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Under suspension culture without cytokines, the putative EG clonies could be induced to form simple embryoid bodies.

将消化分散的类EG细胞在无分化抑制因子的类EG培养基中重悬培养,3~4天后,可见部分细胞团形成简单的胚体。

The morphological and anatomical characteristics of"Zha′ai 76"Comparing with the plants of normal M.baccata and ordinary hybrid,"Zha′ai76"and other plants with Dw major gene showed that:the length of springgrowth or main shoot is shorter by 59.6-66.2%;the ratio of length:diame-ter of new shoot smaller by 33.2-58.4%;the length of internee shorter by14.0-42.7%:the node number of spring growth lesser by 40.7%;the cauli-cle height under the cotyledons of hybrid seedlings shorter by 65.0%;the in-dex of leaf stalk(stipe length:stipe thiek ness)on the spring growth smaller by34.5-50.2%;the length of leaf stalk shorter by 25.2-45.7%;the boundarybelt between spring and autumn growth has knob-like nodes;the margin of leafappears wave-like;the sepals with spinous proeessuses are massive and small;the eonstriction at calyx is not obvious;the trace of abscisic sepals is big;the ca-lyx end of fruit is plan or convex;the shape of shoot tip(of"Zha′ai 76")iswider and shorter;and in 1.0mm part from the top of shoot tip,there are manyspecialized cells which are larger in volarae,but with lesser inner material andwith smaller or no nucleus.

"扎矮76"的形态解剖特征与普通山定子和杂交后代中普通型植株对比,"扎矮76"和其它携带Dw主基因植株的:春梢短59.6-66.2%,梢长与梢粗比值小33.2-58.4%,节间短14.0-42.7%,春梢节数少40.7%,杂种苗下胚轴短65.0%,春梢叶柄形指数小34.5-50.2%,叶柄短25.2-45.7%,春秋梢交界处有瘤状突起,叶缘波状,萼片肥厚有刺状突起,萼部缢痕不明显,脱萼后萼痕较大、萼洼平或高于果面;新梢生长点宽而短,在距顶端1.0gmm处有较多体积较大、内容物较少、细胞核小或无的待化细胞。

Cytoembryological studies were employed to reveal theorigin of haploid and to determine the cytological factersrelating to the production of diploid polyembryony withinone embryo sac occurring in natural population of apolyembryonic line SB-1 of O.sativa.

SB-1自然群体中的单倍体来源于助细胞无配子生殖。细胞胚胎学研究表明,卵器中的一个或两个助细胞能在受精之前发育成胚,其发生率分别为观察子房数的2.24%和1.75%。

In the study, embryo bud of Impatiens balsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L+NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L+NAA 0.5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens baisamina L was finished in vitro.

研究以凤仙花无菌苗胚芽段作为外植体,经过不含任何激素的MS(0)培养基预培养、MS+6-BA3mg/L+NAA0.5mg/L培养基的诱导芽和根分化、MS+6-BA1mg/L+NAA0.1mg/L培养基的诱导开花,在60d左右实现了凤仙花离体培养再生植株并在试管内开花。

Ovary usually free, 2-5-locular, with 1 to many collateral or superposed ovules per locule; style single or rarely absent; stigma disciform or capitate.

子房通常离生,2-5室,每室有1到多数并生或者叠覆的胚珠;花柱单个的或很少无;柱头盘状的或头状。

Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differentiated.

体外分化采用无黏附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液,以脱饲养层法悬浮培养扩增的细胞,直到拟胚体形成及贴壁分化。

Ovary included by free perianth, sessile; style short; stigma ligulate, papillose on 1 side, persistent; ovule orthotropous.

离生花被包括的子房,无梗;花柱短柱头舌状,具小乳突的在一边上,宿存;胚珠直生的。

No longer is it the Substance that, as natura naturans, produces the world by a process of derivation and degradation without limit; it is an obscure germ, which in its ceaseless becoming, rises to the point of becoming man, and at that point becomes conscious of itself.

不再是它的实质内容,由于自然naturans ,生产世界上的过程,推导和退化无极限,它是一个晦涩的胚芽,在其不懈成为上升到如此地步,成为男子,在这一点上变得自觉管好自己。

Rough cloth comes in every day by an endless parade of wagons and it goes out as finished and dyed fabric the very highest quality. This wool will be made into the togas of senators.

每天都有无止尽的成排货车送进胚布,制成最高品质的染色精致织品送出,为参议院元老缝制精美的托加袍。

In de-embryonated seeds, it was demonstrated that the expression of at least one member of the LMW RNases could be restored by exogenous application of gibberellic acid.

外源赤霉素对于去胚乳的幼苗叶片RNase的表达可能无影响。

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