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Rat mesencephalic ventral brain tissue was isolated for culture of neural stem cells.

①分别于大鼠孕10,12,14,16,18天摸球法随机选取6只,麻醉后处死孕鼠,无菌条件下剖腹取胚胎,分离中脑腹侧脑组织进行神经干细胞培养。

Researchers started with mice that were specially bred to be germ-free - with no gut microbiota of their own - and to be able to nurture human gut microbiota instead.

研究中使用了通过特殊饲养技术获得的无菌小鼠。这种小鼠可以接受人类的肠道菌类在其肠道中繁殖。

Although the microporous structure made it infected easily, it could be prevented by strictly sterile operation.

虽然多孔结构使其容易造成感染,但通过一系列严格的无菌操作可以有效地预防此类并发症的发生。

METHODS: Neonatal mouse brain tissues were sterilely obtained to prepare monoplast suspension.

无菌条件下取新生小鼠脑组织,制备单细胞悬液。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏,以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液,分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心,吸取界层细胞,添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

Viscous oils may be diluted as necessary with a suitable sterile diluent such as isopropyl myristate shown not to have antimicrobial activity in the conditions of the test.

如需要,粘稠油质可以用适合的无菌稀释剂进行稀释,例如已证实在该试验条件下不具有抗微生物活性的豆蔻酸异丙酯。

In the process, macrophages play major roles which could get rid of the necrotic tissues and secrete nutrition factors.

既往观点认为,中枢神经系统的无菌性炎症反应是有害的,尤其对于SCI后的反应。

Make sure to also pack a good amount of sterile gauze pads and nonadherent pads so the wound doesn't heal to the dressings.

同时确保带上大量无菌纱布垫子和还没粘起来的垫子以应付需要包起来才能痊愈的伤口。

I am sure this is said for bacterial prostatitis, I don't think that nonbacterial should make any different regarding sex.

这一点对于细菌性前列腺炎绝对是正确的,我并不认为无菌性前列腺炎会有什么不同。

Abstracts:objective To study the function of fibroblasts in nonbacterial loosening.

目的 探讨成纤维细胞在人工关节无菌性松动中的作用。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

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