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According to the formation of the apomitic embryos, apomixis could be divided into three major types: diplospory, apospory and adventive embryony.

按其胚体发生的途径,无融合生殖可分为二倍体孢子生殖、无配子生殖和不定胚生殖三种类型。

The results showed that there was no significant difference in three structure approach of liquid layers among which B was relatively higher;and it was considered that one-step dilution was an effective preservation method in that survival capacity with frozen and melted embryos freshly split,and survival capacity of frozen and melted IVF embryos split by one-step dilution by application minimum capacity refrigerant method with specific canaliculus were obviously higher than B.

结果,3种不同液体层构成间胚胎的存活能力无显著差异,B法较高;应用特定细管最小容量冷却法在冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚一步法稀释后的存活能力均显著高于B法。结果表明,特定细管最小容量冷却法是更有效的保存方法。

The results of biochemical analysis indicated that the content of GA 3 increased and the content of ABA decreased in cotyledons during the breaking of epicotyl dormancy, and the contents of endogenous growth regulators changed only slightly in the endosperm. We suggested that cotyledons be a key part controlling the epicotyl dormancy in peony seeds.

生化测定结果表明:在低温打破牡丹种子上胚轴休眠过程中,子叶中的萌发促进剂GA3 含量明显升高,抑制剂ABA 含量显著下降;而胚乳中的各种激素含量则变化不大,胚轴中的激素变化无明显规律,因此认为子叶是控制牡丹种子上胚轴休眠的关键部位。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

结果:与无上皮细胞相比,共培养明显促进了小鼠桑椹胚率(71.3%和61.2%,p.05),囊胚率(50.0%和39.7%,p.05),囊胚孵化率(34.8%和24.3%,p.05)和胚胎总细胞数(44.1和35.6,p.05)。结论:子宫内膜上皮细胞能够促进小鼠胚胎的体外发育,对早期胚胎的体外发育环境具有优化作用。

The morphology of M2 and M3 was observed in hothouse and mutants on cotyledon and root of seedling, or embryo of seed were identified. The result showed that there were 8 types of mutative treats on cotyledon, including light-yellow cotyledon, yellowish cotyledon, trump-shaped cotyledon, multilobed cotyledon, non-cotyledon, single-cotyledon, tricotyledon and quadrcotyledon.

M_2和M_3的水培和形态学观察的结果发现,子叶性状出现浅色、黄化、喇叭形、多耳突、无子叶、单子叶、三子叶、四子叶等8种变异类型;根系性状出现短小根、发达根、无侧根和弱向地性等4种变异类型;种胚性状中发现了多胚变异。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P<0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P<0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB,可明显提高双原核的形成率(38.9%vs 15.1%,P<0.05)和桑椹胚/囊胚发育率(40.7%vs 26.8%,P<0.05),但对其激活分裂率和总原核形成率无明显影响(P>0.05)。

Ovary superior, disk absent, locules and carpels 3-5 or more; placentation axile; ovules anatropous with a single integument, 10 or more per locule; styles as many as carpels, distinct or connate, generally persistent.

子房上位,无花盘,室和心皮3-5或更多;中轴胎座胚珠倒生,有单个珠被,每子房室有10个或更多的胚珠;花柱和心皮同数,离生或者合生,通常宿存。

To understand the degeneration of unfertilized aposporous embryo sac in an aposporous species Pennisetum squamulatum, the central cell in the unfertilized embryo sac was characterized ultrastructurally.

为理解植物无孢子生殖胚囊未受精条件下的退化,对无孢子生殖植物非洲狼尾草未受精成熟胚囊中央细胞退化做了细胞形态学研究。

This review is focused on the recent research progresses of related genes in the development of embryo, endosperm, and miosis, and several genes may involved in the regulation of apomitic development.

本文介绍了植物胚发育、胚乳发育、减数分裂等涉及无融合生殖过程的相关基因的研究进展,同时介绍了可能与植物无融合生殖途径调控相关的几个基因片段的研究情况。

When cultured on the medium with different auxin , root segments could elongate and branch, and the addition of 1mg/l IBA into the medium was the most suitable for long-term root culture. Calli could be induced from root explants in the presence of 2, 4-D and when transferred onto E medium, they transformed further into embryonic calli which could develop into normal plants through the stages of globular-embryo, heart-shaped embryo, torpedo-shaped embryo, cotyledonary embryo.

培养基中添加生长激素(IAA,IBA,NAA)能够诱导无菌苗根切段的生长和分枝形成;而1mg/l IBA的存在,最适合于根的长期离体培养。2,4-D的存在能够诱导根外植体脱分化形成愈伤组织,愈伤组织转移至E型分化培养基能够转变成胚性愈伤组织,并进一步经球形胚、心形胚、鱼雷形胚、子叶形胚发育成正常植株。

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