无菌

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

The optimum pH is 8-10 for hypha growth and 7-9 for conidiospore germination; The hypha could not grow on the culture medium which nitrogen source comes from urea and asparagines, but it could grow fast and produce more conidiospore on the culture medium which carbon source comes from maltose, glucose and lactose, and nitro0gen source come from glycine, histidiane and peptone.

该菌在PH 4~12范围内均能生长、产生分生孢子并萌发，菌丝生长的最适pH 8~10。，孢子萌发的最适PH 7~9；该菌不能在以尿素和天门冬酰氨为氮源的培养基上生长，但以麦芽糖、葡萄糖、乳糖、淀粉、甘氨酸、组氨酸和蛋白陈为碳源和氮源时，菌丝生长快、产孢量大；光照处理对该菌菌丝生长、产孢和孢子萌发无显著影响。

Steak mushroom bacteria, also known as tongue, liver fungus, pig tongue fungus, Japan said Velvet liver, blood Velvet, Mushroom soft flesh, juicy, red-brown, short-throw type or not, flavor concentration, can be used as medicine, the role of therapeutic blood.

牛排菇 又名牛舌菌、猪肝菌、猪舌菌、日本称肝脏茸、鲜血茸，菇肉质松软、多汁、红褐色、短柄式或无、香味浓，可以入药，食疗补血作用。

To Tai-keng No.9 and to Tai-keng No. 11. Besides, there is no relativity of the elements of the quantity, hypha growth rate and the radius of the distribution of sclerotium of test isolates pretend to be positive related.

另一方面、对於供试菌株中dsRNA出现的种类则与菌株的生理生化特性以及供试菌株接种三种水稻所获得之病害指数而言则也是无相关性。

Results The percentages of the four targeted bacteria in high-caries group were significantly higher than those in caries-free group (P.01), and there was good linear correlation between dmfs index and the sum of the percentages of Streptococcus mutans and Streptococcus sobrinus.

对各组所获数据进行统计学分析。结果高龋组中4种致龋菌所占总菌的比例均高于无龋组(P.01)，其中变异链球菌和无缘链球菌的数量之和所占有总菌的比例与dmfs指数具有良好的线性关系。

Significance and Impact of the Study: Vibrio alginolyticus is implicated in severe vibriosis of marine aquaculture systems although many strains are avirulent and could be used as probiotic strains.

本研究的意义以及影响：尽管许多溶藻弧菌是无毒菌株，甚至有的可以用作益生菌，它还是在水产养殖里引起了严重的弧菌病。

To test the antibacterial effect of MEBO against anaerobic spore-bearing bacillus, anaerobic non-spore-bearing bacillus (Bacteroides fragilis, propionibacterium acnes) and fungi, etc.

观察湿润烧伤膏对有芽胞厌氧菌、无芽胞厌氧菌(脆弱类杆菌、痤疮丙酸杆菌)和真菌等的抗菌作用。

The conidial production on infected sclerotia by the mutants SV-5- 1, SV-5-2, and SV-10-1 were as high as or higher than that by Chy-1 for at least one treatment with these mutants.

除菌株V-250-1外，其它3个突变菌株在至少1个接种处理的菌核上产生分生孢子的能力显著高于野生菌株或无明显差异。

Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus, Escherichia coli, Salmonella choleraesuis, Salmonella enteritidis and Salmonella typimurium were used as tested bacteria, the bacteriostasis was studied and the minimal inhibitory concentration was measured.

以金黄色葡萄球菌、无乳链球菌、蜡样芽孢杆菌、大肠杆菌、猪霍乱沙门氏菌、肠炎沙门氏菌及鼠伤寒沙门氏菌为供试菌，研究其抑菌活性，并测定其相应最小抑菌浓度。

The main pathogens of VAP were Gram negative organisms (68.63%) whose predominant pathogens were Pseudomonas aeruginosa and Acinetobacter. On the other hand, 75.40% of Gram positive organisms were Staphylococcus epidermidis.Of the pathogens 20.98% were eumycetes. Compared from June 1996 to June 2001 with from July 2001 to June 2006,there was no significant difference on the incidence rate of P.aeruginosa(31.76% vs 31.58%).During the recent 10 years,the incidence of Acinetobacter rose up from 8.23% to 15.23% and the rate of oxacillin-resistant Staphylococcus epidermidis rose up from 18.75% to 71.43%. The sensitivity of P.aeruginosa and Acinetobacter isolates descended to sodium imipenem cilastatin and ceftazidime in past 10 years.

在538例VAP中，2种及2种以上致病菌感染占56.51%；革兰阴性细菌中，铜绿假单胞菌和不动杆菌属为VAP的主要致病菌(68.63%)(385/561)；表皮葡萄球菌感染为VAP的主要革兰阳性致病菌(75.40%)(95/126)；真菌占20.98%(183/872)；铜绿假单胞菌在两个时间段所占比例无明显变化，不动杆菌属的检出率呈上升趋势(8.23%～15.23%)；铜绿假单胞菌和不动杆菌属对亚胺培南、头孢他啶的敏感性明显下降，耐苯唑西林表皮葡萄球菌的检出率显著升高(18.75%～71.43%)。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失