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Further analysis shows that the AT determined in the method of unprogressive increasing load is a mere scientific reflection of the real level of the body and gives a mere scientific basis for estimating an athlet's aerobic endurance and for guiding sports training.

结果无累进递增负荷的AT值明显推迟出现;进一步研究发现采用无累进递增负荷测定的AT值更能科学地反映机体的实际水平,能对评价运动员有氧耐力水平和指导运动训练提供更科学的依据。

As a result,the apearance of AT in running with an unprogressive increasing load is abviously later than that in running with a progressive increasing load.Further analysis shows that the AT determined in the method of unprogressive increasing load is a more scientfic reflection of the real level of the body and gives a more scientific basis for estimating an athlet's aerobic endurance and for guiding sports training.

结果无累进递增负荷的AT值明显推迟出现;进一步研究发现采用无累进递增负荷测定的AT值更能科学地反映机体的实际水平,能对评价运动员有氧耐力水平和指导运动训练提供更科学的依据。

The results show that there were no significant differences among the four forage rations in rumen pH, the concentrations of total volatile fatty acids, acetate, propionate, the ratios of acetate to propionate, butyrate, isobutyrate and valerate(P﹥0.05). Only in 11:30, the concentrations of isovalerate caused by ration A and B were significantly higher than the other two rations(P﹤0.05).The milk yield caused by the ration C was significantly lower than the others (P﹤0.05). However, there were also no significantly differences among these four rations in the DM, fat, sugar, protein and scc of the milk. The milk fat and sugar of ration B and C were higher than ration A and D in number as well.

试验结果显示:各粗料组合日粮对各个时间点的瘤胃发酵指标:pH、TVFA、乙酸、丙酸、乙酸/丙酸、丁酸、异丁酸、戊酸浓度均无显著差异(P﹥0.05),只在11:30时,日粮A、B的异戊酸浓度显著低于日粮C、D (P﹤0.05);日粮C的产奶量显著低于其它三个日粮,而在乳干物质、乳脂、乳糖、乳蛋白、体细胞数上,各日粮均无显著差异,从数值上看,日粮B、C的乳脂和乳糖高于A、D。

The results of toxicity experiments showed that R32 would not cause acute, chronic, cumulative and genetical toxicity effect on algae, Allium cepa, Zebra, fish and mice before it absorbed Cr. The recovery and sterilization measurement in routine water treatment could reduce most of the R32 in effluent. This may ensure that the effluent would not cause any ill effect on all kinds of biology in the environment after it was discharged into the river.

毒性实验结果表明:在吸附Cr~(6+)以前R_(32)对微生物无致突变性,对藻类、洋葱、斑马鱼和小鼠无急性毒性、慢性毒性和蓄积毒性;吸附Cr~(6+)后,常规水处理中的各种回收和杀菌措施可以降低出水中R_(32)的量,使出水排入江河后不会对环境中的各种生物产生不良的影响。

The cancers studied here were throat cancer, aute non- lymphoblas He leukem ia and acut lymphoblastic leukemia. The result s indicated that,(1)the levels of Leu, Lys, Ser Asn,Ile,Gly,and Thr were obviously decreased whea lse the levels of orn, Trp evidently increased in cultural liquid of throat can cer cell line indicating that the growth of HepⅡ cell consumed 7 amino acids and releaced 2 amino acids into cultural liquid.(2)the levels of Glu, Gls, Leu, Phe, Tyr and Trp were evidently increased while the levels of Thr , His, Ala decreased in plasma from patient with ANLL. These r esults were consistent with the previous report.(3)the levels of Gly, Trp and Phe in plasma from patients with ANLL evidently de creased and the levels of Ala, His increased after therapy indicating that cance r cells were growing in an anaerobic catabolism state.

这些癌肿包括喉癌HepⅡ细胞,急性非淋巴细胞白血病和急性淋巴细胞白血病,结果表明:(1)喉癌细胞株培养过程中亮氨酸、赖氨酸、丝氨酸、天冬酰胺、异亮氨酸、甘氨酸以及苏氨酸等水平明显降低,而色氨酸水平明显增加,说明喉癌细胞的生长繁殖必须依赖以上7种氨基酸同时释放了色氨酸;(2)急性非淋巴细胞白血病患者血浆中的谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸等水平明显升高,而苏氨酸、组氨酸、丙氨酸等水平明显降低,这些结果与国际报道相一致;(3)经治疗后,ANLL患者血浆中甘氨酸、色氨酸和苯丙氨酸等水平明显降低,而丙氨酸、组氨酸等水平明显升高,表明肿瘤细胞处在无氧代谢。

Total of 240 choosable cases including 120 males and 120 females with HCT parameters 0.42L/L and 270 choosable cases including 150 males and 120 females with HCT parameter 0.45L/L,examined by pressure transfer type viscometer from healthy testers.

结果不论是锥板式还是压力传感式血流变仪的测定结果,各相同HCT组的高、中、低切全血粘度值按不同性别比较均无显著性差异(P>0.05)。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实:两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用,并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著,最佳效应浓度为400nM;2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响:①CREG蛋白对VSMC迁移的影响:刮伤实验发现,加入最佳效应浓度的wtCREG和mCREG蛋白24h后,OB2组迁移能力下降,HITASY组无明显变化;细胞外基质金属蛋白酶-2,9(Matrix metallo-proteinase 2,9,MMP2 ,9)明胶酶电泳检测和Western blot检测结果证实,两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2,9减少,而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases,TIMPs)增加;②CREG蛋白对VSMC分化的影响:加入400nM的wtCREG和mCREG蛋白12h后,OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加,LM-1、FN表达减少;③流式细胞仪分析细胞周期和BrDU染色分析证实,加入400nM的wtCREG和mCREG蛋白后,OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572,HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034;3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用:①免疫共沉淀和免疫荧光双染色分析结果显示,CREG蛋白与M6P/IGF2R存在直接结合;②应用抗体阻断实验:将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中,CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱,而且与加入anti- M6P/IGF2R浓度正相关。

Objective:To evaluate the application of titanium plate and titanium mesh in multi-segmental cervical spondylotic myelopathy anterior fixation.Methods:Twenty-five patients of multi-segmental cervical spondylotic myelopathy were treated with anterior cervical corectomy and fixation with titanium plate and titanium mesh from 1998 to 2002.Results:All the twenty-five patients of bone planted were in fusion.Titanium mesh shedding,screw loose,plate breaking were not found after follow-up for an average of twelve months.The function of nerve had been improved.

中文摘要:目的:探讨前路钛钢板加钛网植骨内固定治疗多节段脊髓型颈椎病的效果及意义,方法:总结1998年至2002年期间收治的25例有2-3个节段突出的脊随型颈椎病行前路椎体次全切术前路钛钢板加钛网植骨内固定术,结果:经平均12个月的随访,25例患者植骨全部融合,无钛网移位脱落,无螺钉松动,钢板断裂,神经功能得到了不同程度恢复。

objective to study the effect of qingshanjianxin tablet on treating arrhythmia.methods sixty wistar mouse were ransdomed into six groups, aconitine evocate arrhythmia, and continuously observe the changes of electrocardiogram and record the latent period, the lasting time of the arrhythmia and the death rate.results qingshanjianxin tablet can prolong the latent period of the arrhythmia, shorten the lasting time of arrhythmia and lower the death rate.

目的 研究青山健心片抗心律失常的作用机制。方法采用乌头碱诱发wistar大鼠心律失常,持续观察心电图变化直至出现心律失常,记录心律失常的潜伏期、持续时间及死亡率。结果青山健心片高、中剂量组均能延长心律失常的潜伏期,缩短心律失常的持续时间,降低死亡率,与心律平片组无明显差异(p>0.05),疗效优于低剂量组、空白对照组、心律宁片组(p<0.05),高剂量组与中剂量组之间无显著差异(p>0.05)。

The mainly results of this work are as follows: 1. To determine the genetic characterize of Gl_2~e gene, we investigated the different positions and stages of 1599 F_2 segregating plants. The results showed that 441 plants had dark-colored glands; 427 glandless plants and 761 plants with low-glands respectively.

本研究结果如下:一、分别对不同生育时期F_2群体单株各部位性状调查发现,表现为TM-1有腺体性状的411株,表现为海_1无腺体性状的427株,与F_1一样少腺体的761株,经检验符合1:2:1的分离比例X~2=4.028<X~2_(0.05,2=5.99,进一步证明海_1无腺体性状是由一对不完全显性基因Gl_2~e控制。

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