心后贴壁法体外培养骨髓间充质干细胞，传2代后行5-氮胞苷诱导，5-溴脱氧尿苷标记备用。

METHODS: Triceps brachii was sterilely collected from 3 rats, and muscle-derived stem cells were isolated in vitro, and purified by adherence method.

取3只大鼠，无菌条件下切取肱三头肌，体外分离培养肌源性干细胞，贴壁法纯化扩增。

ICR fetus mice were sterilely obtained to culture mouse embryonic fibroblasts by tissue pancreatin digestion method.

无菌条件下取出ICR胎鼠，组织块胰酶消化法分离培养小鼠胚胎成纤维细胞，接种在0.1%明胶包被的6孔板中即为饲养层。

METHODS: Fasting venous blood of participants in two groups was harvested sterilely at 6 months and 1 year after test group restoration, serum nickel and chromium content were determined using graphite furnace atomic absorption spectrometry.

无菌抽取对照组健康者及实验组患者修复后0.5年，1年空腹静脉血，采用石墨炉原子吸收光谱法测定血清中镍、铬元素的含量。

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。

Membrane filtration method is a validatory method in the sterility test of little capacity injection, as well as di...

结论在小容量注射剂无菌检查中薄膜过滤法可以代替直接接种法，方法简便、可靠。

Methods Forty-four Wistar rats were randomly divided into sham operation group (SO group, n=12), operation group (n=16) and carbachol treated group (carbachol group, n=16, carbachol 50 μg/kg). Animal model of abdominal adhesion was established by rubbing the procussus vermiformis of cecum with dry sterile gauze, and by clamping and scuffing abdominal wall.

44只雄性Wistar大鼠按完全随机法分为假手术组(n=12)、手术对照组(n=16)及卡巴胆碱组(n=16，卡巴胆碱50 μg/kg)，后2组采用开腹后无菌干纱布摩擦大鼠盲肠蚓突部，钳夹和刮伤腹壁法制作大鼠腹腔粘连动物模型。

Methods: MSCs were isolated from bilateral thighbones and tibias of Wistar rats, purified by adhesive-screening method, expanded in vitro culture and labeled with DAPI.

无菌条件下取出大鼠双侧股骨及胫骨，冲洗骨髓腔获得细胞，贴壁筛选法纯化MSCs并进行体外培养、扩增。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。