无荚膜的

In this paper, we have studied the content of the capsular polysaccharides of Bacillus mucilaginosus KO1 and the capability of the bacterial potassium-releasing from K-feldspar and biotite in the medium containing nitrogen or free of nitrogen.

研究了胶质芽孢杆菌KO1菌株在不同培养条件下，其荚膜多糖含量随时间的变化以及菌株在有氮、无氮培养基中的解钾作用。

The results showed that the strain HH407 was gram negative, rod shaped, aerobic, with a single polar flagellum, neither endospore nor capsule formed, about 0.4 ～0.7 μm×1.0～1.8 μm in size.

对从连云港海域筛选的一株产低温碱性蛋白酶的海洋适冷菌HH407进行的鉴定和生长特性研究结果表明，该菌为革兰氏阴性杆菌，好氧，端生鞭毛，无芽孢及荚膜，大小为0.4～0.7μm 1.0-1.8μm，在酪蛋白培养基的菌落特征为椭圆金黄色、光滑、半透明、四周隆起。

A strain HH407 which could production cold-active alkaline protease, was isolated from the sea area of Lianyungang. Then, the identification and physiological characterization of the strain HH407 were studied. The results showed that the strain HH407 was gram negative, rod shaped, aerobic, with a single polar flagellum, neither endospore nor capsule formed, about 0.4~0.7 μm×1.0~1.8 μm in size. The colony on screening medium were circular, yellow color, smooth, translucent, convex with regular edges.

对从连云港海域筛选的一株产低温碱性蛋白酶的海洋适冷菌HH407进行的鉴定和生长特性研究结果表明，该菌为革兰氏阴性杆菌，好氧，端生鞭毛，无芽孢及荚膜，大小为0.4~0.7μm×1.0-1.8μm，在酪蛋白培养基的菌落特征为椭圆金黄色、光滑、半透明、四周隆起。

So, we supposed that there is possibly a positive ion channal existed in the capsular layer.

将胶质芽孢杆菌放置在有氮或无氮培养基中培养，检测其荚膜多糖含量的变化。

The spermatozoa are embeded in a extracellalar matrix composed of high dense fibrils matrix and flocculent and concentric vesicles similar to that in the primary spermatophore layer, but the later lack of spermotozoa. The flocculent and concentric vesicles are often seen to release their contents around the main body of spermatozoa. The spermatozoa may absorb the contents to form vesicular zone and membrane zone in sperm nucleu. The secondary spermatophore layer is distinctly subdivided into inner region and outer region. The loose outer region consists of canaliculated reticulum and large flocculent vesicles.

输精管内的精荚为管状，由精子群、精荚基质及初级精荚壁和次级精荚壁组成，初级精荚壁与精荚基质是相连续的，它们之间无明显界限，两者均由电子密度较高的纤丝状基质和絮状泡和同心圆泡组成，但后者不含精子，后者中的絮状泡和同心圆泡常在精子主体部周围释放内含物，可能与形成精子细胞核中的泡状带和膜状带有关，在交配后的雌虾，其头胸部腹面上粘着的精荚中，其基质内已不见絮状和同心圆泡，仅见稀疏的纤丝。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。