无菌

Methods Under aseptic condition, mechanically blocking of the hepatoduodenal ligement was adopted in rabbit model, both endotoxin level of the portal blood and positive bacterial cultures in the mensenteric lymph nodes were observed at 15, 20, 30 and 60 min respectively after the blocking.

采用兔动物模型，在无菌条件下机械性阻断肝十二指肠韧带，观察阻断后15，20，30min和60min时门静脉血中内毒素值和肠系膜淋巴结细菌培养阳性数。

Methods Hepatoduodenal ligament was blocked under normal temperature and aseptic condition in rabbits model to lead to an intestinal anoxia and damage of intestinal mucosa barrier, and then a great quantity of endotoxin would enter portal vein through intestinal wall resulting in endotoxinemia.

采用兔做动物模型，在常温无菌条件下机械性阻断肝十二指肠韧带造成肠道缺血、缺氧、肠黏膜屏障损害，肠道内大量内毒素穿过肠壁进入门静脉，造成内毒素血症。

Hermo Gasifier Ecological Technology is an innovative, low-cost method for treating domestic waste -- and in the process transforming it into a sterile ash, and fuel gas which can be used for generating electricity.

GE Tech 报告是一种创新的，低成本的方式处理废物包括--在此过程中，将废物转化成无菌的灰，燃料汽油用来发电。

The iron plate that will be equally big or small with the flat instrument even welds seven short carefully steel needles,and the structure into scissors or pliers welds respectively 2 handles welding seven apertures on the brill used equally big or smalllythe iron plate again andcorrespondence,highhandedly during the use exterminates bacterium first,then inserts the quick scraps of paper of the medicine selecting to the steel nail,with iron piece and the culture dish to neat,pinching the scraps of paper falling inside the culture dish with the hand,namely the tweezers are pressed passablely slightly with the asepsis.

将与平皿同样大小的铁板均匀的焊七个短细钢针，再用同样大小的铁板钻七个小孔与之对应，分别焊2个柄焊成剪子或钳子的结构，使用时先高压灭菌，然后将所选药敏纸片插到钢钉上，将铁片和培养皿对齐，用手一捏纸片落入培养皿内，用无菌镊子稍压即可。

MATERIALS AND METHODS: Bilateral hippocampi were isolated from neonatal Wistar rats; cellular suspensions were prepared under sterile conditions.

材料与方法：取新生Wistar 大鼠双侧海马，无菌条件下制备细胞悬液。

Objective To study the protective action of compound agent madeup with effective ingredients of Hirudo and Rhizoma chuanxiong and Folium Ginkgo in animal ischemic cerebral infarction.Methods To mix Hirudin extracts of Folium Ginkgo and Ligustrazine according2:25:5into asepsis injection,rats local cereˉbral ischemia induced by embolism of middle cerebral arterywas used as experimental model to study the efˉfects of the compound agent and positive control drug,Nimodipine and extracts of Folium Ginkgo on cerebral infarct size and neurological deficits.Disposing the experimental data with statisticsand comparing their otherness among difˉferent groups.

目的 探讨用中药水蛭、银杏、川芎的有效成分组成的复方制剂对动物局灶脑缺血的保护作用方法将水蛭素、银杏叶提取物、川芎嗪按2∶25∶5的比例制成无菌注射液，应用鼠大脑中动脉栓塞导致缺血性脑卒中的实验模型，并用生理盐水、尼莫地平和银杏提取物作对照，观察不同药物对脑缺血面积和由缺血造成的大鼠行为障碍的影响，将实验据用统计学处理并进行组间的差异性比较。

Methods: The model of multi-infarction dementia was made by means of retrograde catheterization infusing embolus, which was made of airdry sterilized blood clot of homogenic rats, to arteriae carotis externa.

取同种大鼠无菌自然干燥血凝块研碎，于颈外动脉处逆行插管注入栓子制作多发性梗死性痴呆动物模型。

METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤，将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮，再置入曲拉通X-100溶液使真皮细胞完全脱净，磷酸盐缓冲液反复清洗，即为脱细胞真皮基质，4 ℃保存备用。

Cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤，将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮，再置入曲拉通X-100溶液使真皮细胞完全脱净，磷酸盐缓冲液反复清洗，即为脱细胞真皮基质，4 ℃保存备用。

Methods Thirty eight patients with loosening of hip prosthesis were divided into three group: total hip arthoplasty, double hip arthuplasty and integrated arthoplasty. The inflammative reaction was analyzed with light microscopic observation arrording to the half-ration standard of Joseph, and the content of TNF in samples of interface membranes was determined.

方法］根据假体的类型及假体中是否含有聚乙烯将38例人工髋关节翻修病例分为3组：全髋置换组、双动头组和一体式单动头组，对各组术中松动假体周围界膜组织进行光镜下炎性细胞计数的半定量分析和肿瘤坏死因子的测定，分析人工髋关节无菌性松动与聚乙烯微粒、细胞因子之间的相关关系。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。