无菌技术

The results showed that all the strains of A. avenae subsp.citrulli tested produced 360bp specific fragments by the ISE and the direct PCR method, while others including 23 strains of Pseudomonas, Xanthomonas, Erwinia, Clavibacter, Burkholderia and Bacillus showed negative PCR result.

用直接PCR技术和免疫捕捉PCR技术检测西瓜果斑病菌，结果表明，所有果斑病菌都能产生360bp左右的特异性片段，而对照Pseudomonas、Xanthomonas、Erwinia、Clavibacter、Burkholderia及Bacillus的23菌株无特异性片段产生。

Based on the results of 1H-NMR, 13C-NMR, ESI-MS and UV spectra, the structures of three compounds with antitumor activity were identified as Prodigiosin、7-Hexadecenoic acid、7,10-Octadecadienoic acid, respectively; another compounds (no activity to above tumor cells)was identified as Myristic acid.

运用核磁共振（1H-NMR、13C-NMR）、质谱、紫外光谱等现代波谱技术分析，将其中3种具有抗肿瘤活性的化合物分别鉴定为灵菌红素、7-十六碳烯酸（7-Hexadecenoic acid）、7，10-十八碳二烯酸（7，10-Octadecadienoic acid）；鉴定另一种对上述肿瘤细胞无活性的化合物为肉豆蔻酸。

Air is clean spend and fall next bacterium are current air of product of province IVC system, isolator purifies Jiangsu to roughly two kinds of air current organize means, one kind sends a top to answer for the top: IVC remands wind filters through containing directly top of basket case case, horniness isolator remands on ben top wind; remands another kind to be the same as one side parallel: IVC remands wind contains filter film with a side face in basket box undertake inside aperture, soft qualitative isolator is sent by ben a side wind is answered on wind, means of two kinds of air current all is be not one-way and popular form.

目前实验动物相关国家标准对此类设备各项技术指标的检测并无说明，笔者对该类产品按江苏省地方标准[1]检测进行了初步的探索。1空气洁净度和落下菌目前江苏省IVC系统、隔离器产品空气净化大致有两种气流组织方式，一种为顶送顶回：IVC送回风直接通过含有过滤膜的笼箱盒盖，硬质隔离器在内室顶上送回风；另一种为同一面平行送回：IVC送回风在笼箱同一侧面含有滤膜孔内进行，软质隔离器由内室一侧送风对面回风，两种气流方式均为非单向流行式。

Methods: Differences of cellular and extracellular soluble protein expression between high cariogenic potential streptococcus mutans 593 clinical isolate and low cariogenic potential streptococcus mutans 18 clinical isolate were compared by SDS-PAGE.

采用SDS-PAGE技术，比较分离自高龋患者的变形链球菌593号菌株和无龋健康人的变形链球菌18号菌株的菌体和菌外可溶性蛋白表达的差异。

The characteristics and trends in material research／development at present are discussed,focusing on the new progress in material research done by the Institute of Metal Research CAS.What were studied include ultra-high strength maraging steel,nickel-free austenite stainless steel for medical applications.coronary artery bracket for drug's slow release,antibiotic stainless steel and metal hydride for the use of air conditioner.

摘 要：首先分析了材料研究与发展中呈现出的新特点和新趋势，着重介绍了中国科学院金属研究所相关课题组近年来在超高强度马氏体时效钢、新型医用无镍奥氏体不锈钢、缓释药物冠动脉支架、抗菌不锈钢、金属氢化物空调等新材料及新材料技术方面的研究新进展。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。