无菌

Salina were obtained, and axenic status was confirmed by different methods.

经过不同方法检测，确证该5藻株属无菌藻株。

In this thesis, the biological characteristic of Dunaliella salina, which included the axenic purification, physiology characteristics and genetics characteristics, was studied systematically.

本文系统研究了杜氏盐藻的生物学特性，主要内容包括杜氏盐藻无菌纯化、杜氏盐藻生理学特性、杜氏盐藻遗传学特性等三部分。

Three types of initial explants from Tilia miqucliana, including stem of seedlings, leaf and root of axenic plantlet, were used to study the organogenesis in this thesis.

本研究以南京椴无菌试管苗的茎段、叶片、根系为外植体，进行器官发生研究，探讨了不同因素对南京椴直接器官发生以及愈伤组织诱导的影响。主要结果如下： 1。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The contents of caecum and rectum of 30 healthy broilers were collected by aseptic method.

无菌采取30只健康鸡的盲肠、直肠内容物，以大肠杆菌O_8为宿主，从这些样品中分离到一株能裂解其它细菌的细菌。

Since then, people have made many studies on resorbable internal bone fixation composites. Up till now, however, although those kinds of resorbable internal fixation composites have considerable strength, they can only be used for curing cancellated bone fracture and not for load-bearing bone fracture internal fixation because of their low modulus, too slow degradation rate and inflammation reaction.

然而到目前为止，可降解吸收性骨内固定材料，虽然具有一定的强度，但因刚度低，故在临床上仅用于治疗松质骨骨折，尚不能用于承重骨骨折的内固定，且在体内降解吸收时间太长以及容易引起无菌性炎症反应等，使其应用范围受到限制。

Every complication has its pathologic and clinical characteristics.Conclusion Many measures are important for reducing complications including strict surgical manipulation, proper selection of good implants, preferred submuscular insertion, whole capsulectomy for prosthesis rupture or leak and through hemostasis, proper bandage, massage and follow-up et al.

严格执行无菌操作、熟练掌握该术技能、选择优质假体、首选胸大肌下置入、对假体破裂或渗漏而再次手术者须尽量完全切除包膜并彻底止血、术后正确包扎按摩以及术后重视随访监测等综合措施，是避免和减少隆乳术后并发症的关键。

The apical buds of Celosia cristata were used as the explants for the rapid propagation to compare the mutagenic effects treated with colchicines at different concentrations and time duration in tissue culture.

以鸡冠花顶芽为外植体，研究其离体快繁程序并在无菌体繁殖条件下探索了秋水仙素溶液不同浓度和时间处理对鸡冠花的诱变效应。

Emphasis shall be put on the key technology such as the membrane separation, supercritical exaction, clasmatosis, micro capsule embedding, microwave, asepsis processing and packaging of the modern food industry, the new production technology of high-tech and high-value-added new type chemicals for daily use, household electrical appliance, lighting electrical equipment, battery, ceramics, plastic, machines for daily use, and the clean production technology and pollution treatment technology for the industries of paper making, leather, ferment and food.

要重点发展现代食品工业生产的膜分离、超临界萃取、细胞破壁、微胶囊包埋、微波、无菌加工和包装等关键技术；先进造纸技术；高技术含量、高附加值的新型日用化工、家用电器、照明电器、电池、陶瓷、塑料、日用机械等新产品生产技术；造纸、皮革、发酵和食品行业的清洁生产技术、污染治理技术。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。