无菌操作

Familiar with aseptic technique and analysis instrument operation; strong hand skills.

熟悉无菌操作、分析仪器的操作、动手能力强

Aseptically remove a sufficient quantity of solids from the appropriate amount of containers (see Table 2), mix to obtain a composite, equivalent to about 6 g of solids, and transfer to a sterile 500-mL conical flask.

以无菌操作从适当数量的容器中（见表2）取出足够数量的固体，混匀以获得等同于6g固体的混合物，转移至一个无菌的500mL锥形烧瓶。

Storage of drug products after release;(9) Control and laboratory operations;(10) Aseptic processing, which includes as appropriate: I Floors, walls, and ceilings of smooth, hard surfaces that are easily cleanable; II Temperature and humidity controls; III An air supply filtered through high-efficiency particulate air filters under positive pressure, regardless of whether flow is laminar or nonlaminar; IV A system for monitoring environmental conditions; V A system for cleaning and disinfecting the room and equipment to produce aseptic conditions; VI A system for maintaining any equipment used to control the aseptic conditions.

无菌操作及有关要求： I 地板，墙壁和天花板平滑，坚硬，表面易清洁； II 温度与湿度控制； III 空气经高效过滤器，在正压下过滤，层流或非层流均可； IV 环境检测系统； V 创造无菌环境，房间和设备的清洁，消毒系统； VI 控制无菌环境的设备维修系统。

Counting method of microorganisms in environment;②preparation of smears for observing forms and structures of bacteria、Actinomyces、yeasts and molds, methods and technologies of staining and oil immersion objective;③cell counting techniques and microscope examination techniques;④preparation of microbic media and sterilization techniques;⑤aseptic operations and microbic inoculation methods ;⑥technics of microbic separation、purification and cultivation;⑦physiological and biochemical reactions of microorganisms and the microbial examination techniques of food sanitation to strengthen students the operation techniques.

课程内容：本课程实验内容包括：①环境中微生物检测计数；②细菌、放线菌、酵母及霉菌细胞形态、结构的制片，染色和显微镜观测，油浸物镜的使用技术；③细胞计数技术和显微测量技术；④微生物培养基制备、压力蒸气灭菌技术；⑤无菌操作技术、移种技术；⑥微生物分离、纯化和培养技术；⑦微生物的生理、生化反应及食品卫生微生物检测技术等旨在加强学生微生物学的操作技术。

Of various patches, synthesized nonabsorbable patch and acellular patch display low infection, little complication and good biocompatibility.

补片植入操作时尽量将生物补片置于无张力状态，严格无菌操作，严密止血即可降低感染和侵蚀的发生率。

Methods Ninety elective patients undergoing general anesthesia for hysterectomy were randomly assigned to two equal groups. GroupⅡreceived endotracheal intubation protected by sterilized transparent sleeve while groupⅠcorrespondingly adopted unprotective classical endotracheal intubation. Endotracheal swab sampling and bacterial counting were performed on the principle of aseptic processing before endotracheal intubation and extubation, respectively.

90例在全身麻醉下行择期全子宫切除术的患者随机分为两组，每组45例，Ⅰ组采用传统的气管插管操作方法，Ⅱ组采用无菌透明隔离套保护的气管插管操作方法，在气管插管前和拔管前分别按照无菌操作原则实施气管内试子取样，进行菌落计数。

To master the local anatomy and operation main points well is the key of catheterizing successfully.

结论掌握好局部解剖位置和操作要领是置管成功的关键，严格无菌操作是预防导管感染和堵管的关键。

One stage has included filling of 3000 vials and stoppering on line; another stage included filling 3000 vials, transportation to the lyophilizer and then stoppering; a third stage included the filling of 3000 vials, loading in the lyophilizer, and exposure to a portion of the nitrogen flush and then stoppering.

第一部分是灌装3000瓶并且加塞，第二部分是灌装3000瓶，传递到冻干机中再全加塞，第三部分是灌装3000瓶，排放到冻干机内，暴露在氮气流下冻干机的灭菌和用于回填的无菌氮气系统分别进行验证，培养基灌装主要验证灌装、传递和摆放的无菌操作。

Has included filling of 3000 vials and stoppering on line; another stage included filling 3000 vials, transportation to the lyophilizer and then stoppering; a third stage included the filling of 3000 vials, loading in the lyophilizer, and exposure to a portion of the nitrogen flush and then stoppering.Since lyophilizer sterilization and sterilization of the nitrogen system used to backfill require separate validation, media fills should primarily validate the filling, transportation and loading aseptic operations.

第一部分是灌装3000瓶并且加塞，第二部分是灌装3000瓶，传递到冻干机中再全加塞，第三部分是灌装3000瓶，排放到冻干机内，暴露在氮气流下冻干机的灭菌和用于回填的无菌氮气系统分别进行验证，培养基灌装主要验证灌装、传递和摆放的无菌操作。

The course mainly introduces basic principles and methods of experimental techniques of cell engineering, which includesmedia preparation, sterilization and aseptic technique, virus-free seedling production, plant rapid propagation, immature embryo culture, pollen and anther culture, cell culture, protoplast culture, somatic hybridization, plant transformation, and so on. The contents of this course follow the from easy

该课程主要介绍细胞工程特别是植物细胞工程的基本实验技术及其原理与方法，包括：培养基配制、灭菌及无菌操作技术、无病毒苗培育、植物快速繁殖、幼胚培养、花药及花粉培养、细胞培养、原生质体培养、体细胞杂交及植物遗传转化等。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失