无菌法

In vitro, we cultured human and rabbit retinal Müller cells by way of mixed enzyme digestion and tissue adherence, using human retina of an abortus of 6 months and healthy rabbit.

取孕6个月引产胎儿，死后2h内无菌条件下摘取眼球，剥取视网膜组织，混合酶消化法培养人视网膜Müller细胞；取健康新西兰大白兔，死后以最快的速度无菌条件下摘取眼球，组织块贴壁法培养兔视网膜Müller细胞；利用含胎牛血清的高糖DMEM作为培养液，不添加生长因子。

Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.

石蒜属植物快速繁殖技术研究中，在无菌无性系建立阶段：鳞茎以0.1％HgCl_2消毒10-12分钟效果最好，而叶、根尖、鳞芽等较干净部位选用0.1％HgCl_2消毒7分钟；用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养，以带基盘鳞片诱导分化效果最理想，鳞芽易培养出芽，但数量有限，而叶片、根尖与不带基盘鳞片均未诱导分化；带基盘鳞片为石蒜属植物快速繁殖最佳外植体，以鳞茎中部3-15层芽诱导率高，较外层稍次之，内部鳞片诱导率最低；选用八等分法、六等分法、四等分法切割鳞茎，六等分法综合效果最好；每外植体带三鳞片培养最为适宜；三种石蒜属植物各自适合的培养基成分不同，换锦花在MS+BA5mg／l+NAA0.1mg／l中培养效果最好，夏水仙为MS+BA5mg／l+NAA0.5mg／l，而白花长筒石蒜在各培养基组合中培养效果均不理想，其适合的培养条件有待于进一步研究。

Methods:Bacteriostatic ring after both using together is assayed at asepsis condition by microbion s well diffusion means with using Sarcina lutea as bacterial suspension and ampicinin and erythromycin as model drug.

采用微生物井式扩散法，在无菌条件下，用藤黄八叠球菌作菌悬液，氨苄西林、红霉素标准品为模型药物，测定两者合用后的抑菌圈。

Methods: The blood samples were collected from Gram negative bacterial infection and deep mycosis patients and healthy volunteers.

在无菌条件下，留取 20例正常人、10例严重腹腔革兰阴性菌感染及 5例深部真菌感染病人的血浆，经前处理后，用MB 80系统动态浊度定量法， 1h完成检测。

Digital planimetry method ; ImageJ-ij133-jdk15; Transparency tracing method ; Diabetic cutaneous ulcer ; Wound measurement ; Multiple l inear regression ; Intraclass correlation coefficients

数码相机拍摄法； Image J 医学图像分析软件；无菌薄膜勾边法；糖尿病皮肤溃疡；创伤面积测量；多元线性回归；组内相关系数

Employing capillary-pipette washing method,the unialgal strain and axenic strain of Microcystis aeruginosa from Songhua Lake were isolated and purified,and effects of temperature,illuminance,nitrogen,phosphorus,and iron on their growth were investigated.

用毛细滴管洗净法从松花湖分离纯化出铜绿微囊藻的单藻株和无菌株，研究了温度、照度、N、P、Fe各种因素对单藻株和无菌株生长的影响。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

METHODS: Bilateral sciatic nerve was sterilely dissociated from rats. Schwann cells were prepared and purified by low-concentration enzyme fast digestion and differential adherence.

无菌剥离大鼠双侧坐骨神经，应用低浓度酶快速消化法、差速贴壁法分离培养纯化许旺细胞。

9 healthy adult Holstein cows were selected as experimental cows and they were divided into 3 groups according to different milk lactations. Individual tracking method was used to observe and record the amount of Diptera insects. The blood was took on the tail vein under the aseptic condition and then the serum was extracted. The level of ACTH, COR, PRL, GH and NE in the serum were detected by the method of radioactive immunoassay method and fluorescence spectrophotometry.

方法］选择9头成年健康的荷斯坦奶牛作为试验牛，按不同泌乳阶段分3组，采用个体跟踪法观察并记录侵袭试验牛双翅目昆虫的数目，自尾静脉无菌采血，提取血清，采用放射性免疫法和荧光分光光度法测定血清中AGTH、COR、PRL、GH和NE的水平。

The recombinant plasmid pBI121-VP6 was transformed into Agrobacterium tumefaciens strainLBA4404 by freeze-thawing method and the introduced into the sterile tube culture shoot of tobacco NC89 and baoding alfalfa by leaf disc infection method.

以冻融法将pBI121-VP6重组质粒导入根癌衣杆菌LBA4404，用叶盘法转化于NC89烟草和保定紫花苜蓿的无菌试管苗。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。