无菌

The results showed that the bacteria B7 came into the logarithmic growth phase for 5 h culture. Both the fermentation liquid and its filtration could inhibit C. colhounii effectively, and the fermentation of dilution l0 times also showed significant differences compared with the control. The inhibiting effect of its fermentation media filtration was even more significant in the optimizing test, with the maximal inhibition rate of 100%. The best fermentation condition was 0.5% glucose, 0.1% ammonium muriate, without extra mineral, pH 5.4, temperature 28℃. Based on the morphological properties and 16S rRNA sequence, the bacterium B7 was identified as the Bacillus subtilis.

结果表明，该菌体在培养了5h后即进入对数生长期；B7细菌的发酵液原液和无菌滤液均能抑制病原菌的生长，其中发酵液原液的10^3稀释液的抑菌效果仍与对照之间存在显著的差异；发酵条件优化试验中发现该细菌的无菌滤液最大抑菌率达100%，最佳发酵条件为葡萄糖0.5%、氯化钱0.1%、不加无机盐、pH5.4，温度28℃；结合形态学特征和16S rRNA的序列分析，将该菌株鉴定为枯草芽孢杆菌。

Devices delivered in a sterile state must be designed, manufactured and packed in a non-reusable pack and/or according to appropriate procedures to ensure they are sterile when placed on the market and remain sterile, under the storage and transport conditions laid down, until the protective packaging is damaged or opened.

无菌器械的设计、生产和包装应采用一次性使用包装方式，并且在一定工作程序下保证器械上市时处于无菌状态，在贮藏、运输条件下只要包装不破损或开封，能够保持无菌状态。

Its principal use has been for surface sterilization of powders as a precaution against potential microbiological contamination of the sterile powder during aseptic handling.

乙撑氧也被用在无菌粉末的处理上，它的主要用途是对原粉表面灭菌，在无菌处理期间防止对无菌粉末可能造成的微生物污染。

One, suck phlegmy the preparation before works 1 should make sure negative pressure attracts plant above all each conduit joins correct, close together, unobstructed, assure significant negative pressure, general adult 10.64~15.96Kpa, the baby should be controlled in 7.98~10.64Kpa. 2 preparation rinse the asepsis physiological saline that smokes phlegmy pipe 2 bottles, 1 bottle wash pump technically with Yu Chong the suction inside tracheal spile is phlegmy canal , additional 1 bottle rinse those who had sucked mouth, nasal cavity technically to smoke phlegmy pipe . 3 equipment are very different the asepsis of model sucks phlegmy canal and asepsis glove. 4 because suck phlegmy itself,be a kind of stimulation to the patient, sober patient is not willing to suck normally phlegmy, and the family member of insensible patient also thinks to suck phlegmy special anguish.

一、吸痰前的预备工作1首先要保证负压吸引装置各管道连接正确、紧密、通畅，保证有效的负压，一般成人10.64~15.96Kpa，婴儿应控制在7.98~10.64Kpa.2预备2瓶冲洗吸痰管的无菌生理盐水，1瓶专门用于冲洗抽吸气管插管内的吸痰管，另1瓶专门冲洗吸过口、鼻腔的吸痰管。3备好不同型号的无菌吸痰管及无菌手套。4由于吸痰本身对病人是一种刺激，清醒的病人通常不愿意吸痰，而昏迷病人的家属也认为吸痰非凡痛苦。

We believe it essential to recognize the validity of zero as a target but at the same time remind industry and the regulatory community that this target may not be consistently and uniformly attainable because no aseptic environment or aseptically produced product is provably sterile.

我们相信，承认验证零污染这一目标是至关重要的；但与此同时，我们想提请制药公司和药证机构注意的是，这一目标可能不会持续地并广泛地被保持–因为没有任何无菌环境或是无菌生产的产品可以被证明是无菌的。

Eggs of axenic Bursaphelenchus xylophilus were kept on the colonies of various strains, and the results showed that on slanting cultures of certain strains, the eggs hatched, developed and reproduced well, i.e.

将无菌松材线虫卵置于各细菌菌株的菌苔培养的试验结果表明：无菌松材线虫卵能在某些斜面培养的细菌菌株的菌苔上孵化、生长、繁殖，即仅仅依靠细菌或其提供的营养，无菌松材线虫就能完成其生活史。

Storage of drug products after release;(9) Control and laboratory operations;(10) Aseptic processing, which includes as appropriate: I Floors, walls, and ceilings of smooth, hard surfaces that are easily cleanable; II Temperature and humidity controls; III An air supply filtered through high-efficiency particulate air filters under positive pressure, regardless of whether flow is laminar or nonlaminar; IV A system for monitoring environmental conditions; V A system for cleaning and disinfecting the room and equipment to produce aseptic conditions; VI A system for maintaining any equipment used to control the aseptic conditions.

无菌操作及有关要求： I 地板，墙壁和天花板平滑，坚硬，表面易清洁； II 温度与湿度控制； III 空气经高效过滤器，在正压下过滤，层流或非层流均可； IV 环境检测系统； V 创造无菌环境，房间和设备的清洁，消毒系统； VI 控制无菌环境的设备维修系统。

Seeds of Pinus radiata were mixed with the finely divided sporophores in water.

由孢子包裹的种子发育成的苗木，形成许多须腹菌外生菌根在无菌土及缺乏外生菌根菌的非无菌土中。

Domestic companies to research and development of sterile, fresh packaging, revitalize national industry as its mission, has successfully developed more than 20 years of aseptic packaging 5-6 Composite paper, house-shaped packages, brick-shaped liquid, pincushion, housing shape, Triangle, ax-shaped drinks in aseptic packaging machine and ancillary support equipment, aluminum and Zhisu multi-storey complex in the printing paper, punching, indentation, composite, at all, the whole election, such as complete sets of equipment.

公司以研究开发国产化无菌、保鲜包装，振兴民族工业为己任，20多年来先后研制成功5—6层无菌包装复合纸、屋形包，液体砖形、枕形、屋形、三角形、斧形饮料无菌包装机以及相配套的辅助设备，和纸塑铝多层复合纸的印刷、冲孔、压痕、复合、分切、整选等成套设备。

Besides,the control of production techniques and the key techniques including sterile filtration and sterile bottle-filling etc.

主要叙述了纯生啤酒的特点，进一步阐述了纯生啤酒生产过程中如何控制生产工艺、无菌过滤和无菌灌装等重点工序，严格实现无菌化操作，从而实现生产出优质的纯生啤酒。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失