无菌 - 网络例句 - 英语人，englisher

METHODS: Human amnion membrane derived mesenchymal stem cells were sterilely isolated and cultured. The third passages of cells (1×109/L)(0.5 mL) were transplanted by tail venous pathway to mice. Mice in the control group were injected with an equal volume of saline, whereas mice in the normal group were left intact.

无菌条件下体外分离培养人羊膜间充质干细胞，传至第3代将细胞浓度调整为1×109 L-1，经尾静脉注入0.5 mL至细胞移植组小鼠体内；对照组经尾静脉注入同体积的生理盐水；正常组小鼠不给予任何干预措施。

ICR fetus mice were sterilely obtained to culture mouse embryonic fibroblasts by tissue pancreatin digestion method.

无菌条件下取出ICR胎鼠，组织块胰酶消化法分离培养小鼠胚胎成纤维细胞，接种在0.1%明胶包被的6孔板中即为饲养层。

METHODS: Fasting venous blood of participants in two groups was harvested sterilely at 6 months and 1 year after test group restoration, serum nickel and chromium content were determined using graphite furnace atomic absorption spectrometry.

无菌抽取对照组健康者及实验组患者修复后0.5年，1年空腹静脉血，采用石墨炉原子吸收光谱法测定血清中镍、铬元素的含量。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

Five male Wistar rats of 4－5 weeks old were executed by cutting off the heads to sterilely obtain the muscle group in hind limbs, remove the fat and the fascia, so as to prepare for single cell suspension.

选取4～5周龄Wistar雄性大鼠5只，脱颈处死无菌切取后腿肌群，剪除脂肪和筋膜，制备单细胞悬液。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

Methods Human umbilical cord blood samples were obtained sterilely from healthy parturients. MSCs were isolated by lymphocyte separation medium (density 1.077g/ml), MSCs were obtained by adhere culture. The surface antigen expression of MSCs was detected by flow cytometry.

将无菌条件下采集健康产妇脐带血，用相对密度为1.077g/ml的人淋巴细胞分离液分离脐血单个核细胞，利用间充质干细胞贴壁生长的特性获得MSCs，流式细胞仪检测其表面抗原标志。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。