无胚的

Results:LIF improved the development rate of early embryo at the concentration of 0.1,1 and 10ng/ml(P.05).The enhanced effects of LIF were significant for morula and blastocyst stage,not for 1～8 cell stage of embryos.Conclusion:LIF may improve the development of early mouse embryos at the appropriate concentration.

结果：①0.1ng/ml、1ng/ml和10ng/ml浓度的LIF对小鼠早期胚胎的发育和胚泡的形成有促进作用，0.01ng/ml和100ng/ml浓度的LIF对胚胎的发育无明显影响；②LIF对小鼠早期胚胎发育的影响主要发生于桑椹胚和胚泡期，对1～8细胞期胚胎无明显影响。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测，最早在肢芽期胚胎中检测到2A3阳性信号；在膜内无节幼体期，2A3阳性血细胞平均直径约7μm，聚集成簇；在无节幼体Ⅰ期，血细胞形态更加完整清晰，平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现，这些细胞同样成簇存在，在其细胞质外围可见点状荧光。

When the embryonic original cell divided to form multicellur proembryos, the cell wall of the external cell was thickened to form a clear cell boundary to separate from ambient cells that was a phenomenon of isolation The protoderm began to be differentiated in the period of globular embryoid, and the procambium differentiated clearly in period of torpedo embryoid.

正常的球形胚和某些畸形球形胚继续发育，在心形胚或鱼雷胚阶段又出现出畸形胚，可以分为：①无茎端生长点；②原形成层发育不正常；③外形畸形，如有的是连胚，有的呈指状，有的呈叶片状等等。畸形胚有的败育，有的能继续发育，在子叶胚阶段形成畸形的植株。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Plantlet regeneration from cotyledon of Citrullus lanatus cv. Zhangkang No. 4 was studied. The results showed that aseptic seedlings should be cultured in dark for 3d, then exposed to a photoperiod of 16/8h/d for 3d. The callus induction rate was higher in cotyledons placed facing downwards to the medium than in those facing upwards. The highest induction rate occurred in MS medium containing 6-BA (2.0mg/L), kinetin (1.0mg/L)and GA3 (1.0mg/L). The induction initiated in dark and took 7 days, while callus growth and differentiation proceeding for 7 days under 16/8h light-dark cycles. A highest rate of embryogenic callus was obtained after 3 successive subcultures.

以无子西瓜郑抗4号无菌苗子叶为外植体，进行了体细胞胚发生及植株再生的研究，结果表明，无菌苗应先进行3d暗培养，然后采取光照16h/d和黑暗8h/d培养3d；将子叶的叶面朝下放置于培养基上的愈伤组织诱导率高于叶面朝上的培养方式；子叶诱导胚性愈伤组织的最适培养基配方为MS+6-BA2.0mg/L+KT1.0mg/L+GA31.0mg/L；诱导需要在黑暗条件下启动，进行7d暗培养，而生长分化于光照16h/d和黑暗8h/d条件下培养7d；继代3次得到的胚性愈伤组织最多；最适生根培养基配方为1/2MS+IBA0.3mg/L。

Stems angular, glabrous. Radical leaves: stipules brown, membranous, abaxially glabrous; petiole glabrous; leaf blade with 7–9 pairs of leaflets; leaflets petiolulate, green on both surfaces, fasciated or fasciated lanceolate, 5–7 × 1.5–1.7 cm, both surfaces glabrous, base subcordate to obliquely truncate or rounded, margin acutely incised serrate, apex rounded to acute; cauline leaves: stipules green, falcate, herbaceous, margin incised serrate; leaf blade resembling that of radical leaves but leaflets fewer and narrower higher up stem.

茎具角，无毛；胚根叶：托叶使成褐色，膜，背面无毛；叶柄无毛；叶片具7-9对小叶；具小叶柄的小叶，绿色在两面，扁化或者扁化， 5-7 * 1.5-1.7 厘米，两面无毛，基部近心形在斜截形或圆形，边缘尖锐锐裂状锯齿，先端圆形的到锐尖；茎生叶托叶绿色，镰刀形，草本，给锐裂状锯齿镶边；叶片象胚根叶的那但是高的更少和狭窄的小叶向上茎。

The expression of subunits α5,β1,β3 was detected in normal morulas and increased in blastocysts. The expression of subunit α1 was not detected in morulas or blastocysts, but detected only in outgrowing trophocytes. 3. Expression of integrin β3 in irradiated group embryos was weaker than that of normal group. There was no expression of integrin α1 both in morulas and blastocysts in irradiated group and no difference in the expression of subunits α5 and β1 between these two groups.

结果：1、超声波照射组胚胎贴附率、滋养细胞外延生长率均显著低于正常胚胎。2、正常胚胎桑椹胚期即有α5、β1、β3整合素表达，而无α1表达；囊胚期α5、β1、β3表达增强，仍无α1表达；α1只出现在外延生长的滋养细胞。3、超声波照射组胚胎β3整合素的表达明显弱于正常组，且未见α1表达；而α5、β1的表达两组间无差异。

Additionally, the water fraction from wood extract can inhibit the root growth on Pak-choi. At 1000 μg/mL, after 72 h, the length ratio of root to shoot was 0.69, and the dry weight of Pak-choi root was only the 20.6% of control. But the extracts of Sapindus mukorossi have no influence on the growth of Pak-choi with normal growth 7 days.

此外，木材抽出物之水分离部则具有抑制胚根生长的作用，经浓度1，000 μg/mL处理72 h之小白菜，胚根与胚茎长比值为0.69，且其胚根乾重仅为对照组的20.6%，但无患子抽出物对正常生长7天后之小白菜并无显著抑制生长作用。

METHODS: Constructing the CAM model by exposing CAM on the shell subpoint between 2 vitelline veins and generating an air chamber after 5 days of incubated with aseptic incubator at 37℃.

将鸡胚消毒后，置于37℃无菌恒温箱中孵育5 d，在距胚头1 cm两条卵黄静脉之间的卵壳投影部位磨切暴露尿囊膜，制成假气室，用无菌滤纸封闭窗口，制成鸡胚尿囊膜模型，备用。

It was confirmed that facultative apomixes is existed in coffee species based on emasculating treatment, cyto-genetic observe and SSR marker detection.

细胞遗传学观察发现咖啡四倍体类型材料(2n=4x=44)的多胚和多胚苗中含有天然的单倍体胚和单倍体小苗(2n=2X=22)；去雄处理后，可见子房膨大，其中5份材料获得了果实和种子，初步说明无融合生殖现象的存在；对小粒种品种间杂交F1代个体的SSR分析，发现多胚苗中存在与母本图谱相同且不携带父本特异带的后代以及携带双亲特异带的有性后代，表明子代有仅来自母本的无融合生殖个体分离出来。 3 建立了咖啡无融合生殖的分子鉴别方法。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

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起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。