无菌

The columna soil study showed that strain NBT had the ablity of potassiumuptake ,making losed potassium from the soil inoculated with the bacterium wasdecreased by

通过土柱试验发现，硅酸盐细菌NBT菌株还具有保钾功能，供试土壤接菌处理后的土壤滤液中流失的钾比对照减少35.5-56.5％，经方差分析，差异达显著水平；另外，硅酸盐细菌NBT菌株与农药的相容性试验表明，无论在大田农药施用浓度和残留浓度内的丁草胺、绿黄隆、三唑酮粉、多菌灵、马拉硫磷等农药对NBT菌株没有抑制作用；乐果、甲基1605在大田施用浓度下，对NBT菌株有一定抑制作用，而在残留浓度下，对NBT菌株无明显抑制作用。

Preventing and controlling crown gall disease applied the early biological controls strain K84, itself did not have the pathogenicity, its plasmid may synthesize one kind of bacteriocin Agrocin84, may suppress many pathogenic strains, but did not have pathogenicity to non-pathogenic strain .

防治根癌病应用较早的生防菌株是K84，本身无致病性，它的质粒可以合成一种细菌素Agrocin84，可以抑制很多有致病性的菌株，而对非致病性的菌株则无影响。

Ammonifiers in Suzhou Creek can not use inorganic nitrogen and carbamide as nitrogen source; additional carbon source and garbage lixivium have little influence on ammonifiers growth; high content of salt and low temperature restrain ammonifiers growth; alkalescent condition has little influence on ammonifiers, but acidic condition restrain ammonifiers growth; the biomass of ammonifiers are not necessary correlated with the function of ammonifiers, adding glucose with 1g/l content into the water sample obviously promote the growth and function of ammonifiers.

苏州河的氨化菌无法利用无机氮和尿素作为氮源；在营养条件充足时添加额外C源和富含有机物的垃圾浸出液对氨化菌的生长基本无影响；高盐度和低温抑制氨化菌生长；碱性条件对氨化菌的生长影响不大，酸性条件对氨化菌生长具有抑制作用；氨化菌生物量的消长与转氨活性之间不存在必然联系，1g/L的葡萄糖对苏州河水样中氨化菌的数量和转氨功能具有明显的促进作用。

The morphological analysis of strain EMZY-1 showed that it was a straight rod, about 0.3×1.7 in size, and spherical and terminal spore appeared. Results of physiological and biochemical test showed that strain EMZY-1 was a Gram-positive bacteria, and could not grow when temperature was lower than 10℃ or higher than 60℃, pH was lower than 5.0 or higher than 12.0, and the concentration of NaCl was more than 10%. The strain EMZY could grow on the medium as a of sugar, glucose and mannite. The strain could use saccharose, glucose and mannitol as carbon resource, and NH4(superscript +) and NO3(superscript -) as nitrogen resource.

通过对该菌株的形态、培养特征、生理生化特征的研究以及16S rDNA序列分析表明：该菌株为革兰氏阳性菌；细胞为棒杆状，端生孢子，大小约0.3×1.7；温度低于10℃、高于60℃无明显生长；pH低于5.0、高于12.0无明显生长；NaCl质量浓度达到10%菌株不能生长；能在蔗糖、葡萄糖、甘露醇为C源的培养基上生长；NH4、NO3为菌株良好N源。

Result The heterobacteria, vibrios and pathogenic Vibrio parahaemolyticus were the roost in fertilized eggs of shrimp rot the least in nauplius, then their number would increase with growth. During whole rearing period, both heterobacteria in larvae, vibrios in water would increase by one order of magnitude, while both vibrios in larvae and heterobacteria in water would increase by two orders of magnitude.

结果］异养菌、弧菌和致病性副溶血菌数量都是对虾受精卵中高，无节幼体中最低，而后逐渐升高在整个育苗期，对虾幼体中异养菌和养殖水体中弧菌增加1个数量级，对虾幼体中弧菌和养殖水体中异养菌均增加2个数量级。

The results showed:among the 7 isolates, five isolates of HY3、GY1－3、ZJ1－1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示：7个菌株中，其中5个菌株HY3、GY1－3、ZJ1－1、HP1、FC3的菌落形态相同：不规则形状、流体、粘性、不透明、表面光滑；另外2个菌株93B、GN1菌落形态相同：不规则形状、干燥、不透明、表面粗糙；用7个菌株接种剪根桉树苗，发病情况非常明显，而自来水对照实验中桉树苗却不发病；无EPS菌悬液培养桉树剪根苗，发病率也很明显，但是相比原菌液，则发病率有不同程度的下降；从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株，用它们配制菌悬液培养桉树苗，设置剪根和不剪根两个处理，每个处理设置五个重复，数据分析结果显示：对于剪根苗，上部和中部、上部和下部的含菌量有显著的差异，中部和下部含菌量差异不显著；带根苗，上部、中部、下部含菌量彼此之间差异显著；不管是剪根苗还是带根苗，木质部和韧皮部含菌量之间的差异都非常显著；上中下与木韧交互作用中，含菌量差异非常显著；最后筛选出来的强弱2个菌株HY3和93B，它们的纤维素酶活性分别为：1.955ug/和1.288ug/，具有显著的差别；果胶酶的活性分别为：1.325 ug/和1.24ug/，没有显著的差别，而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著，分别为：7.08×10-8ug/cell和5.17×10-8ug/cell。

The antimicrobial test and researched was done on wide distillation of different solvents on Terpenoid, polarity separation of ethanol distillable substance, thin layer chromatography of different polarities and distillation of amylase. The results showed that the active substance distilled by 95% ethanol and V methanol: toluene=3:1 had stronger antimicrobial activity on Agaricus blazei Murrill. The antimicrobial activity of ethyl acetate was stronger than wide distilling substance with ethanol, but the water part has not antimicrobial effect. Two straps of ethyl acetate phase（Rf0.57、Rf0.63）(0.1g/ml)had stronger antimicrobial activity on Agaricus blazei Murrill, the antimicrobial zone on Pseudomonas was 19.3mm. The bacteriostatic activity of chromatography strap of water phase was rather weaker, the water solution (0.5g/g) of amylase had better effect on pathogenic fungus of Agaricus blazei Murrill, its antimicrobial zone on Aspergillus niger, Citrinin, Trichoderma viride were 10.8 mm, 7.9 mm, 5.3 mm.

通过对日本凹顶藻以不同溶剂的粗提、乙醇提取物的极性分离、不同极性的薄层层析和多糖的提取，进行抗菌试验的研究，发现95%乙醇和V甲醇：甲苯为3∶1的提取的活性物质具有较强的抗姬松茸的病原细菌活性，乙酸乙酯相对病原细菌的抑菌强于乙醇的粗提物，而水相基本无抑菌效果，乙酸乙酯相的两个带（Rf0.57、Rf0.63）浓度为0.1g/ml具有较强抗姬松茸病原细菌的活性，对假单孢杆菌抑菌圈达到19.3mm，水相层析带的抑菌活性较弱，多糖的粗提物配成0.25g/g的水溶液对姬松茸病原真菌有较好的抑菌效果对黑曲霉、桔青霉和绿色木霉的抑菌圈分别达到10.8 mm、7.9 mm、5.3 mm。

The antimicrobial activities were determined with paper disc diffusion method. The result showed that of Sapindus mukorossi extracts only inhibited Candida albicans and no antimicrobial activities to Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pityrosporum ovale and Propionibacterium acnes.

配制无患子溶液进行抗菌试验，以圆盘滤纸扩散法分析，结果显示甲、乙、丙、丁醇溶液样品，唯对白色念珠菌具抑制效果，而对另外5种菌种(大肠杆菌、金黄色葡萄球菌、绿脓杆菌、皮屑芽孢菌、痤疮杆菌)无明显抗菌效果。

The third year we plan to isolate, purify and analize the active compounds which are evaluated by the analysis of bio-assay according to former system. However, the correct scientific species of common name is still confused since the ancient time peoples did not have the taxonomic system.

桑黄在古代并无正确的「分类」观念，所以代代相传，对於长相相似的菇类也被当成桑黄使用，是故今日虽然知道桑黄的属名确定为担子菌纲、多孔菌目、层孔菌属，但对於真正的种名仍无定论。

In litmus milk experiment, all of strains produced alkai expect for MZ02 and MZ04 produced acid reactions. At 30℃, all of strains grew best, and had a wide range in salt, acid and basic resistance. MZ04 was capable of good growth at salt density of 2.0%, MZ04 and MZ10 could still grew better at pH 9.0. All of the strains were not resistant to 250 μg /mL gentamicin(Sm3), and to other antibiotics, different resistances were presented by different strains, MZ02 had worst resistance to all antibiotics, while MZ04, MZ08,MZ09 had strong resistance.

所有供试菌株在温度30℃时生长最好，耐盐、耐酸碱范围较宽，MZ04在含盐浓度为2.0%环境还能生长良好，MZ04、MZ10在pH9.0环境仍能生长较好；所有菌株对250 μg /mL 庆大霉素（Sm3）均无抗性，不同菌株对其余供试抗菌素表现出不同的抗性，MZ02抗性最差，对所供试的抗生素均无抗性；MZ04、MZ08、MZ09抗性相对较强。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失