无胚的

To isolate forebrain and hindbrain of human fetuses about 9 weeks and 12 weeks from spontaneous abortion. Neural stem cells were cultured in special culture medium and were compared with growth characterization.The differentiation from these neural stem cells was investigated in both a fetal bovine serum-containing and a non-supplemented,serum-free basal medium.

材料与方法：利用神经干细胞的条件培养基，对从9周和12周胚龄的自然流产胎儿分离的前脑、后脑进行神经干细胞培养，比较其生长特性，并分别观察在无生长因子的基础培养基以及含有胎牛血清的培养基中神经干细胞的分化情况。

A virus strain was isolated from the arthrosis of broiler chickens.The virus was shown to have spherical particles with isometric symmetry,no envelope,double deck capsids,with outer capsid of 75nm,inner one of 50nm in diameter by electron microscopy.The virus was not sensitive to aether,acid and heat.The virus could multiply on the chick embryo fibroblastcell,and could cause syncytial pathologic change with the appearance of cytoplasm inclusion bodies.The challenged chickens of one-day old by nasal,oral,intramuscular and pulvillus injection displayed obvious clinical symptom of severe arthritis and accompanied with pathological changes of malabsorption and necrosis.Meanwhile,four avian reovirus isolates CL,S1133,YH and YB were examined in the cross-neutralization test.

从临床患病鸡关节病料中分离到一株病毒，在电镜下观察到病毒粒子无囊膜，有双层衣壳，外衣壳直径约75nm，内核约50nm；分离病毒株对酸、热有较强的抵抗力，对乙醚不敏感；病毒感染鸡胚成纤维细胞上能产生以融合细胞为特征的CPE；爪垫接种1日龄雏鸡表现出明显的病毒性关节炎临床症状，并伴有吸收障碍型和坏死型的病理变化；血清中和交叉试验表明，该病毒分离株与国内的2株番鸭源呼肠孤病毒分离株和国外S1133呈不同程度的交叉反应。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示，在卵母细胞和8细胞期胚胎，基因calm3和trm112l的表达量无显著性差异，而在囊胚期的表达量与前两个时期的表达量存在显著性差异，推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控，提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

Female flowers: carpels 3, sessile; ovules ca. 20, in 2 rows on wall adjacent to ventral suture.

雌花 心皮3，无梗；约的胚珠20，在在腹面的缝附近的壁的2排。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。