无菌

Results: Fresh nHA-PA66 paste and polymerized nHA-PA66 films showed no antimicrobial activity to Sm and little to Av and Lc. After mixed with the iodoform, the antimicrobial activity of nHA-PA66/I paste obviously increased, but no significant difference between nHA-PA66/I and CH. There was no antimicrobial activity of HA to the three test bacterial strains.

结果：抗菌实验结果表明，nHA-PA66固化膜片与nHA-PA66糊剂对Sm无抑菌作用，对Av和Lc有极轻微抑菌作用，膜片与糊剂之间无显著性差异；加入碘仿后的nHA-PA66糊剂抑菌能力显著增强，与氢氧化钙相比无显著性差异，常规羟磷灰石对三种实验菌均无抑菌作用。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

Results: The ranges of MIC of DNA-2E on trichophyton rubrum and beard-like trichophyton were 0.0625~0.125mg/mL and 0.03125~0.125mg/mL respectively; of which there was no significant difference between the two fungi. Both 2% and 4% DNA-2E had better effects on ringworm of guinea pig in feet and body infected by beard-like trichophyton, which showed significant difference compared to distilled water and no significant difference compared to 1% Zuguang powder. Both 4% and 6% DNA-2E had better Antipruritic effect, which showed no significant difference compared to 2% Zuguang Powder and significant difference compared to distilled water.

结果：癣清(DNA-2E)对红色毛癣菌、须状毛癣菌的最低抑菌浓度范围分别为0.0625~0.125mg/mL和0.03125~0.125mg/mL，两组比较差异无显著性；2%和4%癣清(DNA-2E)对豚鼠须状毛癣菌感染足癣、体癣有较好疗效，与1%足光粉比较差异无显著性，与蒸镏水组比较差异有高度显著性；4%和6%癣清(DNA-2E)止痒作用与1%足光粉相比差异无显著性，与蒸镏水组比较差异有高度显著性。

The primers and the detection programs have been used to detect the pathogens in the inoculated-diseased cucumber plants .The details are reported as follows: 1. By using the primers ITS1/ITS4, only Fusarium isolates could be amplified to produce a special fragment of 750bp. This product generated 480bp, 200bp and 100bp fragments after digested by restriction enzyme HinfⅠonly from the pathogen of Cucurbits Fusarium Wilt.

引物ITS1/ITS4只在镰孢属菌株上扩增到一条750bp分子片段，其他供试菌株无扩增条带产生，扩增产物经HinfⅠ酶解后，只有瓜类尖镰孢菌产生480bp、200bp和100bp 3条带，说明该PCR-RFLP程序对瓜类尖镰孢菌具有特异性；巢式PCR引物BIK1/BIK4和BIK2/BIK3只有在黄瓜尖镰孢菌菌株上扩增到一条800bp分子片段，其他菌株均无扩增条带产生，说明该巢式PCR程序对黄瓜尖镰孢菌具有特异性。

The compatibility of both Gvirens and B.firmus was researched. The fermentation filtrate of Gvirens , under the concentration of 70%, had not distinct inhibitory activity to B.firmus, the biomass of B.firmus would decrease with above the 70% filtrate of Gvirens, but the fermentation filtrate of B.firmus had not inhibitive role to Gvirens. 7, The cooperation control of both Gvirens and B.firm to the pine needle blight was explored:(1) Pathogen inoculation happened prior to the bio-control treatment for 7 days, however, the disease index and control effect had not distinct differences in any treatments even if combination of the two antagonists, but the susceptibility for different kind of pine were different.(2)when antagonist and pathogen were inoculated at the same time, some control effect to the pine needle blight, including certain extent cooperation control of both Gvirens and B.firm were proved, but it wasn't distinct when two antagonists used separately, and the change of the antagonist concentration had little effect to the control effect.

G.virens与B.firmus对松赤枯病的协同控制显示：(1)预先接种病菌后(7天)再进行生物防治处理，不管那种情况，感病指数和防治效果在处理间差异不明显，浓度影响无显著差异，即使是两种生防制剂联合作用也未显著提高其防效，但不同树种间的感病性有明显差异；(2)领抗菌与病原菌同时接种，对松赤枯病有一定防治效果，两菌联合有一定的协同作用，两菌分别单独施用防效差异不显著，浓度变化对防效影响不大；(3)预先接种颇抗菌能显著提高防治效果，两菌联合处理防效更优，感病树种的感病指数可降至13一15，抗病树种的感病指数可控制在3%左右；在同一浓度下B.f1'r功US、叹叮厂即s间无显著差异，在同一领抗菌下，浓度愈高，防效愈高：松，(4)三种生防接种方式的平均效果分析表明，叹F行ens最高平均防效49.5%黑 B。

The results show that though the strain is growing slower in the nitrogen-free medium than in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium. The capsular polysaccharides produced by the strain in the culture which contains different mineral powders will reach the highest content on the third or the fourth day in its growing period. The strain's capability of releasing potassium from k-feldspar and biotite in the nitrogen-containing medium is higher than that in the nitrogen-free medium because in the nitrogen-free medium, the strain and its production of glucoprotein are less than those in the nitrogen-containing medium.

结果表明，尽管该菌在无氮培养条件下的菌体数量远小于有氮培养条件，但无氮培养条件下所提取的细菌多糖多于在有氮条件下培养所提取的细菌多糖；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的无氮培养基中生长可形成大量多糖，采用丙酮法进行细菌培养液多糖提取，发现细菌培养的第3天所提多糖含量最高；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的有氮培养基中生长亦可形成较多的多糖，且在细菌培养的第4天所提多糖含量最高；胶质芽孢杆菌在有氮条件下对含钾矿物的释钾率高于在无氮条件下的释钾率，这可能与该菌在有氮条件下生长更快、可产生较多菌体细胞有关。

Ninety seven spores or sporocarps of VAMF were wet sieved from the rhizosphere soil of mycorrhized pteridophytes, from which 25 VAMF species which belonged to the genera of Acaulospora, Gigaspora, Glomus, Sclerocystis and Scutellospora respectively were identified, and the taxonomic characters of the 25 species were described. The Glomus fungi were the dominant VAMF in the rhizosphere soil of pteridophytes, they took up 44% in the identified 25 VAMF (11 species belong to Glomus); the fungi in Acaulospora and Sclerocystis in the next place, they took up 24% and 16% respectively in the identified VAMF; the fungi in the other genera took up 16%. In terms of the relationships of pteridophytes and the VAMF isolated from their rhizosphere soil, there was no specifity between the VAMF and their host.

从具有VA菌根的蕨类植物对应的根际土壤中用湿筛法筛取了97份VA菌根真菌的孢子或孢子果，从中鉴定出了分属于无醒囊霉属、巨孢囊霉属、球囊霉属、硬囊霉属和盾孢囊霉属的VA菌根真菌25种，并对这25种真菌的分类学特征进行了描述；球囊霉属真菌是蕨类植物根际土壤中的优势类群，已鉴定的25种真菌中，有11种属球囊霉属真菌，占44%，其次是无梗囊霉属和硬囊霉属真菌，分别占24%和16%，其他属的VA菌根真菌占16%，从分离鉴定的蕨类植物根际土壤中的VA菌根真菌与蕨类植物的关系看，VA菌根真菌与蕨类植物之间无明显的专一性。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系：选取生长20d左右的健壮的小黑杨组培苗叶片，通过主叶脉剪2～3下，预培养3～4d；将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3～0.4浓度，进行浸染；侵染时间为6～15min；共培养3d后，转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

On November 19, 1941, the Sydney was three days from port, about 240km south-west of Carnarvon in Western Australia.

八天以后，奥斯丁博士，一位专门从事从外来及早已死亡生物的基因物质上捕捉秘密的专家，做着进入南半球最好的无菌实验室的准备。

S ufa."We want to create a sterile area between the crossings that would reduce the risk of car bombs and other attacks against the crossings," said a defense official working on the project.

&我们要建立一个无菌区之间的通道，这将减少风险，汽车炸弹和其他攻击事件，对口岸，说：&国防部官员参与该项目工作。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。