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Methods After brain death of the donor was confirmed, the whole donor arm was sterilized with 3%iodine tincture and 75%ethyl alcohol and strapped with sterile towels. A circular incision 5 cm above the elbow was made, and the brachial artery was dissected and catheterized for perfusion of UW solution at 4℃. The arm was amputated with the brachial artery still in continuity and catheterized.

方法在供者被宣布脑死亡后,将拟取肢体用3%碘酒、75%酒精常规消毒,从肘关节以上5 cm平面作环形皮肤切口,游离肱动脉后插管,用4℃ UW液灌注后行前臂离断,再灌洗至回流液清亮后拔管,肢体作标记后用无菌敷料包裹、塑料袋封装,装入放置冰袋的保温箱中快速运输。

Then the animals were sacrificed and the surface preparation and the section of cochleae stained by MyosinⅥwere observed.Other cochlea were observed with SEM.

分别于术后第3.5个月分别行听性脑干反应检查后取双侧耳蜗标本做基底膜铺片和耳蜗冰冻切片MyosinⅥ染色观察有无新生毛细胞样细胞出现,部分耳蜗作扫描电镜观察。

No patient had neuropsychological deterioration, nor did any patient show side effects. Three patients were explanted after 2 years due to skin erosion in the trajectory of the system.

患者均无神经心理学病变,也未显示副作用。3例患者因刺激系统导线处皮肤溃烂于两年后移除深部脑刺激系统。

Methods: NG108-15 cells were cultured in vitro at 37℃in a humidified atmospherecontaining 5%CO_2. Aβ_(25~35) fragment was dissolved with deionized distilledwater and aged for 4 days at 37℃. The cells were exposured to 5μmol/LAβ_(25~35) for 24h to establish the cell model. Twelve rabbits were divided intotwo groups at random, and garaged with MWD or physiological saline controlrespectively for 7 days to collect two kinds of cerebrospinal fluid, theyare CSF from rabbits treated with MWD and CSF from rabbits treated withphysiological saline.

NG108-15细胞在37℃、体积分数为5%的CO_2培养箱中培养,Aβ_(25~35)溶解于双蒸水中,置于37℃培养箱中老化4d,使其寡聚,毒性增强,细胞被置于浓度为5μmol/L的Aβ_(25~35)中作用24h,建立起AD细胞模型。12只清洁级大耳白家兔购于华中科技大学同济医学院动物中心,将其随机分成两组,分别给与等量的加味温胆汤和生理盐水灌胃7d,末次给药后1h内用水合氯醛将给药组白兔麻醉,1.2ml/kg,无菌条件下用7号注射针头从枕骨大孔处垂直进针,抽取清亮含药脑脊液。

METHODS: Eleven specimens of medulloblastoma were acutely dissociated and triturated into single cells without trypsin digestion.

本研究旨在利用简化的无血清培养基和悬浮培养方法,从人髓母细胞瘤中分离培养脑肿瘤干细胞,观察其在体外的增殖、分化。

Three days after local cerebral infarction , the amounts of catecholamine-containing varicosities and the concentrations of CA in the varicosities in cortices of both sides were markedly reduced, and an aggregation of a large amount of CA granules which were of different fluorescent feature from tha...

局壮性脑梗塞三天后,双侧大脑皮质内CA膨体的数量与膨体内CA的浓度均显著减少,梗塞灶边缘区可见大量CA颗粒聚集,其荧光特征与正常皮质内的CA颗粒不同;双侧大脑皮质内的去甲肾上腺素含量显著减少,多巴胺含量无明显变化。

There was no difference about the maximum binding,affinity constant and concentration of the insulin receptor in cerebral cell membrane among the three groups.

三组大鼠比较,脑细胞膜的胰岛素受体最大专一性结合、受体亲和力常数及受体浓度均无显著性差异。

There were no death and severe stroke in the follow-up period (means 24 months).

随访46例,随访率88%,平均随访时间24个月,无死亡及严重脑卒中发生。

He was treated empirically with acyclovir, and multiple blood and CSF cultures were negative.

他在接受经验性的无环鸟苷治疗后,多次血培养和脑脊液培养是阴性。

According to the image manifest of DSA to analyze the size 、distributing、shape、aneurismal neck、having thrombus or not 、crevasse and blood stream conditi...

根据DSA的影像表现对脑动脉瘤的大小、分布、形态、瘤颈、有无血栓、破口及血流情况等进行分析。

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