无结果的

The researching result of this field is conflicting in these years. Some researchers consider it as apoptosis, while some necropsy results don\'t support the hypothesis. Our study is to investigate the protein expression and biochemistry changes under oxidative stress to determine if apoptosis happens.

多巴胺能神经元的死亡是凋亡还是坏死目前的研究结果不一，有许多的研究说明在氧化应激的条件下，细胞的死亡是凋亡，而部分尸检结果不支持凋亡的学说，本实验拟从蛋白表达及生化学方面观察氧化应激后细胞有无凋亡的发生。

The main results can beexpressed as follows:(1)Based on πtheory, some affected factors about wave forces are given, and therelation between affected factors and wave forces is analysised by experimentalresults.(2) The distribution of wave press at representative time and reflection nature aregiven and their variety charactes are discussed by experimental results.(3)By comparing the wave forces of quarter-circular breakwater and semi-circularbreakwater under the same wave conditions,some approximately wave forcesexpressions of quarter-circular breakwater are given.(4)Based on VOF method to trace water free surface,solve numerical value ofRANS equation,and get the distributions of flow field and wave press ofquarter-circular breakwater at the same time during one period,the mechanism ofwave force is also discussed elementarily.(5) Based on the result that RANS equation of numerical simulation agree wellwith experimental results,a new approach to study hydraulic natures of quarter-circular breakwater is provided.

其主要结论如下：一、通过π定理，确定影响波浪力的无因次参变量，根据试验结果对其影响因素进行分析；二、基于模型试验，给出典型时刻圆弧面防波堤的同步波压分布和反射特性，讨论它们的变化特点；三、通过相同条件下圆弧面防波堤与半圆型防波堤两种堤型试验波浪力的比较，给出了圆弧面防波堤波浪力的近似方法；四、利用VOF法追踪自由水面，数值求解RANS方程，得到圆弧堤堤体周围的流场和压力场，初步分析了速度涡流与压力场的相互影响；五、对比数值分析与试验结果，探讨数值方法研究圆弧面防波堤水力特性的可行性，提供新的研究途径

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性，进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠，3次免疫小鼠后，血清(1：1000)中能检测到较强的MUC1抗体阳性；以最大OD450值之纯化血清做为一抗行蛋白印迹分析，结果显示：纯化血清可识别MUC1蛋白；小鼠脾脏淋巴细胞增殖率检测结果表明：噬菌体展示肽表位可在无佐剂存在情况下，大大促进小鼠的脾淋巴细胞的增殖，证明噬菌体是一较好的生物载体。

The result shows that the changing law of saliferous reservoir parameters is a nonlinear relationship with several variables, and it is a very complicated process.

研究结果表明，高含盐油藏储层参数变化呈多元非线性函数关系，变化规律复杂；渗透率变化存在临界值，大于临界值时，渗透率随着驱替速度、驱替量和含盐量的增加而增大，小于临界值时，渗透率随着驱替速度、驱替量和含盐量的增加而减小；注入水性质会对参数变化过程有影响，而对最终变化结果无影响；盐的溶解是储层参数变化的根本原因，岩石颗粒的脱落和运移是储层参数变化的直接原因。

Methods The primer design is referring to thearticleof Bulter. Over 120 samples provided by the laboratoryare detected to verify the concordant results between miniSTR andcommon STR. We construct the miniSTR multiplex system usingdomestic Taq DNA polymerase, and detect the results by ABI—310Genetic Analyzer. We have alsostudied the sensitivity, accuracy, species-specificity and different PCR outcome under each annealtemperature.

方法参照Bulter等对THO1、TPOX、CSF1PO三个基因座miniSTR的引物设计，选用本教研室提供的123例无血缘关系个体的血样，进行THO1、TPOX、CSF1PO三个基因座PCR-miniSTR银染检测，检测结果与商品化试剂盒的检测结果进行一致性检验；采用国产DNA聚合酶构建miniSTR荧光标记复合扩增体系，用美国ABI-310基因分析仪进行检测；对该荧光标记复合扩增体系的灵敏度、准确性、种属特异性、不同退火温度下复合扩增的效果、陈旧血痕DNA的检测等进行研究。

The microstructure of MDF and MDF tested were studied. The results showed that the fibers in MDF are arranged randomly. Some of them arranged separately, most of them arranged in bundles. There are many blind holes between the fiber cells in MDF. The damage of MDF's compressive creep is the result of new cranny occuring, spreading and the combination of old cranny. In the repressing process, besides the pressure, heat is also the main factor on the damage of MDF, which can accelerate the adhension layer to deteriorate and lead to the gule strength decreasing.

结果表明：MDF的纤维排列无规则，纤维排列形态除少量纤维呈单根状外，大量的纤维以纤维束的形态排列；纤维之间存在许多大小不等的孔洞，呈不贯通状；MDF蠕变损伤是不断萌生新的裂隙并扩展和合并原有裂隙的结果；MDF二次加工过程中，除了压力是破坏MDF结构的主要因素外，加热温度使MDF胶层老化，胶合强度下降，也是破坏MDF结构的主要因素；MDF的破坏形态除了有纤维拉断外，还有纤维拔出和胶粘剂脱落。

In result 2.5% of the rats got slight hepatic enzymes increase, and 6.25% of the rats got slight fat degeneration for hepatic tissue specimens. Most of the rats with abmormal results occurred in the toxic and lethiferous group, and there was the increasing tendency with the increase of dose. There were no changes for renal function and tissue biopsy.

实验结果显示共有2.5％的小鼠出现了轻度的肝酶升高，组织病理有6.25％的小鼠肝组织出现了轻度的脂肪变性，结果异常的小鼠绝大多数发生于中毒剂量及致死剂量组，且有随着剂量增高而增加的趋势；肾功能及组织病理无明显改变。

Through designing of composition and structure of the bioactive graded coating,innerstress and its distribution in the coating were analyzed and calculated, the resultsshowed that when composition distribution coefficient n was 1.5, a reasonable stressdistribution could be got, that was at the beginning of deposition the suspension containingrichly BG granules was used so that a rich BG granules layer, a good transitional layerbetween BG layer at the bottom and the coating could be obtained at the titanium alloy side,the bottom of the coating; the stress value near the interface and surface and its character,pressure stress or tensile stress, were decided by the character of its composition itself.Changing composition distribution coefficient n could only change the variation tendency ofstress in the coating, but did not change the stress distribution rule in the coating. Thethinner the coating is, the sharper stress variation in the coating is, which does not mean thatthicker coating is better because the thicker the coating is, the little the permitteddeformation of coating is, so the coating thickness should be thinner, for example, about50μm for bending applications, but for applications only bearing pure shear stress, such asroot of tooth implant, the coating can be thicker little, for instance, about 80～100μm. The study on electrification characteristic and electrophoresis deposition of HAand BG granules in aqueous and non-aqueous solution system found that EPD almost didn'toccur in aqueous solution system. However, because HA granules take position charges inabsolute alcohol, a homogeneous EPD be carried out on the cathode titanium alloy slice, but taking negative charge in absolute alcohol the BG granules not be deposited on the cathode. A guided HA crystallizing, 100～300nm, on surface of the BG granules be realized by metathetical reaction, which cover BG granules with HA microcrystals and make the covered BG granules taking position charges in absolute alcohol, sequentially realize the EPCD of the BG and HA granules on the cathode, so it is feasible to make a titanium alloy/BG/HA bioactive graded coating by making use of EPCD technology. The corrosion experiment of rich boron bioglass coating and plasma spray coating showed that split phase, rich boron and rich silicon phase, occurred during its preparation. In basic medium the corrosion behavior of 〓 BG coating showed uniformity corrosion, the corrosion mostly occurred at rich boron phase area, therefore batch formula design of BGshould avoid the occurring of split phase. The corrosion appearance of plasma spray coatingappeared a non-uniform corrosion, mostly occurred at the edge of the laminated HA moltendrops, and emerged an accelerated corrosion tendency, which will easyly lead to corrosioncrackles extending to the interface and the happening of osmotic interfacial corrosion, thatmay be one of the major reasons leading to the coating cracking-off in the later period. Thetesting results of thermal expansion coefficient of 〓 and 〓BG showed the thermalexpansion coefficient of 〓 BG matched with that of titanium alloy better, and 〓 BG couldsinter with titanium alloy into densification enamel layer at low temperature (720℃).

将Ti6Al4V合金在1000℃下进行真空热处理会降低其力学性能，且合金内的V元素会向表面富集，因此，钛合金真空热处理和表面涂层的烧结温度不能过高，即应低于其相转变点；通过对生物活性梯度涂层的组成和结构的设计，分析和计算了梯度涂层内的应力大小和分布，结果表明：对于本研究，当成分分布系数n=1.5时，可以获得较合理的涂层力学性能，即在沉积开始时，采用富含BG颗粒的悬浮液，以便在钛合金侧获得同底层BG有良好过渡的富BG涂层；梯度涂层界面和表面的应力大小、性质由材料组成本身的性质决定，改变成分分布系数，只能改变涂层内应力变化的趋势；涂层的厚薄不影响涂层内的应力分布规律，但涂层越薄，涂层内的应力变化越快，但这并不意味着涂层越厚越好，因为涂层越厚，涂层允许的变形越小，对于应用于弯曲受力部位的涂层而言，涂层应薄一点为好(50μm)；而对于仅纯受剪切应力的部位，如牙根种植体，涂层可适当加厚(80～100μm)；通过对HA和BG颗粒在水溶液体系和非水溶液体系中的带电特性和电泳沉积的研究发现，它们在水溶液体系中很难发生电沉积；在无水乙醇溶液中，HA颗粒带正电，可在阴极钛合金片上发生均匀的电泳沉积，而BG颗粒则带负电荷；利用复分解反应法，可以制得100～300nm的HA，通过诱导HA在BG颗粒表面结晶，可对BG颗粒进行表面包覆，获得了被HA包覆的BG颗粒，改变了BG颗粒表面的带电特性，使BG和HA颗粒在无水乙醇中均带上正电荷，从而实现了HA和BG颗粒在阴极上的共沉积。

This method consists of two steps: first, the image is divided into numbers of nonoverlapping blocks and the GMRF parameters are computed for each of these blocks, then a clustering method is used to merge these blocks, a coarse segmentation and texture types of the image are obtained. Second, a deterministic relaxation neural network is constructed, the parameters of the model estimated from the first step are used as input data, through neural network computation, a fine segmentation is obtained.

提出一种基于高斯-马尔可夫随机场和神经网络的无监督纹理分割方法，方法分为两步：第一步先将图像分为不重叠的小块，在小块中计算GMRF参数，并将此参数和该块的均值、方差作为该块的特征向量，然后进行聚类，得到原图像的一个初始分割和图像中所包含的类别数；第二步构造一个决定性松弛的神经网络，将第一步得到的结果作为初始输入，经过神经网络计算，得到一个精确的分割结果。

Results:Comparing to the control group, the proliferation of overexpressed p16 QBC939 cells was inhibited and p16 overexpress induced G1 phase blockade.

结果：与对照组相比，p16高表达的人胆管癌细胞QBC939的增殖受到明显抑制；流式细胞光度术表明p16的高表达导致了较为明显的G1期阻滞作用，但对细胞周期的其他时相并无影响；Western免疫印迹分析结果表明癌基因c-myc的蛋白水平表达下降。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。