无菌

In order to clarify the genetic mechanism of the adhesion,an in vitro adhesion experiment was carried out for three variants of E.coli F4in 366 piglets of three pig breeds.The results showed that there existed significant differences(P.001)in the adhesion percentage among the three breeds.Most SB piglets were nonadhesive for all the three variants,whereas most LR piglets were adhesive.Within each breed except for LR,the proportions of the three F4 variants adhering to the brush borders differed significantly.

为了研究黏附的遗传机制，本实验利用大白、长白、松辽黑猪的小肠刷状缘细胞与F4ab、F4ac、F4ad进行离体黏附实验，结果发现3品种猪之间黏附情况存在显著差别(P《0.01)，松辽黑猪以非黏附型为主，而长白猪中黏附型比例较高，在同一品种猪内，3种菌株的黏附比例在松辽黑猪内和大白猪内有极显著差异，但在长白猪中无显著差异。

G at a low dose, sign of clinical infection was not observed and case of death was not found, mimicking the nonsymptomatic bacteremias model.

结论兔髂动脉球囊剥脱术后，给予或不给予高脂饮食，通过小剂量P.g静脉注射，可建立伴无症状菌血症的兔动脉粥样硬化模型。

The most common microbes causing infections in percutaneously inserted uncuffed catheters are, in order of prevalence, coagulase-negative staphylococci, Staphylococcus aureus, Candida species, and enteric gram-negative bacilli.

根据患病率的高低，引起经皮插入的无气囊导管感染的最常见的微生物排序如下：凝固酶阴性葡萄球菌、金黄色葡萄球菌、念珠菌属菌种和肠道革兰氏阴性细菌。

Lift again as phthisical incidence of a disease, n/med tuberculosis of the film inside bronchus also is added gradually much, because n/med tuberculosis of the film inside bronchus is inchoate the symptom is not typical, phlegmy bacterium examination often is negative, bosom X line, CT does not have diagnostic sex sign again, reason hard make a diagnosis and give treatment.

概要： 随着肺结核发病率再次升高，支气管内膜结核也逐渐增多，支气管内膜结核因早期症状不典型，痰菌检查常为阴性，胸部X线、CT又无特征性征象，故难以诊治。

Avermeetin has no contract poisoning toxicity to eggs and no effect on adult lifespan and mean number of eggs of Plutella xylostella .

阿维菌素对小菜蛾卵不具触杀毒力，对成虫寿命及产卵量无明显的影响作用。

The S. pullorum on the surface of shell eggs was completely killed and E. coli O157:H7 logarithm of counts decreased to less than 1.0 log10 by NEW containing 12 mg/L available chlorine for 3 min without viable cells in the washing water after treatment.

当中性电解水清洗消毒被人工污染的鸡蛋表面时，有效氯质量浓度为12 mg/L、处理3 min可将鸡蛋表面的鸡白痢沙门氏菌全部杀灭，大肠杆菌O157：H7菌落数对数降低到1.0 log10 以下，且处理废液中没有残存菌，无二次污染问题。

ResultsCyromazine had a great toxicity to firs t-instar larvae of housefly with LC 50 value of 43.896 mg/L, and showed less toxic actio n to third-instar larvae and adults of housefly, but reduced the pupate, ecl osion, female oviposition and egg hatch.

结果 灭蝇胺对家蝇 1日龄幼虫的 48h的LC5 0 值为 43 。896mg/L ，对 3日龄幼虫和成蝇的毒杀效果较差，但可引起幼虫化蛹率、羽化率以及雌成蝇产卵量和卵孵化率明显下降；阿维菌素对家蝇 1日龄、3日龄幼虫和成蝇表现出较强的急性毒杀作用，其 48hLC5 0 值分别为 0 。162 1mg/L、1.5546mg/L和 0 。2 10 3mg/L ，而对家蝇生长发育无不良影响。

The result expressed that under the concentration of 0.2mg/mL, the 6 compounds are all active to Sarcina lutea, ME-1, MT-6, MT-land MT-7 are active to Bacillus megaterium, to Escherichia coli, only MT-2 is active. The dimeric natural products are intensively existed in botany, most of them have the obvious biological activities. They have been concentrated on in recent years, and they are one of the compounds to which are enjoyed great popularity in natural product.

结果表明，在浓度为0.2mg/mL的条件下，六种化合物对藤黄八叠球菌均具有明显抑制作用，ME-1、MT-6、MT-1、MT-7对巨大芽孢杆菌有抑制作用，MT-2对大肠杆菌有抑制作用，对绿脓杆菌和枯草芽孢杆菌六种成分均无抑制作用；二聚体化合物在植物界广泛存在，多数二聚体天然产物具有显著的生物活性，引起人们的重视，是天然产物化学界研究的热门化合物之一。

The results of bioassay indicated: 1 The new compound (compound 6) and other three compounds (compound 1, 2 and 5) showed strong anti-HIV activity (EC50.064μg/ml), compound 3 and 4 showed less activity (EC50=6.18μg/ml and 12.85μg/ml respectively); Compound 5 and 6 showed weak cytotoxicity, and compound 1-4 showed moderate cytotoxicity; 2 Compound 5 and 6 were activated to all the four tested microbial; Compound 1, 2 and 13 were activated to all the three tested bacterial, but were unactivated to the tested fungi, Penicillium avellaneumUC-4376; One of the test bacterial, Staphylococcus aurues, seemed to be sensitive to all the eleven triterpenoids.

新化合物（化合物6）和化合物1、2、5具有强烈的体外抗HIV活性，其EC50值均小于0.064μg/ml，化合物3和4也具有抗HIV活性，但活性相对较弱，EC50值分别为6.18μg/ml和12.85μg/ml；化合物1-6都具有一定的细胞毒性，且化合物5和6的细胞毒性明显弱于化合物1-4的。 2）新化合物（化合物6）和化合物5对所有四种试验菌都有一定的活性；化合物1 、2和13对三种细菌均有一定的活性，但对真菌橙色青霉UC-4367（Penicillium avellaneum UC-4376）无活性；金黄色葡萄球菌对所有11个三萜都较为敏感。

The mc2155 carrying pMind-glmU-AS was induced by different concentration of tetracycline, and GlmU protein was detected in the mc2155 carrying pMind-glmU-AS by Western blotting. The results showed that tetracycline of 20ng/ml has obviously inhibited the growth rate of mc2155 carrying pMind-glmU-AS, but the amount of GlmU protein did not change compared to that of uninduced mc2155 carrying pMind-glmU-AS.

glmU反义RNA的诱导表达与GlmU蛋白的检测分别用5 ng/ml、10 ng/ml和20 ng/ml的四环素诱导携带pMind-glmU-AS表达载体的mc~2155菌株表达反义RNA，绘制细菌生长曲线，结果表明20 ng/ml浓度的四环素可抑制mc~2155/pMind-glmU-AS的生长，但Western Blotting分析结果显示经四环素诱导和未诱导的mc~2155/pMind-glmU-AS中，GlmU蛋白表达量无明显的变化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失