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Small intestinal biopsies showed subepithelial collagen deposition with varying degrees of villous atrophy and varying numbers of intraepithelial lymphocytes. Four patients had previous biopsies showing enteropathic changes without collagen deposition. Seven cases were associated with collagenous colitis and 1 also had features of lymphocytic colitis. Three patients also had collagen deposition in gastric biopsies. One case was associated with lymphocytic gastritis. Celiac disease (CD, gluten-sensitive enteropathy) was documented in 4 patients. Five patients made a clinical improvement with combinations of a gluten-free diet and immunosuppressive therapy. Two patients died of complications of malnutrition and 1 of another illness. Clonal T-cell populations were identified in 5 of 6 cases tested. Four of these patients improved clinically after treatment but 1 has died. Collagenous sprue evolved on a background of CD in 4 cases. There was no history of CD in others and these cases may be the result of a biologic insult other than gluten sensitivity.

小肠活检表现为上皮下胶原沉积,绒毛萎缩程度不一,上皮内淋巴细胞浸润数量不等。4例患者以往曾做过活检,表现为其他肠病改变,并没有胶原沉积。7例患者合并胶原性结肠炎,且1例还有淋巴细胞性结肠炎的特征。3例患者胃活检也发现有胶原沉积。1例患者合并淋巴细胞性胃炎。4例患者有乳糜泻(CD,谷蛋白敏感性肠病)病史。5例患者经无麸质饮食和免疫抑制治疗后,临床症状有所改善。2例患者因营养不良合并另一种疾病而死亡。6例患者做了T细胞受体基因重排,其中5例发现有克隆性T细胞群,这5例中有4例治疗后临床症状加重,且1例死亡。4例患者在CD的基础上病发胶原性口炎性腹泻;其余患者无CD病史,他们的发病也许是生物源性损害,而非谷蛋白敏感。

The statistical comparison was used chi-square test between the methanal or hydrochloric acid abdominal cavity injection method and Galili's method.

结果 大鼠在腹腔注射1%甲醛与0.5%盐酸后均100%发生肠粘连,注射0.5%甲醛的大鼠肠粘连的发生率为87.5%,且0.5%盐酸,1%甲醛,0.5%甲醛腹腔注射法与Galili法相比,差异无显著性。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测,最早在肢芽期胚胎中检测到2A3阳性信号;在膜内无节幼体期,2A3阳性血细胞平均直径约7μm,聚集成簇;在无节幼体Ⅰ期,血细胞形态更加完整清晰,平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现,这些细胞同样成簇存在,在其细胞质外围可见点状荧光。

Methods This study consisted of 36 male Wistar rats. A rat model of intestinal adhesions was done as follows: serous membrane of the caecum was scuffed, its raw surface was dropped with absolute alcohol, the mesentric artery of the caecum was then clamped. The rats were divided into three groups. PSS, sodium hyaluronate or normal saline was applied to the scraped cecum before closing the abdomen.

选用雌性Wistar大鼠36只,刮伤其盲肠浆膜,创面滴无水乙醇,钳夹盲肠系膜动脉制造大鼠肠粘连的动物模型,随机将动物分成3组,分别在关腹前于创面涂布藻酸双酯钠、透明质酸钠、生理盐水,术后14 d将大鼠处死,肉眼观察肠粘连程度并对粘连组织的基质金属蛋白酶2和金属蛋白酶组织抑制物2水平进行检测。

Results: The expression of NGF mRNA in aganglionic and hypoganglionic colon(2/9) was less than that in the proximal normal colon(8/9) and normal control.

结果: 无神经节细胞的巨结肠狭窄段和节细胞过少的肠组织与扩张段近端正常肠组织相比,其NGF mRNA表达水平降低,阳性率分别是2/9和8/9,肠神经元发育不良的病变肠组织NGF mRNA表达水平亦有下调趋势。

Results: The expression of NGF mRNA in aganglionic and hypoganglionic colon(2/9) was less than that in the proximal normal colon(8/9) and normal control. The IND specimen also showed decreased expression.

结果: 无神经节细胞的巨结肠狭窄段和节细胞过少的肠组织与扩张段近端正常肠组织相比,其NGF mRNA表达水平降低,阳性率分别是2/9和8/9,肠神经元发育不良的病变肠组织NGF mRNA表达水平亦有下调趋势。

Many gap junctions were observed between Interstitial cells of Cajal andsmooth muscle cells, between smooth muscle cells and smooth muscle cells in ganglionic part of HD bowel,which was absent or less in the aganglionic part.

Cx43在HD无神经节细胞肠段表达缺失提示细胞间通讯的破坏可能与HD肠功能异常有关。

No matter whether the patients of pancreatic diseases have enteroparalysis or not,the enteral nutrition should be administrated at onset or within 24-48 hours postoperatively.

也有学者认为:避开胃和十二指肠的肠内营养无显著加重胰腺损害的危险,胰腺疾病患者无论是否存在肠麻痹,发病或手术24-48小时即可给予肠内营养支持。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌,在不同时相处死后,观察肠内白念菌总数及肠粘膜表面白念菌粘附数量;取肠系膜淋巴结做白念菌选择培养,观察移位体内发生率;采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况;流式细胞计数潘伊尔氏结细胞表面IgA阳性率;免疫组化染色后计数固有层中IgA浆细胞数量变化;ELISA法测定肠粘液中特异抗白念菌IgA含量。

RESULTS: Muscular atrophy was observed 2 weeks after denervation. The motor unit waveshape of gastrocnemius became single; time limit became long; voltage became low, and the fibrillation potential norientation wave was increased. Differences were observed between two groups 4 weeks and 8 weeks, but no differences were observed after 12 weeks.

结果:①切断坐骨神经后2周大鼠腓肠肌均发生不同程度的萎缩「左侧腓肠肌运动单位电位波形逐渐变单一,时限逐渐延长,电压逐渐变小,纤颤电位的正向波逐渐增多。4、8周时实验组与对照组有差异,12周时两组无差异。

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