无菌

While in this study, 2 week old Kunming mouse were killed by cervical dislocation. The long bone was collected on the super clean blench through sterile method, then periosteum and epiphyses were discarded.

另外，试验采用2周龄的昆明小鼠，拉颈处死，在超净工作台内无菌分离长骨，剔除骨周组织和骨骺端。

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment.

试验采用24h内昆明乳鼠，拉颈处死，无菌取出头盖骨，在超净工作台内将其剪碎，先用0.25%胰蛋白酶37℃消化30min，然后用0.2%胶原酶37℃振荡消化60min，反复吹打骨片分离更多细胞。

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment. The bone pieces were digested by 0.25% trypsin for 30 min at 37℃ at first, then digested by 0.2% collagenase at 37℃ in constant temperature oscillator for 60 min, and rinsed repeatedly to disaggregate more single-cells.

试验采用24h内昆明乳鼠，拉颈处死，无菌取出头盖骨，在超净工作台内将其剪碎，先用0.25%胰蛋白酶37℃消化30min，然后用0.2%胶原酶37℃振荡消化60min，反复吹打骨片分离更多细胞。

We've got an open body cavity in a barely sterile environment.

这里是非无菌环境有个人开膛破肚地躺着

Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.

石蒜属植物快速繁殖技术研究中，在无菌无性系建立阶段：鳞茎以0.1％HgCl_2消毒10-12分钟效果最好，而叶、根尖、鳞芽等较干净部位选用0.1％HgCl_2消毒7分钟；用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养，以带基盘鳞片诱导分化效果最理想，鳞芽易培养出芽，但数量有限，而叶片、根尖与不带基盘鳞片均未诱导分化；带基盘鳞片为石蒜属植物快速繁殖最佳外植体，以鳞茎中部3-15层芽诱导率高，较外层稍次之，内部鳞片诱导率最低；选用八等分法、六等分法、四等分法切割鳞茎，六等分法综合效果最好；每外植体带三鳞片培养最为适宜；三种石蒜属植物各自适合的培养基成分不同，换锦花在MS+BA5mg／l+NAA0.1mg／l中培养效果最好，夏水仙为MS+BA5mg／l+NAA0.5mg／l，而白花长筒石蒜在各培养基组合中培养效果均不理想，其适合的培养条件有待于进一步研究。

When scales are proposed by nutrition solution, the information of bulblet is very regular: information of bulblet is almost located in inside 1—2mm of wound, the root is formed below of bulblet.

营养液浸种鳞片，小子球的分化很有规律性，据试验观察，小子球发生大都在伤口腹内侧1—2mm处，在沿切口呈线状排列，腹外侧和愈伤组织处只占1%左右，小子球形成后多在其下部生根；而一般基质无菌水培养的多先在鳞片下部生根，后形成小子球，即较前者推迟一周。

We invest the large amount of money, has achieved the gelatin production channelization, the insulate the organizational management system, the automation truly and aseptic.

我们投入巨资，真正做到了明胶生产的管道化、封闭化、自动化和无菌化。

This course focuses on the foundation for chirurgery, common surgical diseases, surgical operations, etc. The objectives of this course are 1 to help students to master the basic theories, knowledge and techniques, the causes, pathogenic mechanism and clinical manifestations of common surgical diseases, and the principles of diagnosis, treatment and prevention of the diseases, 2 to help them get familiar with the indications and contraindications; and 3 to train students of the basic surgical performances outside the classroom and foster their aseptic concepts.

本课程包括外科基础、外科常见疾病、外科手术学等内容，使学生掌握外科学基础理论、基本知识、基本技能；掌握外科常见疾病的病因、病理、临床表现、诊断、鉴别诊断、预防和治疗原则；熟悉手术的适应症和禁忌症，训练学生外科手术学基本操作和培养学生严格的无菌观念。

Methods: Under sterilization, swines were opened chest and ligated left circumflex coronary artery.

无菌条件下开胸结扎猪冠状动脉左回旋支，建立AMI动物模型。

His immune system is shot. We need to get him into a clean room.

他的免疫系统亢奋我们需要把他带进无菌室

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。